陆地棉低温响应基因<em>GhJAZ1</em>的克隆及表达分析

doi:10.7668/hbnxb.2018.01.002

华北农学报 ›› 2018, Vol. 33 ›› Issue (1): 7-13. doi: 10.7668/hbnxb.2018.01.002

所属专题： 棉花；生物技术；

陆地棉低温响应基因GhJAZ1的克隆及表达分析

蔡肖¹, 甄军波¹, 江振兴², 刘琳琳¹, 刘迪¹, 张建宏¹, 田海燕¹, 张香云¹, 迟吉娜¹

1. 河北省农林科学院棉花研究所, 农业部黄淮海半干旱区生物学与遗传育种重点实验室, 河北石家庄 050051;
2. 河北行政学院, 河北石家庄 050031

收稿日期:2017-11-19 出版日期:2018-02-28
通讯作者: 迟吉娜(1977-),女,山东掖县人,副研究员,博士,主要从事棉花功能基因组和资源创新研究。
作者简介:蔡肖(1982-),女,河北石家庄人,副研究员,博士,主要从事棉花抗逆分子生物学研究。
基金资助:
河北省自然科学基金青年项目（C2015301047）；河北省财政项目（F16R2016022579；F17R2017039379）；河北省农林科学院基本科研业务费项目（A2015070101）

Cloning and Expression Analysis of Cold Response Gene GhJAZ1 from Gossypium hirsutum L.

CAI Xiao¹, ZHEN Junbo¹, JIANG Zhenxing², LIU Linlin¹, LIU Di¹, ZHANG Jianhong¹, TIAN Haiyan¹, ZHANG Xiangyun¹, CHI Jina¹

1. Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture, Shijiazhuang 050051, China;
2. Hebei Academy of Governance, Shijiazhuang 050031, China

Received:2017-11-19 Published:2018-02-28

摘要/Abstract

摘要： 为了深入研究棉花JAZ基因在低温响应中的作用机理，利用RT-PCR方法从陆地棉中棉所36中克隆出一个低温应答基因GhJAZ1（GenBank登录号KJ562212）。全长CDS序列为810 bp，编码270个氨基酸，预测编码蛋白质的分子量为29.808 ku，理论等电点为8.33，为非跨膜蛋白。该基因编码蛋白具有一个N端NT结构域、一个TIFY结构域和一个C端Jas结构域。系统进化树分析发现，该蛋白与可可树和黄麻的亲缘关系最近。荧光实时定量PCR分析发现，GhJAZ1基因在下胚轴和根中显著高表达。此外，该基因还受到脱落酸（ABA）和茉莉酸甲酯（MeJA）的诱导表达，表达量变化趋势均为先升高后降低，但在MeJA诱导下该基因的表达量变化更明显。在低温胁迫下，不同低温耐性棉花材料中该基因的表达量变化程度也不同，耐低温品种中棉所36中GhJAZ1基因的上调表达更为显著，推测该基因参与了棉花低温胁迫防御反应。亚细胞定位显示，GhJAZ1蛋白定位于细胞核中。该研究可为进一步开展GhJAZ1基因低温响应的分子机制研究奠定基础。

关键词: 棉花, GhJAZ1, 表达分析, 低温胁迫, 亚细胞定位

Abstract: To further study the mechanism of cotton JAZ genes in low temperature response,a cold response gene GhJAZ1 was cloned from the upland cotton Zhongmiansuo 36 by RT-PCR (GenBank accession number KJ562212).The CDS sequence of GhJAZ1 was 810 bp in length and encoded 270 amino acids.The predicted molecular weight of this protein was 29.808 ku,and the theoretical isoelectric point was 8.33.It was not a transmembrane protein.The encoded protein contained a N-terminal NT domain,a TIFY domain,and a C-terminal Jas domain.Phylogenetic tree analysis showed that GhJAZ1 was much closer to that in Theobroma cacao and Corchorus olitorius.Quantitative real-time PCR analysis showed that GhJAZ1 had significantly high expression in hypocotyls and roots.In addition,the expression of GhJAZ1 was also induced by abscisic acid(ABA) and methyl jasmonate (MeJA).The trend of expression was first raised and then decreased. Under MeJA treatment, the gene expression varied more obviously. The expression pattern of GhJAZ1 varied in different cold resistant cotton materials under cold stress. The up-regulated expression of GhJAZ1 gene in cold-resistant cotton variety-Zhongmiansuo 36 was more significant, which suggesting that GhJAZ1 gene may be involved in cold response in cotton. Subcellular localization showed that GhJAZ1 protein was localized in the nucleus.This study lay foundation for further researches on the molecular mechanism of GhJAZ1 in cold response.

Key words: Gossypium, GhJAZ1, Gene expression analysis, Cold stress, Subcellular localization

中图分类号:

Q78
S567.03

蔡肖, 甄军波, 江振兴, 刘琳琳, 刘迪, 张建宏, 田海燕, 张香云, 迟吉娜. 陆地棉低温响应基因GhJAZ1的克隆及表达分析[J]. 华北农学报, 2018, 33(1): 7-13. doi: 10.7668/hbnxb.2018.01.002.

CAI Xiao, ZHEN Junbo, JIANG Zhenxing, LIU Linlin, LIU Di, ZHANG Jianhong, TIAN Haiyan, ZHANG Xiangyun, CHI Jina. Cloning and Expression Analysis of Cold Response Gene GhJAZ1 from Gossypium hirsutum L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 7-13. doi: 10.7668/hbnxb.2018.01.002.

http://www.hbnxb.net/CN/Y2018/V33/I1/7

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陆地棉低温响应基因GhJAZ1的克隆及表达分析

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陆地棉低温响应基因GhJAZ1的克隆及表达分析

Cloning and Expression Analysis of Cold Response Gene GhJAZ1 from Gossypium hirsutum L.

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