燕麦<i>AsCHS</i>基因克隆和表达分析

doi:10.7668/hbnxb.20195516

摘要/Abstract

摘要：

查尔酮合成酶(CHS)是类黄酮合成途径的关键起始酶,参与合成黄酮、黄酮醇、异黄酮、花青素等代谢物,而这些黄酮类代谢物在植物抗逆方面扮演着重要角色。为了研究CHS在燕麦幼苗响应干旱胁迫中的作用,从前期的全长转录组数据中筛选到1个燕麦CHS基因,命名为AsCHS,并对其进行克隆、生物信息学、亚细胞定位和表达模式分析。结果表明,AsCHS基因编码398个氨基酸,蛋白序列包含CHS家族特异标签序列,是疏水的不稳定蛋白,属非跨膜蛋白,定位于细胞核和细胞质。根据二级结构预测,AsCHS主要包含α-螺旋和无规则卷曲。启动子区顺式作用元件预测表明,该基因含有响应干旱胁迫和多个激素信号途径相关的顺式作用元件。系统进化树显示,AsCHS与黑麦草、早熟禾和南极发草的亲缘关系较近。亚细胞定位表明,AsCHS蛋白定位在细胞核和细胞质。与对照组相比,AsCHS在干旱胁迫下燕麦幼苗不同萌发时间的表达模式由波动表达改变为递增表达,由根中表达量最高改变为在叶中表达量最高,且在叶片中的表达存在显著差异。本研究为揭示AsCHS在燕麦响应干旱胁迫的功能奠定了基础。

关键词: 燕麦, 查尔酮合成酶, 干旱胁迫, 表达

Abstract:

Chalcone synthase(CHS)is the initial and crucial enzyme in the flavonoid biosynthesis pathway,responsible for the synthesizing of metabolites such as flavones,flavonols,isoflavones,and anthocyanins,which play a vital role in enhancing plant stress resistance.In order to explore the role of CHS genes in the drought stress response of oat seedlings,it identified a CHS gene from the full-length transcriptome data of oats,named AsCHS.Gene cloning,bioinformatics analysis,subcellular localization,and expression pattern analysis were conducted.The results showed that the AsCHS gene encoded a protein composed of 398 amino acids and had a CHS family-specific tag sequence.This protein was hydrophobic and unstable.It was a non-transmembrane protein and was located in the nucleus and cytoplasm.Secondary structure prediction showed that AsCHS was mainly composed of α-helices and random coils.The analysis of the cis-acting elements within the promoter region revealed that the gene contained cis-elements associated with drought stress response and multiple hormone signaling pathways.Phylogenetic tree analysis showed that AsCHS was closely related to its counterparts in Lolium perenne,Poa annua,and Deschampsia antarctica.Subcellular localization indicated that the AsCHS protein was localized in the nucleus and cytoplasm.Compared with the control group,the expression pattern of AsCHS in oat seedlings under drought stress changed from fluctuating expression to incremental expression with different germination time,shifting from the highest expression level in roots to the highest in leaves,with significant differences observed in leaves expression.It laid a foundation for elucidating the function of AsCHS in the drought stress response of oats.

Key words: Oat, Chalcone synthase, Drought stress, Expression

中图分类号:

S512.6燕麦

安江红, 王力伟, 斯钦巴特尔, 孙华, 赵梦然, 孙天昊, 何江峰, 赵杰. 燕麦AsCHS基因克隆和表达分析[J]. 华北农学报, 2025, 40(4): 41-48. doi: 10.7668/hbnxb.20195516.

AN Jianghong, WANG Liwei, SIQIN Bateer, SUN Hua, ZHAO Mengran, SUN Tianhao, HE Jiangfeng, ZHAO Jie. Cloning and Expression Analysis of AsCHS in Oat[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 41-48. doi: 10.7668/hbnxb.20195516.

http://www.hbnxb.net/CN/Y2025/V40/I4/41

图/表 9

参考文献 31

[1]	蔺军. 黑麦和燕麦种子萌发期耐盐性、抗旱性比较[J]. 中国草食动物科学, 2016, 36(4):31-34.doi:10.3969/j.issn.2095-3887.2016.04.009.
	Lin J. Salt tolerance and drought resistance of Secale cereal and Avena sativa during the seed germination stage[J]. China Herbivore Science, 2016, 36(4):31-34.
[2]	赵小娜, 高志昊, 王斌, 张译尹, 胡海英, 兰剑. 15份饲用型燕麦种质材料苗期抗旱性比较与评价[J]. 草地学报, 2023, 31(12):3734-3743.doi:10.11733/j.issn.1007-0435.2023.12.018.
	Zhao X N, Gao Z H, Wang B, Zhang Y Y, Hu H Y, Lan J. Comparison and evaluation of drought resistance of 15 forage-type oat germplasm materials at seedling stage[J]. Acta Agrestia Sinica, 2023, 31(12):3734-3743.
[3]	李彦霞, 周海涛, 刘文婷, 李天亮, 杨晓虹, 张新军. 干旱胁迫下不同燕麦品种光合荧光特性及其抗旱性评价[J]. 种子, 2021, 40(2):26-34.doi:10.16590/j.cnki.1001-4705.2021.02.026.
	Li Y X, Zhou H T, Liu W T, Li T L, Yang X H, Zhang X J. Photosynthetic fluorescence characteristics and drought resistance evaluation of different oat varieties under drought stress[J]. Seed, 2021, 40(2):26-34.
[4]	吴雨涵, 刘文辉, 刘凯强, 张永超. 干旱胁迫对燕麦幼苗叶片光合特性及活性氧清除系统的影响[J]. 草业学报, 2022, 31(10):75-86.doi:10.11686/cyxb2021410.
	Wu Y H, Liu W H, Liu K Q, Zhang Y C. Effects of drought stress on leaf senescence and the active oxygen scavenging system of oat seedlings[J]. Acta Prataculturae Sinica, 2022, 31(10):75-86.
[5]	Tian H Q, Zhou Q P, Liu W H, Zhang J, Chen Y J, Jia Z F, Shao Y Q, Wang H. Responses of photosynthetic characteristics of oat flag leaf and spike to drought stress[J]. Frontiers in Plant Science, 2022,13:917528.doi:10.3389/fpls.2022.917528.
[6]	赵宝平, 任鹏, 徐忠山, 武俊英, 刘瑞芳, 刘景辉. 水分胁迫对不同抗旱性燕麦品种光合及产量形成的影响[J]. 麦类作物学报, 2020, 40(11):1399-1407.doi:10.7606/j.issn.1009-1041.2020.11.15.
	Zhao B P, Ren P, Xu Z S, Wu J Y, Liu R F, Liu J H. Effects of water stress on photosynthetic characteristics and yield formation in oats(Avena sativa L.) with different drought resistance[J]. Journal of Triticeae Crops, 2020, 40(11):1399-1407.
[7]	刘凯强. 水分胁迫对燕麦生长发育及产量构成的影响[D]. 西宁: 青海大学, 2020.doi:10.27740/d.cnki.gqhdx.2020.000388.
	Liu K Q. Effects of water stress on growth and yield components of oats[D]. Xining: Qinghai University, 2020.
[8]	Zhang X J, Liu W T, Lv Y C, Li T L, Tang J Z, Yang X H, Bai J, Jin X, Zhou H T. Effects of drought stress during critical periods on the photosynthetic characteristics and production performance of Naked oat(Avena nuda L.)[J]. Scientific Reports, 2022, 12(1):11199.doi:10.1038/s41598-022-15322-3.
[9]	徐忠山. 利用多组学联合分析燕麦响应干旱胁迫及盐胁迫的分子机制[D]. 呼和浩特: 内蒙古农业大学, 2022.doi:10.27229/d.cnki.gnmnu.2021.001232.
	Xu Z S. Using multi-omics to analyze the molecular mechanism of oat in response to drought stress and salt stress[D]. Hohhot: Inner Mongolia Agricultural University, 2022.
[10]	Larsson K E, Nyström B, Liljenberg C. A phosphatidylserine decarboxylase activity in root cells of oat(Avena sativa)is involved in altering membrane phospholipid composition during drought stress acclimation[J]. Plant Physiology and Biochemistry, 2006, 44(4):211-219.doi:10.1016/j.plaphy.2006.04.002.
[11]	Zhang X J, Liu W T, Lyu Y C, Bai J, Li T L, Yang X H, Liu L T, Zhou H T. Comparative transcriptomics reveals new insights into melatonin-enhanced drought tolerance in naked oat seedlings[J]. PeerJ, 2022,10:e13669.doi:10.7717/peerj.13669.
[12]	Sun M, Sun S J, Jia Z C, Ma W, Mao C L, Ou C M, Wang J, Zhang H, Hong L, Li M L, Jia S G, Mao P S. Genome-wide analysis and expression profiling of glutathione reductase gene family in oat(Avena sativa)indicate their responses to abiotic stress during seed imbibition[J]. International Journal of Molecular Sciences, 2022, 23(19):11650.doi:10.3390/ijms231911650.
[13]	Canales F J, Montilla-Bascón G, Rispail N, Prats E. Salicylic acid regulates polyamine biosynthesis during drought responses in oat[J]. Plant Signaling & Behavior, 2019, 14(10):e1651183.doi:10.1080/15592324.2019.1651183.
[14]	Mi W B, Liu K Q, Liang G L, Jia Z F, Ma X, Ju Z L, Liu W H. Genome-wide identification and characterization of ABA receptor pyrabactin resistance 1-like protein(PYL)family in oat[J]. PeerJ, 2023,11:e16181.doi:10.7717/peerj.16181.
[15]	Naake T, Maeda H A, Proost S, Tohge T, Fernie A R. Kingdom-wide analysis of the evolution of the plant type Ⅲ polyketide synthase superfamily[J]. Plant Physiology, 2021, 185(3):857-875.doi:10.1093/plphys/kiaa086.
[16]	薛保国, 谢丽华, 杨丽荣, 孙润红, 全鑫, 杨艳艳, 孙虎, 雷振生, 赵献林. 小麦查尔酮合成酶基因及其启动子序列的克隆与分析[J]. 植物病理学报, 2017, 47(1):50-60.doi:10.13926/j.cnki.apps.000048.
	Xue B G, Xie L H, Yang L R, Sun R H, Quan X, Yang Y Y, Sun H, Lei Z S, Zhao X L. Cloning and functional analysis of TaCHS and its promoter in wheat[J]. Acta Phytopathologica Sinica, 2017, 47(1):50-60.
[17]	Król P, Igielski R, Pollmann S, Kępczyńska E. Priming of seeds with methyl jasmonate induced resistance to hemi-biotroph Fusarium oxysporum f.sp.lycopersici in tomato via 12-oxo-phytodienoic acid,salicylic acid,and flavonol accumulation[J]. Journal of Plant Physiology, 2015,179:122-132.doi:10.1016/j.jplph.2015.01.018.
[18]	Zuk M, Działo M, Richter D, Dymińska L, Matuła J, Kotecki A, Hanuza J, Szopa J. Chalcone synthase(CHS)gene suppression in flax leads to changes in wall synthesis and sensing genes,cell wall chemistry and stem morphology parameters[J]. Frontiers in Plant Science, 2016,7:894.doi:10.3389/fpls.2016.00894.
[19]	陈帅. 烟草类黄酮代谢途径中关键酶CHS基因与R2R3 MYB类转录抑制因子功能研究[D]. 雅安: 四川农业大学, 2017.
	Chen S. Study on the function of CHS gene,a key enzyme in tobacco flavonoid metabolism pathway,and R2R3 MYB transcription inhibitor[D]. Yaan: Sichuan Agricultural University, 2017.
[20]	Chen L J, Guo H M, Lin Y, Cheng H M. Chalcone synthase EaCHS1 from Eupatorium adenophorum functions in salt stress tolerance in tobacco[J]. Plant Cell Reports, 2015, 34(5):885-894.doi:10.1007/s00299-015-1751-7.
[21]	Hou Q D, Li S, Shang C Q, Wen Z, Cai X W, Hong Y, Qiao G. Genome-wide characterization of chalcone synthase genes in sweet cherry and functional characterization of CpCHS1 under drought stress[J]. Frontiers in Plant Science, 2022,13:989959.doi:10.3389/fpls.2022.989959.
[22]	梁先利, 张丽清, 柯丽萍, 孙玉强. 彩色棉查尔酮合成酶基因GhCHS1的克隆和功能分析[J]. 农业生物技术学报, 2019, 27(6):993-1002.doi:10.3969/j.issn.1674-7968.2019.06.005.
	Liang X L, Zhang L Q, Ke L P, Sun Y Q. Cloning of chalcone synthase gene GhCHS1 and its function analysis in coloured cotton(Gossypium hirsutum)[J]. Journal of Agricultural Biotechnology, 2019, 27(6):993-1002.
[23]	Tai D Q, Tian J, Zhang J, Song T T, Yao Y C. A Malus crabapple chalcone synthase gene,McCHS,regulates red petal color and flavonoid biosynthesis[J]. PLoS One, 2014, 9(10):e110570.doi:10.1371/journal.pone.0110570.
[24]	Wu X X, Zhang S M, Liu X H, Shang J, Zhang A D, Zhu Z W, Zha D S. Chalcone synthase(CHS)family members analysis from eggplant(Solanum melongena L.) in the flavonoid biosynthetic pathway and expression patterns in response to heat stress[J]. PLoS One, 2020, 15(4):e0226537.doi:10.1371/journal.pone.0226537.
[25]	纪瑞鹏, 于文颖, 冯锐, 武晋雯, 米娜, 张玉书. 骤发干旱对农作物生长过程的影响机制与评估研究进展[J]. 生态学杂志, 2023, 42(11):2737-2746.doi:10.13292/j.1000-4890.202311.008.
	Ji R P, Yu W Y, Feng R, Wu J W, Mi N, Zhang Y S. Research advances in the effect mechanism and evaluation of flash drought on crop growth process[J]. Chinese Journal of Ecology, 2023, 42(11):2737-2746.
[26]	Ma D Y, Sun D X, Wang C Y, Li Y G, Guo T C. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress[J]. Plant Physiology and Biochemistry, 2014,80:60-66.doi:10.1016/j.plaphy.2014.03.024.
[27]	Gao G R, Lyu Z R, Zhang G Y, Li J Y, Zhang J G, He C Y. An ABA flavonoid relationship contributes to the differences in drought resistance between different sea buckthorn subspecies[J]. Tree Physiology, 2021, 41(5):744-755.doi:10.1093/treephys/tpaa155.
[28]	Ghotbzadeh Kermani S, Saeidi G, Sabzalian M R, Gianinetti A. Drought stress influenced sesamin and sesamolin content and polyphenolic components in sesame(Sesamum indicum L.) populations with contrasting seed coat colors[J]. Food Chemistry, 2019,289:360-368.doi:10.1016/j.foodchem.2019.03.004.
[29]	Austin M B, Noel J P. The chalcone synthase superfamily of type Ⅲ polyketide synthases[J]. Natural Product Reports, 2003, 20(1):79-110.doi:10.1039/b100917f.
[30]	陈俊毅, 柴友荣, 姚永宏. 查尔酮合酶蛋白表达技术、结构、活性和进化[J]. 西南农业学报, 2012, 25(1):328-336.doi:10.16213/j.cnki.scjas.2012.01.002.
	Chen J Y, Chai Y R, Yao Y H. Protein expression technologies,structure,activity and evolution of chalcone synthase[J]. Southwest China Journal of Agricultural Sciences, 2012, 25(1):328-336.
[31]	刘素军, 蒙美莲, 陈有君. 干旱胁迫及复水对马铃薯类黄酮合成途径中关键酶及基因表达的影响[J]. 植物生理学报, 2018, 54(1):81-91.doi:10.13592/j.cnki.ppj.2017.0191.
	Liu S J, Meng M L, Chen Y J. Effects of drought stress and rehydration on gene expression of key enzymes in the flavonoid biosynthesis pathway in potato[J]. Plant Physiology Journal, 2018, 54(1):81-91.

引物名称 Primer name	引物序列(5'-3') Primer sequences(5'-3')	用途 Use
3448F	AGCAGCATCGTCTGTCATTC	gDNA克隆
3448R	GGTCAGTGGTCAGTCCTTTCT
3448Fc	AACACGGGGGACTTTGCAACatggccgcgaccatgaccg	cDNA克隆;载体构建
3448Rc	CCTGAAGCGGCCGCTGTACAagcggtggcaccagcggc
Glosgfp-F	tTCATTTGGAGAGAACACGGGggac	重组质粒鉴定
Glosgfp-R	gagtcgaggtgggcctcgt
3448Fq	TCCTAGATGAGATCCGTAAGCG	qPCR
3448Rq	AGGTCCAAATCCGAACAACA
Actin-F	GAGCGGGAAATTGTAAGGGAC	内参基因
Actin-R	ATGGATGGCTGGAAGAGGAC

引物名称 Primer name	引物序列(5'-3') Primer sequences(5'-3')	用途 Use
3448F	AGCAGCATCGTCTGTCATTC	gDNA克隆
3448R	GGTCAGTGGTCAGTCCTTTCT
3448Fc	AACACGGGGGACTTTGCAACatggccgcgaccatgaccg	cDNA克隆;载体构建
3448Rc	CCTGAAGCGGCCGCTGTACAagcggtggcaccagcggc
Glosgfp-F	tTCATTTGGAGAGAACACGGGggac	重组质粒鉴定
Glosgfp-R	gagtcgaggtgggcctcgt
3448Fq	TCCTAGATGAGATCCGTAAGCG	qPCR
3448Rq	AGGTCCAAATCCGAACAACA
Actin-F	GAGCGGGAAATTGTAAGGGAC	内参基因
Actin-R	ATGGATGGCTGGAAGAGGAC

软件 Software	网址 Website	用途 Use
GSDS 2.0	http://gsds.gao-lab.org/	基因结构分析
CD-Search	http://www.ncbi.nlm.gov/Structure/cdd/wrpsb.cgi	理化性质分析
SOPMA	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_sopma.html	二级结构预测
SWISS-MODEL	https://swissmodel.expasy.org/interactive	三级结构预测
TMHMMServer	https://services.healthtech.dtu.dk/service.php?TMHMM-2.0	跨膜结构域预测
Cell-PLoc 2.0	http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/	亚细胞定位
Jaiview		多重序列比对
MEGA 7.0		系统发育树构建

软件 Software	网址 Website	用途 Use
GSDS 2.0	http://gsds.gao-lab.org/	基因结构分析
CD-Search	http://www.ncbi.nlm.gov/Structure/cdd/wrpsb.cgi	理化性质分析
SOPMA	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=npsa_sopma.html	二级结构预测
SWISS-MODEL	https://swissmodel.expasy.org/interactive	三级结构预测
TMHMMServer	https://services.healthtech.dtu.dk/service.php?TMHMM-2.0	跨膜结构域预测
Cell-PLoc 2.0	http://www.csbio.sjtu.edu.cn/bioinf/plant-multi/	亚细胞定位
Jaiview		多重序列比对
MEGA 7.0		系统发育树构建

名称 Name	序列 Sequence	数量 Number	功能 Function
MBS	CAACTG	2	参与干旱胁迫的MYB结合位点
ARE	AAACCA	3	参与厌氧胁迫所必需的顺式调控元件
CAT-box	GCCACT	1	参与分生组织表达相关的顺式调控元件
ABRE	TACGGTC	3	脱落酸响应元件
CCAAT-box	CAACGG	2	参与昼夜节律调控的MYBHv1结合位点
CGTCA-motif	CGTCA	1	MeJA响应元件
G-box	CACGAC	1	光响应元件
GARE-motif	TCTGTTG	1	赤霉素响应元件
I-box	gGATAAGGTG	2	光响应元件
MRE	AACCTAA	1	光响应元件
SP1	GGGCGG	1	光响应元件

选择文件类型/文献管理软件名称

选择包含的内容

燕麦AsCHS基因克隆和表达分析

Cloning and Expression Analysis of AsCHS in Oat

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 31

相关文章 15

编辑推荐

Metrics

本文评价

[1]	殷冬冬, 朱梦琪, 兰梦蝶, 朱星星, 尹磊, 沈学怀, 王洁茹, 赵瑞宏, 戴银, 潘孝成. 禽腺病毒血清4型感染LMH细胞的环状RNA表达分析[J]. 华北农学报, 2025, 40(4): 219-224.
[2]	姜昊, 张琳杰, 蔡嘉茹, 王雪晴, 卢杰, 周毅, 朱玉磊, 王升星. 小麦HBD基因家族全基因组鉴定及功能分析[J]. 华北农学报, 2025, 40(4): 29-40.
[3]	李生梅, 李静, 庞博, 张茹, 耿世伟, 陈佳林, 宋武, 高文伟. *棉花微管蛋白Togaram1基因的克隆及表达分析*[J]. 华北农学报, 2025, 40(4): 76-84.
[4]	王子铭, 司吉浩, 张文治, 孟成, 王哲. 玉米IQM基因家族全基因组鉴定及胁迫表达分析[J]. 华北农学报, 2025, 40(4): 1-9.
[5]	张红梅, 杨海鹏, 刘娅娟, 龙芸, 张鹏安, 陈伟, 张杰, 侯凌鹏, 韩志玲, 刘小红. 玉米STAT转录因子家族成员基因的鉴定及其表达谱分析[J]. 华北农学报, 2025, 40(4): 10-18.
[6]	白宇捷, 赵然, 崔顺立, 侯名语, 李秀坤, 刘立峰, 刘盈茹. 不同基因型花生抗旱性分级及鉴定[J]. 华北农学报, 2025, 40(4): 107-118.
[7]	刘雅鑫, 陈涛, 高维东, 郭利建, 车卓, 杨德龙. 小麦E3泛素连接酶TaSINA101的抗逆相关功能研究[J]. 华北农学报, 2025, 40(4): 19-28.
[8]	蓝湘一, 何洋洋, 张明, 黄慈, 王海波, 王会, 柴志欣, 钟金城. *牦牛MAP3K7基因克隆及其与皮下前体脂肪细胞分化关系分析*[J]. 华北农学报, 2025, 40(3): 225-233.
[9]	胡雨莹, 孙茂, 汪骞, 黎志彬, 鲍锐, 桂敏, 钟秋月, 杜光辉, 吴丽艳. *野生蒜芥茄SsRPM1基因的生信分析、亚细胞定位及表达分析*[J]. 华北农学报, 2025, 40(3): 27-33.
[10]	冯清香, 朱哈修, 杜洋, 徐艳茹, 王晨晨, 刘雪, 李大勇, 周军, 张彬. *菊苣ANS基因的克隆及表达分析*[J]. 华北农学报, 2025, 40(3): 51-59.
[11]	王昭懿, 崔原瑗, 韩梦荞, 刘正文, 邓习, 豆飞飞, 任毓昭, 刘彩霞, 刘凤楼, 王掌军, 孙洋洋, 任民, 李清峰. 黑麦NHX基因家族的鉴定及盐胁迫下的表达分析[J]. 华北农学报, 2025, 40(3): 9-18.
[12]	高鹏华, 齐颖, 杨敏, 李丽芳, 黄飞燕, 刘佳妮, 赵建荣, 余磊. 感染白绢病对魔芋内源激素含量及相关基因表达的影响[J]. 华北农学报, 2025, 40(3): 149-156.
[13]	林世锋, 王仁刚, 王自力, 李莉, 陈越男, 吴沙沙, 刘国琴. 豆伞滑刃线虫类FMRF酰胺多肽基因的克隆与分析[J]. 华北农学报, 2025, 40(3): 166-172.
[14]	孔得文, 王维民, 田慧彬, 张德印, 赵利明, 杨晓斌, 马宗武, 李成海, 张健, 蒲萌茹, 曹佩亮, 李林庭, 李泓舰, 贾国星, 高飞, 武万恩, 王立忠, 冯炼君, 肖子越, 张琪, 闫成琪, 高磊, 张小雪. *湖羊FITM2基因多态性及其与生长性状的关联分析*[J]. 华北农学报, 2025, 40(3): 173-181.
[15]	王立琦, 张蕾, 李茜, 管圣, 卢建宁, 许明志, 崔国贤, 佘玮. *异源表达苎麻金属硫蛋白BnMLP2基因增强拟南芥对干旱胁迫的耐受性*[J]. 华北农学报, 2025, 40(3): 19-26.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

燕麦AsCHS基因克隆和表达分析

Cloning and Expression Analysis of AsCHS in Oat

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 31

相关文章 15

编辑推荐

Metrics

本文评价