华北农学报 ›› 2018, Vol. 33 ›› Issue (1): 60-64. doi: 10.7668/hbnxb.2018.01.010

所属专题: 玉米 生物技术

• 论文 • 上一篇    下一篇

玉米叶夹角的全基因组关联分析

孙娇1,3, 赵美爱1,3, 潘顺祥1,3, 裴玉贺2,3, 郭新梅2,3, 宋希云2,3   

  1. 1. 青岛农业大学 生命科学学院, 山东 青岛 266109;
    2. 青岛农业大学 农学与植物保护学院, 山东 青岛 266109;
    3. 青岛农业大学 青岛市主要农作物种质创新与应用重点实验室, 山东 青岛 266109
  • 收稿日期:2017-12-09 出版日期:2018-02-28
  • 通讯作者: 宋希云(1961-),男,山东潍坊人,教授,博士,主要从事玉米遗传育种、生化与分子生物学研究。
  • 作者简介:孙娇(1991-),女,山东烟台人,在读硕士,主要从事作物遗传育种研究。
  • 基金资助:
    国家自然科学基金项目(31371636);山东省现代农业产业技术体系玉米产业创新团队项目(SDAIT-01-022-01);山东省农业良种工程;山东省农业生物资源创新利用研究课题;青岛市应用基础研究计划项目(14-2-4-13-jch)

Correlation Analysis of Maize Leaf Angle with Genome-wide Association Analysis

SUN Jiao1,3, ZHAO Meiai1,3, PAN Shunxiang1,3, PEI Yuhe2,3, GUO Xinmei2,3, SONG Xiyun2,3   

  1. 1. College of Life Science, Qingdao Agricultural University, Qingdao 266109, China;
    2. College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China;
    3. Qingdao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2017-12-09 Published:2018-02-28

摘要: 为了阐述玉米叶夹角的遗传基础并定位相关的数量性状位点,利用289份常用玉米自交系为试验材料,在自然条件下测量玉米穗上叶夹角,对其进行性状分析,结果表明,各个环境的穗上叶夹角数据呈正态分布,并且各个环境间的数据呈极显著正相关。同时采用MaizeSNP50基因芯片进行基因分型,利用R平台下的farmCPU对叶夹角进行全基因组关联分析。在4个环境中共检测到42个与玉米叶夹角显著关联(P < 0.000 01)的SNP,其中1,5,8号染色体在4个环境下都检测到与穗上叶夹角紧密关联的SNP标记。对筛选出的所有标记综合分析,共鉴定出15个与玉米叶夹角相关的SNP标记位点,分布在Bin1.02、Bin1.03、Bin1.06、Bin1.11、Bin2.05、Bin3.04、Bin5.03、Bin5.04、Bin8.03、Bin8.04、Bin10.07处。采用全基因组关联分析的方法发掘叶夹角基因位点及候选基因,对揭示玉米叶夹角的遗传机理,加速玉米育种进程具有重要的意义。

关键词: 叶夹角, 相关性分析, 关联分析

Abstract: To illustrate the genetic basis of maize leaf angle and locate the relevant quantitative trait loci,using 289 maize inbred lines was commonly used as test materials,under natural conditions on the measurement of corn leaf angle,analyzing its traits,the results showed that each environment on the ear of leaf angle data normally distributed,and the data had very significantly positive correlation between the various environments.At the same time,the genotyping of the MaizeSNP50 gene chip was carried out,and the whole genome correlation analysis was carried out using farmCPU.Detected in four environments the communist party of China and corn leaf angle significant correlation (P <0.000 01) of SNP,chromosome 1,5,8 of them in four conditions were detected and ear leaf angle was closely related to SNP marker.Comprehensive analysis to filter out all the tags that were identified in 15 SNP marker loci associated with maize leaf angle distribution in Bin1.02,Bin1.03,Bin1.06,Bin1.11,Bin2.05,Bin3.04,Bin5.03,Bin5.04,Bin8.03,Bin8.04,Bin10.07 place.It is important to explore the genetic mechanism of maize leaf angle and accelerate maize breeding process by using the method of genome-wide association analysis to discover the gene locus of leaf and the candidate genes.

Key words: Length angle, Correlation analysis, Association analysis

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引用本文

孙娇, 赵美爱, 潘顺祥, 裴玉贺, 郭新梅, 宋希云. 玉米叶夹角的全基因组关联分析[J]. 华北农学报, 2018, 33(1): 60-64. doi: 10.7668/hbnxb.2018.01.010.

SUN Jiao, ZHAO Meiai, PAN Shunxiang, PEI Yuhe, GUO Xinmei, SONG Xiyun. Correlation Analysis of Maize Leaf Angle with Genome-wide Association Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(1): 60-64. doi: 10.7668/hbnxb.2018.01.010.

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