低温胁迫下杜鹃杂交后代优良株系的转录组分析

doi:10.7668/hbnxb.2017.05.009

摘要/Abstract

摘要： 为探索杜鹃抗寒的分子机制，筛选抗寒相关基因，利用人工模拟低温，以杜鹃杂交后代优良株系繁景-6℃（T）处理和25℃（CK）对照为试验材料，进行转录组测序和数据从头组装及生物信息学分析，通过组装得到Unigenes序列122 654个，功能注释结果显示，有大量Unigenes可以注释到基因本体（GO）和蛋白质直系同源数据库（COG）等数据库上，部分Unigenes无匹配信息，可能为杜鹃特有的基因序列。按GO功能分类，繁景杜鹃中的Unigenes分为细胞组分、分子功能以及生物学过程3类，包括51个分支，其中有大量的Unigenes与代谢进程、催化活性和细胞进程相关。上调差异表达基因有6 347个，下调差异表达基因4 482个。通过对转录结果中差异表达显著的4个转录因子家族中的24个基因，其中，上调12个，下调12个，在低温胁迫下的表达水平验证，发现其与转录结果基本一致，证明该转录组数据可靠。

关键词: 杜鹃, 低温胁迫, 转录组, 抗寒基因

Abstract: To explore the molecular mechanism of cold resistance of Rhododendron,screening of cold resistant genes,using artificial low temperature,excellent strains with Rhododendron Fanjing-6℃(T)and 25℃(CK)control as test materials,conduct transcriptome sequencing and de novo assembly data and bioinformatics analysis,the data obtained by assembling 122 654 Unigenes sequences,functional annotation showed that a large number of Unigenes could be annotated with GO and COG database,information,some of Unigenes,might be the unique gene sequence of Rhododendron. Rhododendron Fanjing transcription in the Unigenes group according to the GO function can be divided into cellular component,molecular function and biological process of 3 kinds of 51 branches,including Unigenes and metabolic process,a large number of catalytic activity and cell process. There were 6 347 up-regulated differentially expressed genes and down regulated expression genes of 4 482. The expression of 24 genes in the four transcription factors,with 12 up-regulated and 12 down-regulated at the transcription level,which were significantly different in the transcriptional results,was verified by the expression level under low temperature stress. It was found that the transcriptional data were reliable.

Key words: Rhododendron, Low temperature stress, Transcriptome, Cold-resistant genes

中图分类号:

S685.21
Q78

吴月燕, 明萌, 何静雯, 卢丹, 沈梓力, 邱甜, 吴燕燕, 谢晓鸿. 低温胁迫下杜鹃杂交后代优良株系的转录组分析[J]. 华北农学报, 2017, 32(5): 52-60. doi: 10.7668/hbnxb.2017.05.009.

WU Yueyan, MING Meng, HE Jingwen, LU Dan, SHEN Zili, QIU Tian, WU Yanyan, XIE Xiaohong. Transcriptome Analysis of Superior Strains of Rhododendron Hybrids under Low Temperature Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 52-60. doi: 10.7668/hbnxb.2017.05.009.

http://www.hbnxb.net/CN/Y2017/V32/I5/52

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