花生蛋白磷酸酶2C家族基因的鉴定和盐胁迫响应分析

doi:10.7668/hbnxb.2018.03.012

华北农学报 ›› 2018, Vol. 33 ›› Issue (3): 71-77. doi: 10.7668/hbnxb.2018.03.012

所属专题： 油料作物；盐碱胁迫；生物技术；

花生蛋白磷酸酶2C家族基因的鉴定和盐胁迫响应分析

陈冠旭¹, 秦贵龙¹, 李恩广¹, 赵春梅¹, 乔利仙^1,2, 王晶珊^1,2, 隋炯明^1,2

1. 青岛农业大学生命科学学院, 山东青岛 266109;
2. 青岛农业大学农学院, 山东省旱作技术重点实验室, 山东青岛 266109

收稿日期:2017-10-10 出版日期:2018-06-28
通讯作者: 隋炯明(1978-),男,山东诸城人,副教授,博士,主要从事作物分子育种研究。
作者简介:陈冠旭(1992-),男,安徽界首人,在读硕士,主要从事植物基因工程研究。
基金资助:
国家自然科学基金项目（31571705；31301356；31471542）；山东省科技发展计划项目（2014GNC110002）

Identification and Salinity Stress-responsive Analysis of PP2C Genes in Peanut

CHEN Guanxu¹, QIN Guilong¹, LI Enguang¹, ZHAO Chunmei¹, QIAO Lixian^1,2, WANG Jingshan^1,2, SUI Jiongming^1,2

1. College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, China;
2. College of Agriculture, Qingdao Agricultural University, Dry-land Farming Technology Laboratory, Qingdao 266109, China

Received:2017-10-10 Published:2018-06-28

摘要/Abstract

摘要： 为了分析花生中蛋白磷酸酶2C基因（PP2C）的情况，对耐盐花生突变体（S2）和对照（S4）在胁迫处理前后的叶片进行RNA-Seq分析。通过生物信息学分析筛选出了79个具有完整开放阅读框的PP2C基因，它们位于花生A组野生种的10条染色体上，不同染色体上的PP2C基因数目差异很大，分布为1~15个。根据拟南芥中的PP2C基因的聚类分析结果，79个花生PP2C基因能聚到已报道的12个亚族15个亚类。为了分析这些PP2C基因对盐胁迫响应情况，利用突变体S2和对照S4构建了盐胁迫处理前后的表达谱，筛选出35个受盐胁迫诱导表达的PP2C基因，涉及12个亚族14个亚类，其中A亚族b亚类、G亚族b亚类、I亚族、L亚族所有成员均受盐胁迫诱导表达；而G亚族a亚类的所有成员均不受盐胁迫诱导表达。对这35个PP2C基因的启动子研究发现，绝大多数PP2C基因的启动子存在多种胁迫响应元件：如与高温胁迫相关的调控元件HSE、与干旱诱导相关的MYB转录因子结合位点MBS、逆境胁迫应答元件TC-rich repeats、与抗氧化反应相关的ARE元件等。为花生PP2C基因的功能研究和利用奠定了基础。

关键词: 花生, PP2C基因, 盐胁迫, 顺式调控元件

Abstract: In order to elucidate the role of protein phosphatase 2C genes (PP2C) in peanut,RNA sequencing (RNA-Seq) was conducted with leaves of a salinity tolerant peanut mutant (S2) and its control (S4) before and after salinity treatment as materials in this experiment. 79 PP2C genes with complete open reading frame were screened to distribute on 10 chromosomes of A genome,the gene number of each chromosome was significantly different with the variation range of 1 to 15. According to the clustering result of PP2C genes from Arabidopsis,these 79 PP2C genes from peanut were divided into 12 subgroups and 15 subkinds. To analyze the response to salinity stress of these 79 PP2C genes,digital gene expression profiles were constructed with S2 and S4 before and after salinity treatment. The results showed that 35 PP2C genes belonging to 12 subgroups and 14 subclasses were responsive to salinity stress including all the members belonging to b subclass of A subgroup,b subclass of G subgroup,I subgroup and L subgroup;while all the members belonging to a subclass of G subgroup were insensitive to salinity stress. Some important stress-responsive elements were found in promoters of the most of these 35 PP2C genes,such as heat shock element (HSE),MYB binding site involved in drought inducibility (MBS),stress responsive element TC-rich repeats and antioxidant-response element (ARE),etc. The results could provide basis for functional research and the application of PP2C genes in peanut.

Key words: Peanut, PP2C gene, Salinity stress, cis-regulatory element

中图分类号:

Q785

陈冠旭, 秦贵龙, 李恩广, 赵春梅, 乔利仙, 王晶珊, 隋炯明. 花生蛋白磷酸酶2C家族基因的鉴定和盐胁迫响应分析[J]. 华北农学报, 2018, 33(3): 71-77. doi: 10.7668/hbnxb.2018.03.012.

CHEN Guanxu, QIN Guilong, LI Enguang, ZHAO Chunmei, QIAO Lixian, WANG Jingshan, SUI Jiongming. Identification and Salinity Stress-responsive Analysis of PP2C Genes in Peanut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(3): 71-77. doi: 10.7668/hbnxb.2018.03.012.

http://www.hbnxb.net/CN/Y2018/V33/I3/71

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