玉米IQM基因家族全基因组鉴定及胁迫表达分析

doi:10.7668/hbnxb.20195645

摘要/Abstract

摘要：

IQM基因家族是Ca²⁺不依赖型钙调素结合蛋白中的重要成员,在植物生长发育以及多种胁迫反应中发挥着重要作用。为了研究玉米IQM基因家族的特征及潜在功能,采用生物信息学的方法在玉米全基因组中鉴定IQM基因,并对其蛋白性质、系统进化关系、基因结构、染色体位置、基因复制、顺式作用元件、组织特异性表达和多种胁迫下表达模式进行研究。结果表明,在玉米全基因组中共鉴定出11个ZmIQMs基因,根据其在染色体上的位置,将其命名为ZmIQM1~ZmIQM11。ZmIQMs基因分为3个亚家族,亚家族内部基因结构具有相似性,片段复制在玉米IQM基因家族扩增和进化中发挥主要作用。顺式作用元件分析发现,ZmIQMs基因启动子区含有多个与激素和胁迫响应相关的元件。对ZmIQMs基因的表达模式进行研究,发现ZmIQMs基因在不同组织中的表达模式不同,在不同非生物和生物胁迫下,多个ZmIQMs基因表达量发生变化。qRT-PCR结果显示,在干旱胁迫下,ZmIQM3、ZmIQM4和ZmIQM10上调表达;ZmIQM3、ZmIQM4、ZmIQM5、ZmIQM10和ZmIQM11响应小斑病病原菌的侵染。以上结果表明,ZmIQMs基因在胁迫响应中发挥着重要作用。

关键词: 玉米, IQM, 基因鉴定, 基因表达模式

Abstract:

IQM(IQ-motif containing protein),a plant-specific calmodulin-binding protein,plays crucial roles in plant growth,development,and responses to various stresses.In order to study the characteristics and potential functions of the maize IQM gene family,bioinformatics approaches were used to identify IQM genes in the maize whole genome,and protein properties,phylogenetic relationship,gene structure,chromosome location,gene replication,cis-acting element,tissue-specific expression and expression patterns under various stresses were investigated.A total of 11 ZmIQMs genes were identified in the whole genome of maize,named ZmIQM1 to ZmIQM11 based on their chromosomal locations.ZmIQMs genes could be classified into three subfamilies,with genes within different subfamilies exhibiting similar structures.Segment duplication was found to play a major role in the amplification and evolution of the ZmIQMs gene family.Cis-acting element analysis showed that the promoter region of ZmIQMs gene contained multiple hormone and stress response elements.The expression pattern of ZmIQMs genes was investigated,and it was found that ZmIQMs genes had different expression patterns in different tissues,and the expression levels of several ZmIQMs genes were changed under different abiotic and biotic stresses.qRT-PCR results showed that under drought stress,the expression of ZmIQM3,ZmIQM4 and ZmIQM10 was up-regulated, and ZmIQM3,ZmIQM4,ZmIQM5,ZmIQM10 and ZmIQM11 responded to Cochliobolus heterostrophus infection.The results showed that ZmIQMs genes played an important role in stress response.

Key words: Maize, IQM, Gene identification, Gene expression pattern

中图分类号:

S513.03

王子铭, 司吉浩, 张文治, 孟成, 王哲. 玉米IQM基因家族全基因组鉴定及胁迫表达分析[J]. 华北农学报, 2025, 40(4): 1-9. doi: 10.7668/hbnxb.20195645.

WANG Ziming, SI Jihao, ZHANG Wenzhi, MENG Cheng, WANG Zhe. Genome-wide Identification and Stress Expression Analysis of IQM Gene Family in Maize[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(4): 1-9. doi: 10.7668/hbnxb.20195645.

http://www.hbnxb.net/CN/Y2025/V40/I4/1

图/表 11

参考文献 31

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基因 Gene	上游引物(5'-3') Forward primer (5'-3')	下游引物(5'-3') Reverse primer (5'-3')
ZmIQM3	CAGAGAGGCGTATTCCAGCA	TGAGGAGGTTCTCTGCACTAGG
ZmIQM4	ACGGCAGGGACTTGCATCA	TACACGAGCCGCCCTTCCT
ZmIQM5	CATCATTAGCGAGGGAAAGGT	CAGTGAATCTCCCAGCAGCTA
ZmIQM10	AAGATGACGATGATGGCCAG	GTAGTGCAGGCTGGGCGA
ZmIQM11	GCCCGACGGAGGAGAACTT	TGCCCCAGAACTCCTCGTC
ZmEF-1α	TGGGCCTACTGGTCTTACTACTGA	ACATACCCACGCTTCAGATCCT

基因 Gene	上游引物(5'-3') Forward primer (5'-3')	下游引物(5'-3') Reverse primer (5'-3')
ZmIQM3	CAGAGAGGCGTATTCCAGCA	TGAGGAGGTTCTCTGCACTAGG
ZmIQM4	ACGGCAGGGACTTGCATCA	TACACGAGCCGCCCTTCCT
ZmIQM5	CATCATTAGCGAGGGAAAGGT	CAGTGAATCTCCCAGCAGCTA
ZmIQM10	AAGATGACGATGATGGCCAG	GTAGTGCAGGCTGGGCGA
ZmIQM11	GCCCGACGGAGGAGAACTT	TGCCCCAGAACTCCTCGTC
ZmEF-1α	TGGGCCTACTGGTCTTACTACTGA	ACATACCCACGCTTCAGATCCT

基因名称 Gene name	基因ID Gene ID	基因位置 Genome location	开放阅读框长度/bp Open reading frame length	氨基酸数/aa Amino acids	分子质量 /ku Molecular weight	等电点 Isoelectric point	染色体 Chromo- some	亚细胞定位 Subcellular localization
ZmIQM1	Zm00001d027815	136597264-136600860	1 929	642	71.583 58	8.94	1	细胞核
ZmIQM2	Zm00001d032981	223073135-223075832	1 971	656	73.426 83	8.39	1	细胞核
ZmIQM3	Zm00001d004916	79837610-79839037	1 104	367	41.307 62	8.89	2	细胞核
ZmIQM4	Zm00001d044253	192080883-192084967	1 689	562	62.140 52	9.50	3	叶绿体
ZmIQM5	Zm00001d052525	173537172-173539940	1 407	468	52.064 74	8.58	4	细胞核
ZmIQM6	Zm00001d035377	14329827-14333778	1 311	436	49.035 75	9.16	6	叶绿体
ZmIQM7	Zm00001d037759	23680168-23690640	1 587	528	58.222 00	9.36	6	细胞核
ZmIQM8	Zm00001d022245	154193631-154195926	1 986	661	73.130 90	7.54	7	细胞核
ZmIQM9	Zm00001d009755	123978579-123979350	1 341	446	49.004 68	9.59	8	细胞核
ZmIQM10	Zm00001d011228	84015105-84017555	1 653	550	60.230 04	9.43	8	叶绿体
ZmIQM11	Zm00001d047402	156642268-156645976	1 617	538	59.073 49	7.60	9	细胞核

基因名称 Gene name	基因ID Gene ID	基因位置 Genome location	开放阅读框长度/bp Open reading frame length	氨基酸数/aa Amino acids	分子质量 /ku Molecular weight	等电点 Isoelectric point	染色体 Chromo- some	亚细胞定位 Subcellular localization
ZmIQM1	Zm00001d027815	136597264-136600860	1 929	642	71.583 58	8.94	1	细胞核
ZmIQM2	Zm00001d032981	223073135-223075832	1 971	656	73.426 83	8.39	1	细胞核
ZmIQM3	Zm00001d004916	79837610-79839037	1 104	367	41.307 62	8.89	2	细胞核
ZmIQM4	Zm00001d044253	192080883-192084967	1 689	562	62.140 52	9.50	3	叶绿体
ZmIQM5	Zm00001d052525	173537172-173539940	1 407	468	52.064 74	8.58	4	细胞核
ZmIQM6	Zm00001d035377	14329827-14333778	1 311	436	49.035 75	9.16	6	叶绿体
ZmIQM7	Zm00001d037759	23680168-23690640	1 587	528	58.222 00	9.36	6	细胞核
ZmIQM8	Zm00001d022245	154193631-154195926	1 986	661	73.130 90	7.54	7	细胞核
ZmIQM9	Zm00001d009755	123978579-123979350	1 341	446	49.004 68	9.59	8	细胞核
ZmIQM10	Zm00001d011228	84015105-84017555	1 653	550	60.230 04	9.43	8	叶绿体
ZmIQM11	Zm00001d047402	156642268-156645976	1 617	538	59.073 49	7.60	9	细胞核

同源基因对 Paralogous pairs	复制类型 Duplicate type	非同义替换率 Ka	同义替换率 Ks	非同义替换率/同义替换率 Ka/Ks	复制时间/Mya Duplication time
ZmIQM1-ZmIQM2	片段重复	0.283 2	1.172 5	0.241 6	26.517 9
ZmIQM3-ZmIQM5	片段重复	0.098 5	0.346 7	0.283 9	9.414 2
ZmIQM4-ZmIQM10	片段重复	0.107 2	0.207 2	0.517 6	32.840 1
ZmIQM8-ZmIQM11	片段重复	0.203 4	0.689 9	0.294 8	26.150 2

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