低温胁迫对普通越冬栽培稻幼苗耐寒生理生化指标的影响

doi:10.7668/hbnxb.20193901

摘要/Abstract

摘要：

研究不同越冬栽培稻种质资源的耐寒生理生化特性,为越冬栽培稻种质资源的创新利用提供理论依据。以20份普通越冬栽培稻种质为试验材料,测定并分析了常温(25 ℃)和低温(4 ℃)胁迫4 d后幼苗的叶绿素、丙二醛和SOD活性等5个耐寒生理生化指标。结果表明,正常生境下,20份普通越冬栽培稻种质幼苗期5个耐寒指标表现出不同程度的极显著差异。低温胁迫后,20份供试材料幼苗的5个耐寒指标发生了显著变化、同样表现出不同程度的极显著差异;各供试材料幼苗叶绿素和可溶性糖含量低温胁迫值较常温值均表现出显著的下降趋势,且它们的下降幅度不一致,分别为-36.46%~-90.31%,-70.54%~-94.03%。然而,丙二醛、脯氨酸和SOD活性3个耐寒指标低温胁迫值较常温值升降趋势不一致。其中,黄糯2号、晚粳糯M99037和常农粳3号丙二醛含量低温胁迫值较常温值显著降低,其余17份材料丙二醛含量值较常温值显著上升变化;铁粳稻4号、镇稻7号和津稻1187等8份材料脯氨酸含量低温胁迫值较常温值呈上升变化,其余12份材料较常温值呈下降变化。鄂晚稻15和津粳杂四幼苗SOD活性低温胁迫值较常温值略微下降、其余18份材料幼苗SOD活性值较常温值显著上升。正常生境下,各供试材料5个耐寒指标间仅检测到2对极显著相关性,低温胁迫后却检测到8对极显著相关,低温胁迫影响耐寒指标间相关性的数量、强度和方向。综合来看,无论在常温和低温胁迫后,20份普通越冬栽培稻幼苗5个耐寒指标因品种间遗传背景差异而表现出显著差异。

关键词: 越冬栽培稻, 低温胁迫, 生理生化指标

Abstract:

The physiological and biochemical indices of cold tolerance in common overwintering cultivated rice seedling were studied for providing theoretical basis for the innovation and utilization of overwintering cultivated rice germplasm. A total of 20 overwintering cultivated rice germplasms were selected as experimental materials,altogether five physiological and biochemical indices such as chlorophyll,malondialdehyde and SOD activity in rice seedling were measured and analyzed under both normal temperature(25 ℃)and 4 days of low temperature(4 ℃)stress. The five cold tolerance indices under normal temperature condition displayed extremely significant phenotypic differences among all tested materials from seven different provinces in China. The cold tolerance index such as chlorophyll and soluble sugar content apparently declined after chilling stress,but exhibited an inconsistent rate of decline among all experimental materials,ranged from -36.46% to -90.31%,-70.54% to -94.03%,respectively. While the cold tolerance indices such as malondialdehyde,proline and SOD activity in all tested rice seedlings displayed disparate response to cold chilling and exhibited an inconsistent rate of rising or falling. Among all tested materials,the malondialdehyde content in three tested materials such as Huangnuo 2,Wanjingnuo M99037 and Changnongjing 3 exhibited significantly decline after chilling stress,the remaining seventeen tested materials exhibited a significant rise. The proline content in eight tested materials such as Tiejingdao 4,Zhendao 7 and Jindao 1187 exhibited a significantly rising after being chilling stress,but the remaining twelve tested materials exhibited a significant decline malondialdehyde content value. The SOD activity in Ewandao 15 and Jinjingzasi exhibited an inconspicuous decline after chilling stress,while the SOD activity in the remaining eighteen tested materials exhibiteda significant rise. Only 2 pairs of extremely significant PCC values among the five cold tolerance indices were calculated under the normal temperature environment,while 8 pairs of extremely significant PCC values were calculated after chilling stress. Apparently,the number,strength and direction of correlations among the 5 cold tolerance indices were easily varied after chilling stress. On the whole,the cold tolerance indices in rice seedling under both normal temperature and low temperature exhibited significant difference owing to the different genetic background of overwintering cultivated rice.

Key words: Overwintering cultivated rice, Chilling stress, Physiological and biochemical index

严雨欣, 龚俊义, 彭廷燊, 秦小健, 南文斌, 张汉马, 梁永书. 低温胁迫对普通越冬栽培稻幼苗耐寒生理生化指标的影响[J]. 华北农学报, 2023, 38(S1): 67-77. doi: 10.7668/hbnxb.20193901.

YAN Yuxin, GONG Junyi, PENG Tingshen, QIN Xiaojian, NAN Wenbin, ZHANG Hanma, LIANG Yongshu. Effect of Low Temperature Stress on Physiological and Biochemical Indices of Cold Tolerance in Common Overwintering Cultivated Rice Seedling[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 67-77. doi: 10.7668/hbnxb.20193901.

http://www.hbnxb.net/CN/Y2023/V38/IS1/67

图/表 9

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编号 No.	供试材料 Tested material	省份 Province
1	浙糯36	浙江
2	秀水52	浙江
3	鄂晚稻15	湖北
4	鄂晚稻13	湖北
5	皖稻90	安徽
6	黄糯2号	安徽
7	晚粳糯M99037	安徽
8	常农粳3号	江苏
9	苏香粳2号	江苏
10	苏粳8号	江苏
11	镇稻7号	江苏
12	津稻1187	天津
13	津优2006	天津
14	津原5号	天津
15	津粳杂四	天津
16	铁粳稻4号	辽宁
17	辽盐稻241	辽宁
18	沈稻3号	辽宁
19	长白7号	吉林
20	嘉禾218	浙江

编号 No.	供试材料 Tested material	省份 Province
1	浙糯36	浙江
2	秀水52	浙江
3	鄂晚稻15	湖北
4	鄂晚稻13	湖北
5	皖稻90	安徽
6	黄糯2号	安徽
7	晚粳糯M99037	安徽
8	常农粳3号	江苏
9	苏香粳2号	江苏
10	苏粳8号	江苏
11	镇稻7号	江苏
12	津稻1187	天津
13	津优2006	天津
14	津原5号	天津
15	津粳杂四	天津
16	铁粳稻4号	辽宁
17	辽盐稻241	辽宁
18	沈稻3号	辽宁
19	长白7号	吉林
20	嘉禾218	浙江

供试材料 Tested materials	叶绿素含量/(μg/g) Chlorophyll content		变化比例/% Change ratio	T测验值 T-test value
供试材料 Tested materials	正常25 ℃ Normal temperature	低温4 ℃ Low temperature	变化比例/% Change ratio	T测验值 T-test value
浙糯36 Zhenuo 36	260.69±1.90g	165.65±0.03c	-36.46	111.95^**
秀水52 Xiushui 52	247.48±2.65h	99.13±0.03d	-59.95	125.25^**
鄂晚稻15 Ewandao 15	182.15±3.53m	51.28±0.02n	-71.85	83.24^**
鄂晚稻13 Ewandao 13	274.97±3.50ef	26.62±0.03s	-90.31	157.20^**
皖稻90 Wandao 90	354.60±4.20b	65.31±0.02i	-81.58	154.40^**
黄糯2号Huangnuo 2	248.39±2.15h	62.61±0.03l	-74.8	192.14^**
晚粳糯M99037 Wanjingnuo M99037	327.44±2.06c	97.55±0.03e	-70.21	250.57^**
常农粳3号Changnongjing 3	194.49±1.80k	28.50±0.02r	-85.35	208.96^**
苏香粳2号Suxiangjing 2	258.70±1.16g	34.66±0.03q	-86.6	423.73^**
苏粳8号Sujing 8	157.20±1.78n	9.53±0.02t	-93.94	186.96^**
镇稻7号Zhendao 7	400.67±2.33a	201.84±0.03a	-49.62	192.40^**
津稻1187 Jindao 1187	187.43±2.97l	64.61±0.02j	-65.53	92.43^**
津优2006 Jinyou 2006	269.46±3.27f	88.22±0.02f	-67.26	123.72^**
津原5号Jinyuan 5	277.69±5.30e	64.41±0.04k	-76.81	90.34^**
津粳杂四Jinjingzasi	218.60±4.41j	78.51±0.03h	-64.08	71.37^**
铁粳稻4号Tiejingdao 4	242.66±2.29h	50.19±0.03p	-79.32	186.06^**
辽盐稻241 Liaoyandao 241	228.63±3.49i	57.48±0.05m	-74.86	109.24^**
沈稻3号Shendao 3	215.76±3.17j	84.50±0.04g	-60.83	92.78**
长白7号Changbai 7	198.72±4.09k	50.52±0.04o	-74.56	80.10**
嘉禾218 Jiahe 218	318.70±3.80d	193.82±0.05b	-49.22	72.92**
平均值±标准差Means±s	253.22±59.85	78.75±51.22	-70.66±14.22	150.29±80.88
变化范围Range	157.20~400.67	9.53~201.84	-36.46~-93.94	71.37~423.73

供试材料 Tested materials	叶绿素含量/(μg/g) Chlorophyll content		变化比例/% Change ratio	T测验值 T-test value
供试材料 Tested materials	正常25 ℃ Normal temperature	低温4 ℃ Low temperature	变化比例/% Change ratio	T测验值 T-test value
浙糯36 Zhenuo 36	260.69±1.90g	165.65±0.03c	-36.46	111.95^**
秀水52 Xiushui 52	247.48±2.65h	99.13±0.03d	-59.95	125.25^**
鄂晚稻15 Ewandao 15	182.15±3.53m	51.28±0.02n	-71.85	83.24^**
鄂晚稻13 Ewandao 13	274.97±3.50ef	26.62±0.03s	-90.31	157.20^**
皖稻90 Wandao 90	354.60±4.20b	65.31±0.02i	-81.58	154.40^**
黄糯2号Huangnuo 2	248.39±2.15h	62.61±0.03l	-74.8	192.14^**
晚粳糯M99037 Wanjingnuo M99037	327.44±2.06c	97.55±0.03e	-70.21	250.57^**
常农粳3号Changnongjing 3	194.49±1.80k	28.50±0.02r	-85.35	208.96^**
苏香粳2号Suxiangjing 2	258.70±1.16g	34.66±0.03q	-86.6	423.73^**
苏粳8号Sujing 8	157.20±1.78n	9.53±0.02t	-93.94	186.96^**
镇稻7号Zhendao 7	400.67±2.33a	201.84±0.03a	-49.62	192.40^**
津稻1187 Jindao 1187	187.43±2.97l	64.61±0.02j	-65.53	92.43^**
津优2006 Jinyou 2006	269.46±3.27f	88.22±0.02f	-67.26	123.72^**
津原5号Jinyuan 5	277.69±5.30e	64.41±0.04k	-76.81	90.34^**
津粳杂四Jinjingzasi	218.60±4.41j	78.51±0.03h	-64.08	71.37^**
铁粳稻4号Tiejingdao 4	242.66±2.29h	50.19±0.03p	-79.32	186.06^**
辽盐稻241 Liaoyandao 241	228.63±3.49i	57.48±0.05m	-74.86	109.24^**
沈稻3号Shendao 3	215.76±3.17j	84.50±0.04g	-60.83	92.78**
长白7号Changbai 7	198.72±4.09k	50.52±0.04o	-74.56	80.10**
嘉禾218 Jiahe 218	318.70±3.80d	193.82±0.05b	-49.22	72.92**
平均值±标准差Means±s	253.22±59.85	78.75±51.22	-70.66±14.22	150.29±80.88
变化范围Range	157.20~400.67	9.53~201.84	-36.46~-93.94	71.37~423.73

供试材料 Tested materials	丙二醛含量/(μmol/g) Malondialdehyde content		变化比例/% Change ration	T-测验值 T-test value
供试材料 Tested materials	正常25 ℃ Normal temperature	低温4 ℃ Low temperature	变化比例/% Change ration	T-测验值 T-test value
浙糯36 Zhenuo 36	2.27±0.02kl	10.44±0.02d	359.05	-801.33^**
秀水52 Xiushui 52	4.68±0.03b	8.20±0.02f	75.21	-207.91^**
鄂晚稻15 Ewandao 15	3.15±0.01i	5.42±0.03j	71.97	-150.73^**
鄂晚稻13 Ewandao 13	3.96±0.01f	8.02±0.02g	102.56	-696.63^**
皖稻90 Wandao 90	4.49±0.03 c	6.22±0.02h	38.45	-99.11^**
黄糯2号Huangnuo 2	4.43±0.02d	2.11±0.03q	-52.40	165.29^**
晚粳糯M99037 Wanjingnuo M99037	5.75±0.02a	4.32±0.03n	-24.85	72.16^**
常农粳3号Changnongjing 3	3.81±0.03g	1.82±0.03r	-52.23	330.67^**
苏香粳2号Suxiangjing 2	3.94±0.02f	4.45±0.03l	12.96	-23.90^**
苏粳8号Sujing 8	3.53±0.02h	7.69±0.02b	239.62	-1 249.42^**
镇稻7号Zhendao 7	1.41±0.02m	4.64±0.02k	229.94	-809.00^**
津稻1187 Jindao 1187	1.02±0.02n	8.30±0.03e	710.51	-349.84^**
津优2006 Jinyou 2006	1.78±0.02l	2.89±0.03p	62.54	-82.72^**
津原5号Jinyuan 5	0.74±0.02o	8.22±0.02f	1 003.55	-869.77^**
津粳杂四Jinjingzasi	1.80±0.02l	10.98±0.02c	510.62	-892.03^**
铁粳稻4号Tiejingdao 4	2.33±0.02k	4.41±0.02m	89.40	-130.25^**
辽盐稻241 Liaoyandao 241	0.77±0.02o	3.57±0.02o	361.88	-1401.00^**
沈稻3号Shendao 3	4.23±0.03e	5.78±0.02i	36.74	-117.35^**
长白7号Changbai 7	2.30±0.02k	4.30±0.02n	86.68	-190.69^**
嘉禾218 Jiahe 218	2.97±0.02j	14.22±0.02a	378.05	-2 205.52^**
平均值±标准差Means±s	2.97±1.46	6.30±3.19	257.04±274.58	-485.45±609.24
变化范围Range	0.77~5.75	1.82~14.22	-52.4~1 003.55	-2 205.52~330.67

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