蓖麻<em>RcDELLA</em>基因的克隆及序列分析

doi:10.7668/hbnxb.2018.01.003

华北农学报 ›› 2018, Vol. 33 ›› Issue (1): 14-19. doi: 10.7668/hbnxb.2018.01.003

所属专题： 生物技术；

蓖麻RcDELLA基因的克隆及序列分析

李威¹, 李国瑞^1,2,3,4, 黄凤兰^1,2,3,4, 丛安琪¹, 李晓晨¹, 白英俊¹, 李孟建¹, 陈永胜^1,2,3,4

1. 内蒙古民族大学, 内蒙古通辽 028000;
2. 内蒙古自治区高校蓖麻产业工程技术研究中心, 内蒙古通辽 028000;
3. 内蒙古自治区蓖麻育种重点实验室, 内蒙古通辽 028000;
4. 内蒙古自治区蓖麻产业协同创新培育中心, 内蒙古通辽 028000

收稿日期:2017-10-14 出版日期:2018-02-28
通讯作者: 陈永胜(1971-),男,内蒙古通辽人,教授,博士,主要从事蓖麻分子育种研究。
作者简介:李威(1993-),女,内蒙古通辽人,在读硕士,主要从事蓖麻分子育种研究。
基金资助:
国家自然科学基金项目（31460353）；内蒙古民族大学市校合作项目（SXZD2012006；SXZD2012017）；2015年全区研究生科研创新资助项目（S20151013601）；内蒙古自治区科技创新引导项目（KJCX15002）；内蒙古自治区蓖麻产业协同创新培育中心项目；内蒙古自治区蓖麻育种重点实验室开放基金项目（MDK2016030）

Cloning and Sequence Analysis of RcDELLA Gene in Castor

LI Wei¹, LI Guorui^1,2,3,4, HUANG Fenglan^1,2,3,4, CONG Anqi¹, LI Xiaochen¹, BAI Yingjun¹, LI Mengjian¹, CHEN Yongsheng^1,2,3,4

1. Inner Mongolia University for Nationalities, Tongliao 028000, China;
2. Castor Industry Research Center of Inner Mongolia, Tongliao 028000, China;
3. Inner Mongolia Key Laboratory of Castor Breeding, Tongliao 028000, China;
4. Inner Mongolia Collaborative Innovation Cultivate Center for Castor, Tongliao 028000, China

Received:2017-10-14 Published:2018-02-28

摘要/Abstract

摘要： DELLA家族蛋白是植物GA信号途径的重要阻遏蛋白，能抑制植物的生长和发育，使植株产生矮化的表型。为了明确DELLA基因在蓖麻中的作用和功能，对RcDELLA基因进行生物学信息分析。以蓖麻2129六叶期茎尖的cDNA为模板，根据NCBI公布的DELLA （XM_002533984.2）蛋白基因片段设计引物，克隆该基因，并对其进行生物信息学分析。克隆得到该基因的完整阅读框，该基因编码序列全长为1 701 bp，编码567个氨基酸，推测其分子量为62 550.40 ku，等电点为5.14。二级结构预测表明α-螺旋占40.4%，β-折叠占8.6%，无规则卷曲占51.0%。系统进化树结果表明，RcDELLA先与大戟科巴西橡胶树和麻疯树形成分支。通过PCR扩增得到了RcDELLA基因的完整阅读框，同源性比对结果显示，RcDELLA具有典型的DELLA结构域，与NCBI上公布的其他植物的DELLA蛋白序列具有高度的同源性。系统进化树结果显示，与麻疯树亲缘关系最近，符合进化关系。生物信息学分析将为进一步研究DELLA基因在蓖麻矮化过程中的作用和功能奠定理论基础。

关键词: 蓖麻, DELLA, 序列分析, 系统进化

Abstract: DELLA family of proteins is an important repressor of plant GA signaling pathway, which can inhibit the growth and development of plants and make the plants have a dwarf phenotype. To clarify the function and function of DELLA gene in castor, conduct biological information analysis. Castor 2129 six-leaf stage shoot tip cDNA as a template,primers were designed according to the DELLA (XM_002533984.2) protein gene CDS published by NCBI, the gene was cloned and analyzed by bioinformatics. The complete reading frame of this gene was cloned,the full length of this gene was 1 701 bp, encoded 567 amino acids, molecular weight was 62 550.40 ku,isoelectric point was 5.14.The predicted secondary structure α-helix accounted for 40.4%,β-fold accounted for 8.6%,other non-random coil accounted 51.0%.Phylogenetic tree analysis showed that RcDELLA first formed a branch with Euphorbiaceae rubber tree and Jatropha curcas. The complete reading frame of RcDELLA gene was amplified by PCR, the homology comparison showed that RcDELLA had the typical DELLA domain and had high homology with the DELLA protein sequence of other plants published on NCBI. Phylogenetic tree analysis showed that the genetic relationship with Jatropha curcas was the closest and the result was consistent with species evolution.Bioinformatics analysis will lay the theoretical foundations for further study on the action and function of DELLA gene in castor dwarfing process.

Key words: Castor, DELLA, Sequence analysis, Phyletic evolution

中图分类号:

S565.03
Q78

李威, 李国瑞, 黄凤兰, 丛安琪, 李晓晨, 白英俊, 李孟建, 陈永胜. 蓖麻RcDELLA基因的克隆及序列分析[J]. 华北农学报, 2018, 33(1): 14-19. doi: 10.7668/hbnxb.2018.01.003.

LI Wei, LI Guorui, HUANG Fenglan, CONG Anqi, LI Xiaochen, BAI Yingjun, LI Mengjian, CHEN Yongsheng. Cloning and Sequence Analysis of RcDELLA Gene in Castor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 14-19. doi: 10.7668/hbnxb.2018.01.003.

http://www.hbnxb.net/CN/Y2018/V33/I1/14

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蓖麻RcDELLA基因的克隆及序列分析

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