低温胁迫对水稻苗期根系影响的蛋白质组学研究

doi:10.7668/hbnxb.20190090

摘要/Abstract

摘要： 为了从蛋白质水平揭示水稻苗期响应低温胁迫的分子机理，以耐冷性强的东乡野生稻和冷敏感品种中嘉早17为材料，于5℃低温处理0，1，2，3 d后分别测定根系活力、过氧化物酶活性、超氧化物歧化酶活性、丙二醛含量等生理指标。随后利用iTRAQ技术对5℃低温处理3 d后水稻幼苗的根系差异蛋白质组进行分析。通过生理指标分析，5℃低温处理影响东乡野生稻和中嘉早17的根系生理指标，且在低温处理第3天，两者已出现明显差异。通过差异蛋白质组学分析，与常温（25℃）相比，东乡野生稻经低温处理后共鉴定到167个差异蛋白，其中，上调表达蛋白61个，下调表达蛋白106个；中嘉早17经低温处理后共鉴定到261个差异表达蛋白，其中，上调表达蛋白122个，下调表达蛋白139个。通过GO功能分析，这些差异蛋白质主要参与代谢、细胞和单机体过程，主要涉及细胞及细胞成分，主要起着结合和催化作用。通过KEGG分析，这些差异蛋白质主要参与代谢、次级代谢产物合成和碳代谢等代谢通路。通过对东乡野生稻和中嘉早17中均发生差异表达的29个蛋白质的进一步分析，了解到两者受低温危害后根系部分蛋白质的表达动态。本研究鉴定到的差异表达蛋白质为进一步阐述水稻耐冷机理及耐冷基因的挖掘提供理论依据。

关键词: 水稻, 低温胁迫, 蛋白质组学, 苗期, 根系

Abstract: In order to reveal the molecular mechanism of response to low temperature stress at seedling stage, wild rice Dongxiang with cold tolerance and cultivar Zhongjiazao 17 with cold sensitivity were taken as the tested materials, and their root activity, peroxidase, superoxide dismutase activity and malondialdehyde content were measured under low temperature condition of 5℃ for 0, 1, 2,3 days, respectively. The differential proteomics of seedling roots in the two varieties treated at 5℃ for 3 d were analyzed by iTRAQ. The results indicated that the physiological indexes had obvious differences between the two rice materials under the treatment of 5℃ for 3 d. The differential proteomics analysis showed that a total of 167 differentially expressed proteins were identified in Dongxiang wild rice, of which 61 were up-regulated and 106 down-regulated, and a total of 261 differentially expressed proteins were identified in cultivar Zhongjiazao 17, of which 122 were up-regulated and 139 down-regulated. GO analysis indicated that these differentially expressed proteins mainly participated in metabolic process, celluar process, and single-organism process related to cell and cell part, playing the binding and catalytic effects. KEGG analysis showed that these differentially expressed proteins mainly related to metabolic pathways, biosynthesis of secondary metabolites and carbon metabolism. Through further analysis of 29 proteins expressed differentially in both Dongxiang wild rice and cultivar Zhongjiazao 17, the expression dynamics of some proteins in root was obtained after they were damaged by low temperature. The identification of differentially expressed proteins would be helpful to discover the cold tolerance genes and explain the cold tolerance mechanism of rice.

Key words: Rice, Low temperature stress, Proteomics, Seedling stage, Root

中图分类号:

S332.5

唐秀英, 王会民, 龙起樟, 黄永兰, 芦明, 万建林. 低温胁迫对水稻苗期根系影响的蛋白质组学研究[J]. 华北农学报, 2019, 34(6): 82-88. doi: 10.7668/hbnxb.20190090.

TANG Xiuying, WANG Huimin, LONG Qizhang, HUANG Yonglan, LU Ming, WAN Jianlin. Proteomics Analysis of Rice Root under Low Temperature Stress at Seedling Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 82-88. doi: 10.7668/hbnxb.20190090.

http://www.hbnxb.net/CN/Y2019/V34/I6/82

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