高粱糖转运蛋白基因家族全基因组鉴定、分类及表达分析

doi:10.7668/hbnxb.201751467

摘要/Abstract

摘要： 糖转运蛋白（Sugar transporter protein，STP）基因家族在植物单糖分配中具有重要作用，并参与植物生长和发育过程中的许多代谢进程。为探究STP基因家族在高粱生长发育中的潜在功能，对高粱STP基因家族进行了全基因组鉴定、分类和组织表达分析。从高粱基因组中共鉴定出19个包含Sugar_tr结构域的STP基因，不均匀分布于9条染色体上，其中有4个基因位于基因组重复区。19个SbSTP根据其系统发育特征可以分为5组，同组的家族成员具有相同或类似的基序类型和排列顺序，但在内含子和外显子数量上存在较大差异。RNA-Seq数据分析显示，至少有18个SbSTP基因可以表达，不同的基因在不同的组织中具有不同的表达模式，其中SbSTP11特异性在花中表达，SbSTP4、SbSTP5在特定组织中受ABA和PEG的诱导表达。为进一步研究单个SbSTP基因的生物学功能，挖掘SbSTP家族的应用潜力奠定了基础。

关键词: 高粱, STP, 基因家族, 系统进化, 表达分析

Abstract: The sugar transporter(STP)gene family plays critical roles in monosaccharide distribution and participates in diverse plant metabolic processes. To investigate the potential roles of STPs in sorghum growth and development, the genome-wide identification, classification and expression analyses of the STP gene family were performed in this study. A total of 19 SbSTP genes containing the Sugar_tr conserved motifs were identified from the sorghum genome, which distributed on 9 chromosomes unevenly, and 4 of them located in the genome segmental duplication region. According to their phylogenetic features, the 19 SbSTPs were further classified into 5 main groups, with high similar motif compositions within the same group, but they were different in exon-intron number. The synteny analysis and phylogenetic comparison of STP genes from sorghum, Arabidopsis and rice provided the valuable clues for revealing the evolutionary characteristics of SbSTP genes. RNA-Seq data analysis showed that 18 of the 19 SbSTP genes could expressed in various tissues with distinct expression patterns, among which SbSTP11 exhibited tissue-specific expression in flower, while SbSTP4 and SbSTP5 expressed in root or shoot by induction of ABA and PEG stress. The results provided a valuable resource for better understanding the biological roles and application potentiality of individual STP genes in sorghum.

Key words: Sorghum bicolor, STP, Gene family, Phyletic evolution, Expression analysis

中图分类号:

Q78
S514.03

徐志军, 刘洋, 徐磊, 安东升. 高粱糖转运蛋白基因家族全基因组鉴定、分类及表达分析[J]. 华北农学报, 2019, 34(5): 65-73. doi: 10.7668/hbnxb.201751467.

XU Zhijun, LIU Yang, XU Lei, AN Dongsheng. Genome-Wide Identification, Classification and Expression Analysis of Sugar Transporter Gene Family in Sorghum bicolor[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 65-73. doi: 10.7668/hbnxb.201751467.

http://www.hbnxb.net/CN/Y2019/V34/I5/65

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