Abstract: Microspore embryogenesis from two autumn cauliflower genotypes was induced by anther float cultures on a modified B5 liquid medium. The liquid medium seemed to be more suitable for cauliflower anther culture in comparison with the solid medium. There were similar embryo yields between the single liquid medium and the double layer medium. However, the brown embryo rate of the latter was very high. Comparison among the different AgNO3 concentrations in the single liquid medium showed that 62.5mg/L AgNO3 was the optimum concentration for microspore embryogenesis. It was different from that on solid anther culture (125mg/L). The results from the experiments suggested a possible use of the anther float culture method for haploid production in cauliflower breeding.

Key words: Cauliflower (Brassica oleracea var,botrytis), Anther float culture, Microspore embryogenesis