梅花<em>PmSOC1</em>-like基因的克隆与表达分析

doi:10.7668/hbnxb.2016.05.012

摘要/Abstract

摘要： SOC1/TM3（Suppressor of overexpression of constans 1/Tomato MADS-box gene 3）是MADS-box家族一员，它能够整合多条开花途径的开花信号，调节植物由营养生长向生殖生长的转变。为了解梅花成花转变过程的分子机理，采用RT-PCR的方法从梅花长蕊绿萼中克隆到3个SOC1的同源基因，分别命名为PmSOC1-1、PmSOC1-2和PmSOC1-3，并采用荧光定量PCR对3个基因的表达模式进行了分析。序列分析表明，3个基因的编码区长度分别为645 bp（PmSOC1-1）、654 bp（PmSOC1-2）和660 bp（PmSOC1-3）；编码的氨基酸长度分别为214，217，219 aa。系统进化结果显示，PmSOC1-1、PmSOC1-2和PmSOC1-3分别与拟南芥SOC1/TM3亚家族中的SOC1、AGL42/71/72和AGL14/19聚为一组。实时荧光定量分析结果表明，3个PmSOC1- like基因均在茎、叶等营养器官中表达量较高，在花、果实和种子等生殖器官中表达量较低；在梅花花芽分化过程中，3个PmSOC1- like基因的表达量整体呈下降趋势，推测PmSOC1-like基因可能在诱导梅花由营养生长向生殖生长转变过程中起重要作用。

关键词: 梅花, PmSOC1-like, 基因克隆, 表达分析

Abstract: The MADS-box gene SOC1/TM3 (Suppressor of overexpression of constans 1/Tomato MADS-box gene 3) integrates multiple flowering signals to regulate the transition from vegetative to reproductive development in plants . To better understand the molecular regulation of flower development in Prunus mume,three putative orthologs of SOC1,including PmSOC1-1, PmSOC1-2, and PmSOC1-3,were isolated.The expression patterns of PmSOC1-1, PmSOC1-2 and PmSOC1-3 were determined by Real-time RT-PCR.Each PmSOC1-like gene has a single long open reading frame(ORF) encoding a putative protein of 214,217 and 219 amino acids respectively.Phylogenetic analysis suggested that PmSOC1-1, PmSOC1-2,and PmSOC1-3 genes might be orthologous of Arabidopsis SOC1, AGL42/71/72,and AGL14/19,respectively.By Real-time quantitative RT-PCR experiment,three PmSOC1-like genes were mainly expressed in vegetative organs and expressed at low level in reproductive organs such as flowers,fruit,seed and so on.The expression of the three PmSOC1-like genes showed a decreasing trend.Above results suggest that the PmSOC1-like gene plays an important role in the transition from the vegetative growth to the reproductive growth phase.

Key words: Prunus mume, PmSOC1-like, Gene cloning, Expression analysis

中图分类号:

S685.17

李玉舒, 杨炜茹, 程堂仁, 王佳, 张启翔. 梅花PmSOC1-like基因的克隆与表达分析[J]. 华北农学报, 2016, 31(5): 78-85. doi: 10.7668/hbnxb.2016.05.012.

LI Yushu, YANG Weiru, CHENG Tangren, WANG Jia, ZHANG Qixiang. Molecular Cloning and Expression Analysis of Flowering-regulating Transcription Factor PmSOC1-like Gene in Prunus mume[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 78-85. doi: 10.7668/hbnxb.2016.05.012.

http://www.hbnxb.net/CN/Y2016/V31/I5/78

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Molecular Cloning and Expression Analysis of Flowering-regulating Transcription Factor PmSOC1-like Gene in Prunus mume

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梅花PmSOC1-like基因的克隆与表达分析

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