湖羊<i>ANO5</i>基因多态性及其与脂肪沉积性状的关联分析

doi:10.7668/hbnxb.20194928

摘要/Abstract

摘要：

茴香胺5(ANO5)是一种定位于肌膜和肌浆网的多通道膜蛋白,主要在肌膜修复和磷脂争夺中起作用,ANO5基因突变会导致颌骨发育不全以及各种肌肉疾病。为了探讨绵羊ANO5基因的单核苷酸多态性与脂肪沉积性状的关联性,选择健康且系谱清晰的1 005只湖羊公羔群体为研究对象,采用PCR扩增技术和KASPar分型技术对试验群体ANO5基因位点多态性进行检测,并与脂肪沉积性状进行关联分析,并利用qPCR分析ANO5基因在不同组织中的表达水平。结果表明,绵羊ANO5基因在湖羊多种组织中广泛表达,与其他组织相比,ANO5基因在心脏组织中的表达量最高。在绵羊ANO5基因第10内含子中检测到了g.58010 C>T多态位点的3种基因型CC、CT和TT。描述性统计结果表明,肾周脂肪质量与其他脂肪质量的差异最大,变异程度最高。相关分析结果表明,脂肪沉积相关性状与生长性状、饲料效率性状均呈正相关。关联分析结果表明,该多态位点与湖羊肾周脂肪质量及其相关性状呈显著关联。其中,CC基因型个体的肾周脂肪质量及其相对质量均显著低于TT基因型个体。综上所述,绵羊ANO5基因g.58010 C>T突变位点可作为湖羊肾周脂肪沉积性状的候选分子标记。

关键词: 湖羊, 茴香胺5, 多态性, 肾周脂肪, 关联分析

Abstract:

Anoctamin 5(ANO5)is a multichannel membrane protein localized in the sarcoplasmic and sarcoplasmic reticulum that primarily plays a role in myosin membrane repair and phospholipid scrambling,mutations in the ANO5 gene can lead to jaw hypoplasia as well as various myopathies.It aimed to investigate the association of SNPs in the ANO5 gene with fat deposition traits in sheep.A population of 1 005 healthy and clearly genealogical Hu sheep male lambs was selected for the study,and PCR amplification and KASPar typing techniques were used to detect the locus polymorphisms of the ANO5 gene in the experimental population and analyze the associations with fat deposition traits.The expression level of ANO5 gene in different tissues was analyzed by qPCR.The results showed that sheep ANO5 gene was widely expressed in a variety of tissues in Hu sheep,and the highest expression of ANO5 gene was found in heart tissue compared with other tissues.Three genotypes of CC,CT and TT with the g.58010 C>T polymorphic locus were detected in the 10th intron of the sheep ANO5 gene.Descriptive statistics showed that the perirenal fat weight was the most different and had the highest degree of variability compared with other fat weights.Correlation analysis showed that fat deposition related traits were positively correlated with growth and feed efficiency traits,and the results of association analyses showed that the polymorphic locus was significantly associated with perirenal fat weight and its related traits in the Hu sheep.Among them,the perirenal fat weight of individuals with CC genotype was significantly lower than that of individuals with TT genotype.In conclusion,the g.58010 C>T mutation locus of the ANO5 gene can be used as a candidate molecular marker for perirenal fat deposition traits in Hu sheep.

Key words: Hu sheep, Anoctamin 5(ANO5), Polymorphism, Perirenal fat, Association analysis

黄志强, 王维民, 张德印, 赵源, 张煜坤, 徐丹, 杨晓斌, 马宗武, 何丽娟, 蔡有鑫, 刘晓强, 张小雪. 湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析[J]. 华北农学报, 2025, 40(1): 207-214. doi: 10.7668/hbnxb.20194928.

HUANG Zhiqiang, WANG Weimin, ZHANG Deyin, ZHAO Yuan, ZHANG Yukun, XU Dan, YANG Xiaobin, MA Zongwu, HE Lijuan, CAI Youxin, LIU Xiaoqiang, ZHANG Xiaoxue. Analysis of ANO5 Gene Polymorphism and Its Association with Fat Deposition Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 207-214. doi: 10.7668/hbnxb.20194928.

http://www.hbnxb.net/CN/Y2025/V40/I1/207

图/表 10

参考文献 38

[1]	Ma Z W, Wang W M, Zhang D Y, Zhang Y K, Zhao Y, Li X L, Zhao L M, Lin C C, Wang J H, Zhou B B, Cheng J B, Xu D, Li W X, Yang X B, Huang Y L, Cui P P, Liu J, Zeng X W, Zhai R, Zhang X X. Ovine RAP1GAP and rBAT gene polymorphisms and their association with tail fat deposition in Hu sheep[J]. Frontiers in Veterinary Science, 2022, 9:974513.doi:10.3389/fvets.2022.974513.
[2]	白飞英, 李建明, 王媛, 闫振富. 湖羊及其利用[J]. 北方牧业, 2021(4):12-13.
	Bai F Y, Li J M, Wang Y, Yan Z F. Hu sheep and its utilization[J]. Northern Pastoralism, 2021(4):12-13.
[3]	杨雪, 覃圣, 王慧, 千志松, 戴珍珍, 彭瑜雪, 马小琴, 蔡勇. 藏羊与小尾寒羊不同部位脂肪组织特性对比[J]. 现代畜牧兽医, 2022(2):32-35.doi:10.3969/j.issn.1672-9692.2022.2.lnxmsy202202008.
	Yang X, Qin S, Wang H, Qian Z S, Dai Z Z, Peng Y X, Ma X Q, Cai Y. Comparison of adipose tissue characteristics in different parts of Tibetan sheep and Small-Tailed Han sheep[J]. Modern Journal of Animal Husbandry and Veterinary Medicine, 2022(2):32-35.
[4]	闫雪, 孙爱丽. 肾周脂肪在糖尿病肾病中的作用及机制研究进展[J]. 老年医学研究, 2022, 3(6):38-42.doi:10.3969/j.issn.2096-9058.2022.06.009.
	Yan X, Sun A L. Research progress on the role and mechanism of perirenal adipose tissue in diabetic nephropathy[J]. Geriatrics Research, 2022, 3(6):38-42.
[5]	Zhang D Y, Zhang X X, Li F D, La Y F, Li G Z, Zhang Y K, Li X L, Zhao Y, Song Q Z, Wang W M. The association of polymorphisms in the ovine PPARGC1B and ZEB2 genes with body weight in Hu sheep[J]. Animal Biotechnology, 2022, 33(1):90-97.doi:10.1080/10495398.2020.1775626.
[6]	Hartzell H C, Yu K, Xiao Q H, Chien L T, Qu Z Q. Anoctamin/TMEM16 family members are Ca²⁺-activated Cl^- channels[J]. The Journal of Physiology, 2009, 587(Pt 10):2127-2139.doi:10.1113/jphysiol.2008.163709. pmid: 19015192
[7]	Griffin D A, Johnson R W, Whitlock J M, Pozsgai E R, Heller K N, Grose W E, Arnold W D, Sahenk Z, Hartzell H C, Rodino-Klapac L R. Defective membrane fusion and repair in anoctamin5-deficient muscular dystrophy[J]. Human Molecular Genetics, 2016, 25(10):1900-1911.doi:10.1093/hmg/ddw063. pmid: 26911675
[8]	Benarroch E E. Anoctamins(TMEM16 proteins):functions and involvement in neurologic disease[J]. Neurology, 2017, 89(7):722-729.doi:10.1212/WNL.0000000000004246. pmid: 28724583
[9]	Chandra G, Defour A, Mamchoui K, Pandey K, Mishra S, Mouly V, Sreetama S, Mahad Ahmad M, Mahjneh I, Morizono H, Pattabiraman N, Menon A K, Jaiswal J K. Dysregulated calcium homeostasis prevents plasma membrane repair in anoctamin 5/TMEM16E-deficient patient muscle cells[J]. Cell Death Discovery, 2019, 5:118.doi:10.1038/s41420-019-0197-z. pmid: 31341644
[10]	Pedemonte N, Galietta L J V. Structure and function of TMEM16 proteins(anoctamins)[J]. Physiological Reviews, 2014, 94(2):419-459.doi:10.1152/physrev.00039.2011.
[11]	Tsutsumi S, Kamata N, Vokes T J, Maruoka Y, Nakakuki K, Enomoto S, Omura K, Amagasa T, Nagayama M, Saito-Ohara F, Inazawa J, Moritani M, Yamaoka T, Inoue H, Itakura M. The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia(GDD)[J]. The American Journal of Human Genetics, 2004, 74(6):1255-1261.doi:10.1086/421527.
[12]	de Bruyn A, Montagnese F, Holm-Yildiz S, Scharff Poulsen N, Stojkovic T, Behin A, Palmio J, Jokela M, De Bleecker J L, de Visser M, van der Kooi A J, Ten Dam L, Domínguez González C, Maggi L, Gallone A, Kostera-Pruszczyk A, Macias A, Łusakowska A, Nedkova V, Olive M, Álvarez-Velasco R, Wanschitz J, Paradas C, Mavillard F, Querin G, Fernández-Eulate G, Quinlivan R, Walter M C, Depuydt C E, Udd B, Vissing J, Schoser B, Claeys K G. Anoctamin-5 related muscle disease:clinical and genetic findings in a large European cohort[J]. Brain, 2023, 146(9):3800-3815.doi:10.1093/brain/awad088.
[13]	Sui T T, Xu L, Lau Y S, Liu D, Liu T J, Gao Y D, Lai L X, Han R Z, Li Z J. Development of muscular dystrophy in a CRISPR-engineered mutant rabbit model with frame-disrupting ANO5 mutations[J]. Cell Death & Disease, 2018, 9(6):609.doi:10.1038/s41419-018-0674-y.
[14]	Wosczyna M N, Perez Carbajal E E, Wagner M W, Paredes S, Konishi C T, Liu L, Wang T T, Walsh R A, Gan Q, Morrissey C S, Rando T A. Targeting microRNA-mediated gene repression limits adipogenic conversion of skeletal muscle mesenchymal stromal cells[J]. Cell Stem Cell, 2021, 28(7):1323-1334.e8.doi:10.1016/j.stem.2021.04.008. pmid: 33945794
[15]	Wosczyna M N, Konishi C T, Perez Carbajal E E, Wang T T, Walsh R A, Gan Q, Wagner M W, Rando T A. Mesenchymal stromal cells are required for regeneration and homeostatic maintenance of skeletal muscle[J]. Cell Reports, 2019, 27(7):2029-2035.e5.doi:10.1016/j.celrep.2019.04.074. pmid: 31091443
[16]	He C L, Holme J, Anthony J. SNP genotyping:the KASP assay[J]. Methods in Molecular Biology, 2014, 1145:75-86.doi:10.1007/978-1-4939-0446-4_7.
[17]	孙国权, 高树新, 吴慧光, 李俊雅, 丽春, 王景山, 王玉泉, 王国富. 解偶联蛋白1、2和3基因在中国西门塔尔牛组织器官中的表达水平及其与胴体品质关系分析[J]. 华北农学报, 2014, 29(4):116-120.doi:10.7668/hbnxb.2014.04.019.
	Sun G Q, Gao S X, Wu H G, Li J Y, Li C, Wang J S, Wang Y Q, Wang G F. Analyze the ucp1,ucp2,ucp3 genes expression level in tissues/organs and the relationship with the carcass traits in Chinese simmental cattle[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(4):116-120.
[18]	Zhao H Y, Wu X F, Cai H F, Pan C Y, Lei C Z, Chen H, Lan X Y. Genetic variants and effects on milk traits of the caprine paired-like homeodomain transcription factor 2(PITX2)gene in dairy goats[J]. Gene, 2013, 532(2):203-210.doi:10.1016/j.gene.2013.09.062.
[19]	张圆, 吴利锋, 魏波, 李明宝, 吴付安, 尤培国, 商爱国, 王伟. 湖羊饲养及繁育管理要点[J]. 中国畜禽种业, 2020, 16(11):98-99.doi:10.3969/j.issn.1673-4556.2020.11.066.
	Zhang Y, Wu L F, Wei B, Li M B, Wu F A, You P G, Shang A G, Wang W. Key points of feeding and breeding management of Hu sheep[J]. The Chinese Livestock and Poultry Breeding, 2020, 16(11):98-99.
[20]	Cui P P, Wang W M, Zhang D Y, Li C, Huang Y L, Ma Z W, Wang X J, Zhao L M, Zhang Y K, Yang X B, Xu D, Cheng J B, Li X L, Zeng X W, Zhao Y, Li W X, Wang J H, Lin C C, Zhou B B, Liu J, Zhai R, Zhang X X. Identification of TRAPPC9 and BAIAP2 gene polymorphisms and their association with fat deposition-related traits in Hu sheep[J]. Frontiers in Veterinary Science, 2022, 9:928375.doi:10.3389/fvets.2022.928375.
[21]	Zhang R F, Li X F. Association between IGF-IR,m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattle[J]. Molecular Biology Reports, 2011, 38(3):2179-2184.doi:10.1007/s11033-010-0346-1.
[22]	Juszczuk-Kubiak E, Bujko K, Grzes' M, Cymer M, Wicińska K, Szostak A, Pierzchała M. Study of bovine Mef2B gene:the temporal-spatial expression patterns,polymorphism and association analysis with meat production traits[J]. Journal of Animal Science, 2016, 94(11):4536-4548.doi:10.2527/jas.2016-0741. pmid: 27898947
[23]	Sherman E L, Nkrumah J D, Murdoch B M, Li C, Wang Z, Fu A, Moore S S. Polymorphisms and haplotypes in the bovine neuropeptide Y,growth hormone receptor,ghrelin,insulin-like growth factor 2,and uncoupling proteins 2 and 3 genes and their associations with measures of growth,performance,feed efficiency,and carcass merit in beef cattle[J]. Journal of Animal Science, 2008, 86(1):1-16.doi:10.2527/jas.2006-799. pmid: 17785604
[24]	Cheng J B, Zhang X X, Li F D, Yuan L F, Zhang D Y, Zhang Y K, Song Q Z, Li X L, Zhao Y, Xu D, Zhao L M, Li W X, Wang J H, Zhou B B, Lin C C, Yang X B, Wang W M. Detecting single nucleotide polymorphisms in MEF2B and UCP3 and elucidating their association with sheep growth traits[J]. DNA and Cell Biology, 2021, 40(12):1554-1562.doi:10.1089/dna.2021.0782.
[25]	Walsh I M, Bowman M A, Soto Santarriaga I F, Rodriguez A, Clark P L. Synonymous codon substitutions perturb cotranslational protein folding in vivo and impair cell fitness[J]. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(7):3528-3534.doi:10.1073/pnas.1907126117. pmid: 32015130
[26]	Wang W, Huang R, Tang P T, Tu M, Guo X L. Perirenalfat thickness is associated with bone turnover markers and bone mineral density in postmenopausal women with type 2 diabetes mellitus[J]. Frontiers in Endocrinology, 2022, 13:990667.doi:10.3389/fendo.2022.990667.
[27]	Fang L L, Guo F J, Zhou L H, Stahl R, Grams J. The cell size and distribution of adipocytes from subcutaneous and visceral fat is associated with type 2 diabetes mellitus in humans[J]. Adipocyte, 2015, 4(4):273-279.doi:10.1080/21623945.2015.1034920. pmid: 26451283
[28]	Foster M C, Hwang S J, Porter S A, Massaro J M, Hoffmann U, Fox C S. Fatty kidney,hypertension,and chronic kidney disease:the framingham heart study[J]. Hypertension, 2011, 58(5):784-790.doi:10.1161/HYPERTENSIONAHA.111.175315.
[29]	Bredella M A, Torriani M, Ghomi R H, Thomas B J, Brick D J, Gerweck A V, Harrington L M, Breggia A, Rosen C J, Miller K K. Determinants of bone mineral density in obese premenopausal women[J]. Bone, 2011, 48(4):748-754.doi:10.1016/j.bone.2010.12.011. pmid: 21195217
[30]	Choi H S, Kim K J, Kim K M, Hur N W, Rhee Y, Han D S, Lee E J, Lim S K. Relationship between visceral adiposity and bone mineral density in Korean adults[J]. Calcified Tissue International, 2010, 87(3):218-225.doi:10.1007/s00223-010-9398-4. pmid: 20631995
[31]	Kim J H, Han E H, Jin Z W, Lee H K, Fujimiya M, Murakami G, Cho B H. Fetal topographical anatomy of the upper abdominal lymphatics:its specific features in comparison with other abdominopelvic regions[J]. The Anatomical Record, 2012, 295(1):91-104.doi:10.1002/ar.21527.
[32]	Wang Q A, Tao C, Jiang L, Shao M L, Ye R S, Zhu Y, Gordillo R, Ali A, Lian Y, Holland W L, Gupta R K, Scherer P E. Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation[J]. Nature Cell Biology, 2015, 17(9):1099-1111.doi:10.1038/ncb3217. pmid: 26280538
[33]	Hausman G J. Anatomical and enzyme histochemical differentiation of adipose tissue[J]. International Journal of Obesity, 1985, 9(S1):1-6.
[34]	周月, 李萌, 左莹. 肾周脂肪组织与心血管疾病的研究进展[J]. 现代医药卫生, 2023, 39(4):668-672.doi:10.3969/j.issn.1009-5519.2023.04.028.
	Zhou Y, Li M, Zuo Y. Research progress of perirenal adipose tissue and cardiovascular diseases[J]. Journal of Modern Medicine & Health, 2023, 39(4):668-672.
[35]	Penttilä S, Palmio J, Suominen T, Raheem O, Evilä A, Muelas Gomez N, Tasca G, Waddell L B, Clarke N F, Barboi A, Hackman P, Udd B. Eight new mutations and the expanding phenotype variability in muscular dystrophy caused by ANO5[J]. Neurology, 2012, 78(12):897-903.doi:10.1212/WNL.0b013e31824c4682. pmid: 22402862
[36]	Schroeder B C, Cheng T, Jan Y N, Jan L Y. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit[J]. Cell, 2008, 134(6):1019-1029.doi:10.1016/j.cell.2008.09.003. pmid: 18805094
[37]	Almaça J, Tian Y M, Aldehni F, Ousingsawat J, Kongsuphol P, Rock J R, Harfe B D, Schreiber R, Kunzelmann K. TMEM16 proteins produce volume-regulated chloride currents that are reduced in mice lacking TMEM16A[J]. Journal of Biological Chemistry, 2009, 284(42):28571-28578.doi:10.1074/jbc.M109.010074. pmid: 19654323
[38]	Mizuta K, Tsutsumi S, Inoue H, Sakamoto Y, Miyatake K, Miyawaki K, Noji S, Kamata N, Itakura M. Molecular characterization of GDD1/TMEM16E,the gene product responsible for autosomal dominant gnathodiaphyseal dysplasia[J]. Biochemical and Biophysical Research Communications, 2007, 357(1):126-132.doi:10.1016/j.bbrc.2007.03.108.

引物名称 Primer name	引物序列(5'—3') Primer sequences (5'—3')	产物大小/bp Size	最适温度/℃ TM
ANO5-PCR	F: GTGCTCCTTCATTATCCTT	710	46.3
	R: CAGAACAACTGCCTTCACA
ANO5-qPCR	F: AACCCCTTTTAGTTGTATGCTT	202	64.0
	R: ATCCTCCATGCCAAAAGGACA
UXT-qPCR	F: GCAAGTGGATTTGGGCTGTAAC	180	59.0
	R: ATGGAGTCCTTGGTGAGGCTGT

引物名称 Primer name	引物序列(5'—3') Primer sequences (5'—3')	产物大小/bp Size	最适温度/℃ TM
ANO5-PCR	F: GTGCTCCTTCATTATCCTT	710	46.3
	R: CAGAACAACTGCCTTCACA
ANO5-qPCR	F: AACCCCTTTTAGTTGTATGCTT	202	64.0
	R: ATCCTCCATGCCAAAAGGACA
UXT-qPCR	F: GCAAGTGGATTTGGGCTGTAAC	180	59.0
	R: ATGGAGTCCTTGGTGAGGCTGT

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
ANO5-Ra	GAAGGTGACCAAGTTCATGCTATCAAGTTTTAATCAGTTTGAATTTGTA
ANO5-Rg	GAAGGTCGGAGTCAACGGATTTCAAGTTTTAATCAGTTTGAATTTGTG
ANO5-F	GCCTTTTAATTTGATTAGGTAAGTTTC

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')
ANO5-Ra	GAAGGTGACCAAGTTCATGCTATCAAGTTTTAATCAGTTTGAATTTGTA
ANO5-Rg	GAAGGTCGGAGTCAACGGATTTCAAGTTTTAATCAGTTTGAATTTGTG
ANO5-F	GCCTTTTAATTTGATTAGGTAAGTTTC

项目 Items	最大值 Max	最小值 Min	均值 Mean	标准差 s	变异系数/% CV
尾脂质量/kg The weight of tail fat	2.850	0.000	1.121	0.467	41.66
尾脂相对质量(体质量) The relative weight of tail fat (Body weight)	0.057	0.000	0.025	0.008	32.08
尾脂相对质量(胴体质量) The relative weight of tail fat (Carcass weight)	0.106	0.000	0.046	0.014	30.42
肾周脂肪质量/kg The weight of perirenal fat	6.850	0.005	0.562	0.439	78.11
肾周脂肪相对质量(体质量) The relative weight of perirenal fat (Body weight)	0.141	0.000	0.012	0.008	67.62
肾周脂肪相对质量(胴体质量) The relative weight of perirenal fat (Carcass weight)	0.254	0.001	0.022	0.014	65.27
肠系膜脂肪质量/kg The weight of mesenteric fat	3.060	0.005	0.877	0.488	55.67
肠系膜脂肪相对质量(体质量) The relative weight of mesenteric fat (Body weight)	0.051	0.001	0.019	0.008	44.20
肠系膜脂肪相对质量(胴体质量) The relative weight of mesenteric fat (Carcass weight)	0.092	0.001	0.035	0.015	42.47

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湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析

Analysis of ANO5 Gene Polymorphism and Its Association with Fat Deposition Traits in Hu Sheep

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项目 Traits	ANO5 g.58010 C>T
项目 Traits	CC		CT		TT
个体数Number of individuals	663	251		19
尾脂质量/kg The weight of tail fat	1.114±0.446a		1.139±0.504a		1.115±0.660a
尾脂相对质量(体质量) The relative weight of tail fat (Body weight)	0.025±0.007a		0.025±0.009a		0.023±0.011a
尾脂相对质量(胴体质量) The relative weight of tail fat (Carcass weight)	0.046±0.013a		0.046±0.015a		0.043±0.018a
肾周脂肪质量/kg The weight of perirenal fat	0.546±0.443b		0.593±0.422ab		0.726±0.521a
肾周脂肪相对质量(体质量) The relative weight of perirenal fat (Body weight)	0.012±0.008b		0.012±0.007ab		0.015±0.009a
肾周脂肪相对质量(胴体质量) The relative weight of perirenal fat (Carcass weight)	0.021±0.015b		0.023±0.013ab		0.027±0.016a
肠系膜脂肪质量/kg The weight of mesenteric fat	0.868±0.486a		0.885±0.481a		0.961±0.609a
肠系膜脂肪相对质量(体质量) The relative weight of mesenteric fat (Body weight)	0.019±0.008a		0.019±0.008a		0.020±0.010a
肠系膜脂肪相对质量(胴体质量) The relative weight of mesenteric fat (Carcass weight)	0.035±0.015a		0.035±0.014a		0.036±0.018a

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基因型 Genotype	个数 No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	Ne	Ho	He	PIC
TT	19	0.02	T	0.16	0.73	0.27	1.37	0.23
CT	251	0.27
CC	663	0.71	C	0.84

基因型 Genotype	个数 No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	Ne	Ho	He	PIC
TT	19	0.02	T	0.16	0.73	0.27	1.37	0.23
CT	251	0.27
CC	663	0.71	C	0.84

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湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析

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