Abstract:
They young leaves of cucumber, eggplant, lettuce, Chinese cabbage, Chinese radish and onion are made into squashes and then stained with a new single stain PIHCH (propionic-iron-haematohylin-chloral hydrate) and a compound stain of PIHCH and PICCH (propionic-iron-camine-chloral hydrate). The chromosomes which are hardly stained with common stains can be clearly observed under the microscops. Therefore the single stain PIHCH and the compound stain of PIHCH and PICCH can be widespreadly applyed in cytology study.
Key words:
Stain PICCH,
Young leaves of vegetable,
Chromosomes,
Compound staining
YangPeiru, WangRuihuan, ZhangJunming, ZhangShu'e. A New Technology of Compound Stain Squash and a New Stain for Identification of Chromosomes[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(3): 61-64. doi: 10.3321/j.issn:1000-7091.1991.03.011.