梅花花器官发育相关基因<i>PmSOC1</i>-like的功能初探

doi:10.7668/hbnxb.20190196

摘要/Abstract

摘要： SOC1/TM3（SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3）是MADS-box家族一员，它能够整合多条开花途径的开花信号，调节植物由营养生长向生殖生长的转变。在从梅花长蕊绿萼品种中克隆到3个SOC1同源基因PmSOC1-1、PmSOC1-2和PmSOC1-3的基础上，构建由CaMV35S启动子驱动的植物表达载体，并在拟南芥中过量表达，以研究其功能。通过对转基因植株表型观察发现，3个PmSOC1- like基因都具有使野生型拟南芥提前开花的作用。其中，PmSOC1-2作用最强，PmSOC1-1居中，PmSOC1-3作用最弱。对转基因拟南芥内源成花基因的qRT-PCR检测说明，PmSOC1- like基因主要是通过上调其下游花分生组织特性基因（AGL24、LFY、AP1和FUL）来促进植物开花；此外，PmSOC1-1和PmSOC1-2还导致了花瓣细丝状、花萼叶片化、花瓣和花萼宿存等花器官形态的改变，说明它们可能还具有影响花器官发育的功能。研究结果将有助于阐明梅花由营养生长向生殖生长转变的分子机制。

关键词: 梅花, PmSOC1-like, 早花, 功能分析

Abstract: The MADS-box gene SOC1/TM3 (SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1/Tomato MADS-box gene 3) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants. Three putative orthologs of SOC1 (PmSOC1-1, PmSOC1-2 and PmSOC1-3) were cloned from Prunus mume Changruilüe. The sense plant expression vectors driven by Cauliflower mosaic virus 35S promoter were constructed,and then were transformed separately into wild-type Arabidopsis plants, to study the function of PmSOC1-1, PmSOC1-2 and PmSOC1-3. Overexpression of PmSOC1-1, PmSOC1-2, and PmSOC1-3 in Arabidopsis caused early flowering. Early flowering also increased expression levels of four other flowering promoters, agamous-like 24 (AGL24), leafy (LFY), apetala 1 (AP1) and fruitfull (FUL). Moreover, the overexpression of PmSOC1-1 and PmSOC1-2 resulted in a range of floral phenotype changes such as sepals into leaf-like structures, petal color into green, petal into filament-like structures. These results suggested that the genes PmSOC1-1, PmSOC1-2 and PmSOC1-3 played an evolutionarily conserved role in promoting flowering in P. mume, and might have distinct roles during flowering development. These findings will help elucidate the molecular mechanisms involved in the transition from vegetative to reproductive development in P. mume.

Key words: Prunus mume, PmSOC1 -like, Early flowering, Functional analysis

中图分类号:

S685.17

李玉舒, 杨炜茹, 杨宇杰, 程堂仁, 王佳, 张启翔. 梅花花器官发育相关基因PmSOC1-like的功能初探[J]. 华北农学报, 2019, 34(5): 74-82. doi: 10.7668/hbnxb.20190196.

LI Yushu, YANG Weiru, YANG Yujie, CHENG Tangren, WANG Jia, ZHANG Qixiang. Functional Analysis of PmSOC1 -like Genes Related to Floral Organ Development in Prunus mume[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 74-82. doi: 10.7668/hbnxb.20190196.

http://www.hbnxb.net/CN/Y2019/V34/I5/74

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梅花花器官发育相关基因PmSOC1-like的功能初探

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