外源褪黑素对低温胁迫下马铃薯幼苗抗氧化系统的影响

doi:10.7668/hbnxb.20192429

摘要/Abstract

摘要：

为了探究不同浓度外源褪黑素对冷驯化后低温胁迫下马铃薯幼苗生长的影响,以云南主栽马铃薯品种合作88为材料,采用人工模拟低温胁迫(4 ℃ 14 d ,-2 ℃ 12 h)的方法,研究了不同外源褪黑素浓度(50,100,150 μmol/L)对低温胁迫下马铃薯幼苗生长及活性氧代谢系统的影响,以探讨外源MT缓解低温胁迫对马铃薯幼苗伤害的可行性。结果表明,与低温胁迫的马铃薯幼苗相比:50 μmol/L MT处理的幼苗的存活率提高了38.89百分点,株高和茎粗分别提高了2.92%,1.86%,SS、SP和Pro的含量分别增加了12.66%,13.80%,1.96%,POD和CAT的活性分别提高了29.24%,351.62%;100 μmol/L MT处理的幼苗的株高和茎粗分别提高了16.97%,2.82%,MDA和H₂O₂的含量分别降低了19.35%,0.48%,POD和CAT的活性分别提高了55.44%,213.56%,GSH的含量提高了13.61%;150 μmol/L MT处理的幼苗的存活率提高了27.78百分点,株高和茎粗分别提高了5.62%,13.20%,MDA的含量降低了9.45%,Pro的含量增加了38.70%,SOD、POD和CAT的活性分别提高了52.61%,25.25%,300.94%,AsA和GSH的含量分别提高了3.48%,3.97%。表明施加外源MT后可以不同程度的通过促进幼苗的生长、降低叶片的MDA和H₂O₂含量、促进SS、SP、Pro的积累、提高SOD、POD、CAT的活性、增加AsA和GSH的含量,从而缓解低温胁迫对马铃薯幼苗的伤害,进而提高马铃薯幼苗的抗低温胁迫的能力。综上,50 μmol/L MT处理可较优得提高马铃薯幼苗在低温胁迫下的抗逆性。

关键词: 马铃薯, 低温胁迫, 外源褪黑素, 抗氧化系统

Abstract:

In order to explore the effects of different concentrations of exogenous melatonin on the growth of potato seedlings under low temperature stress after cold acclimation,using Yunnan main potato variety Hezuo 88 as material,the effects of different concentrations of exogenous melatonin(50,100,150 μmol/L)on the growth and active oxygen metabolism system of potato seedlings under low temperature stress(4 ℃ 14 d ,-2 ℃ 12 h)were studied.The purpose of this study was to explore the feasibility of exogenous MT in alleviating the injury of potato seedlings caused by low temperature stress.The results showed that compared with potato seedlings under low temperature stress,the survival rate of seedlings treated with 50 μmol/L MT increased by 38.89 percentage point,and the plant height and stem diameter increased by 2.92% and 1.86%,the contents of SS,SP and Pro increased by 12.66%,13.80% and 1.96%,the activities of POD and CAT increased by 29.24% and 351.62%.The plant height and stem diameter of seedlings treated with 100 μmol/L MT increased by 16.97% and 2.82%,the contents of MDA and H₂O₂ decreased by 19.35% and 0.48%,the activities of POD and CAT increased by 55.44% and 213.56%.The survival rate of seedlings treated with 150 μmol/L MT increased by 27.78 percentage point,the plant height and stem diameter increased by 5.62% and 13.20%,the content of MDA decreased by 9.45%,the content of Pro increased by 38.70%,SOD,POD and CAT activity increased by 52.61%,25.25% and 300.94%,and the content of AsA and GSH increased by 3.48% and 3.97%.The results showed that exogenous MT could promote the growth of potato seedlings,reduce the content of MDA and H₂O₂ in leaves,promote the accumulation of SS,SP and Pro,increase the activities of SOD,POD and CAT,and increase the content of AsA and GSH.Thus,it can alleviate the damage of low temperature stress to potato seedlings,and then improve the ability of potato seedlings to resist low temperature stress.In summary,50 μmol/L MT treatment could better improve the stress resistance of potato seedlings under low temperature stress.

Key words: Potato, Low temperature stress, Exogenous melatonin, Antioxidant system

和秋兰, 张航, 王正维, 万丽嫱, 海梅荣. 外源褪黑素对低温胁迫下马铃薯幼苗抗氧化系统的影响[J]. 华北农学报, 2022, 37(1): 103-111. doi: 10.7668/hbnxb.20192429.

HE Qiulan, ZHANG Hang, WANG Zhengwei, WAN Liqiang, HAI Meirong. Effects of Exogenous Melatonin on Antioxidant System of Potato Seedlings under Low Temperature Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 103-111. doi: 10.7668/hbnxb.20192429.

http://www.hbnxb.net/CN/Y2022/V37/I1/103

图/表 14

图1 外源MT对低温胁迫下马铃薯幼苗表型的影响

Fig.1 Effect of exogenous melatonin on potato phenotype under low temperature stress

图2 外源褪黑素对低温胁迫下马铃薯幼苗存活率的影响
WT.常温对照;LT.低温对照;LT+50MT.低温胁迫下添加50 μmol/L褪黑素;LT+100MT.低温胁迫下添加100 μmol/L褪黑素;LT+150MT.低温胁迫下添加150 μmol/L褪黑素。不同字母表示不同处理具有显著差异(P<0.05)。图3—14同。

Fig.2 Effect of exogenous melatonin on potato survival rate under low temperature stress
WT.Normal temperature control;LT.Low temperature control;LT+50MT.Addition 50 μmol/L melatonin under low temperature stress;LT+100MT.Addition 100 μmol/L melatonin under low temperature stress;LT+150MT. Addition 150 μmol/L melatonin under low temperature stress. Different letters indicate significant difference among treatments(P<0.05).The same as Fig.3—14.

图3 外源褪黑素对低温胁迫下马铃薯幼苗株高的影响

Fig.3 Effects of exogenous melatonin on plant height of potato under low temperature stress

图4 外源褪黑素对低温胁迫下马铃薯幼苗茎粗的影响

Fig.4 Effects of exogenous melatonin on stem diameter of potato under low temperature stress

图5 外源褪黑素对低温胁迫下马铃薯幼苗叶片H₂O₂含量的影响

Fig.5 Effects of exogenous melatonin on H₂O₂ content in leaves of potato under low temperature stress

图6 外源褪黑素对低温胁迫下马铃薯幼苗叶片MDA含量的影响

Fig.6 Effects of exogenous melatonin on MDA content in leaves of potato under low temperature stress

图7 外源褪黑素对低温胁迫下马铃薯幼苗叶片可溶性糖含量的影响

Fig.7 Effects of exogenous melatonin on SS content in leaves of potato under low temperature stress

图8 外源褪黑素对低温胁迫下马铃薯幼苗叶片可溶性蛋白含量的影响

Fig.8 Effects of exogenous melatonin on SP content in leaves of potato under low temperature stress

图9 外源褪黑素对低温胁迫下马铃薯幼苗叶片脯氨酸含量的影响

Fig.9 Effects of exogenous melatonin on Pro content in leaves of potato under low temperature stress

图10 外源褪黑素对低温胁迫下马铃薯幼苗叶片SOD活性的影响

Fig.10 Effect of exogenous melatonin on SOD activity in leaves of potato under low temperature stress

图11 外源褪黑素对低温胁迫下马铃薯幼苗叶片POD活性的影响

Fig.11 Effect of exogenous melatonin on POD activity in leaves of potato under low temperature stress

图12 外源褪黑素对低温胁迫下马铃薯幼苗叶片CAT活性的影响

Fig.12 Effect of exogenous melatonin on CAT activity in leaves of potato under low temperature stress

图13 外源褪黑素对低温胁迫下马铃薯幼苗叶片AsA含量的影响

Fig.13 Effects of exogenous melatonin on AsA content in leaves of potato under low temperature stress

图14 外源褪黑素对低温胁迫下马铃薯幼苗叶片GSH含量的影响

Fig.14 Effects of exogenous melatonin on GSH content in leaves of potato under low temperature stress

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