玉米<em>ZmMGT0</em>基因过表达载体的构建及拟南芥遗传转化研究

doi:10.7668/hbnxb.2017.06.001

华北农学报 ›› 2017, Vol. 32 ›› Issue (6): 1-7. doi: 10.7668/hbnxb.2017.06.001

所属专题： 玉米；生物技术；

玉米ZmMGT0基因过表达载体的构建及拟南芥遗传转化研究

李洪有^1,2, 张素芝², 陈庆富¹

1. 贵州师范大学荞麦产业技术研究中心, 贵州贵阳 550001;
2. 四川农业大学玉米所, 农业部西南玉米生物学与遗传育种重点实验室, 四川成都 611130

收稿日期:2017-09-15 出版日期:2017-12-28
通讯作者: 陈庆富(1966-),男,贵州铜仁人,教授,博士,主要从事植物遗传育种研究。
作者简介:李洪有(1987-),男,四川资阳人,助理研究员,博士,主要从事植物分子生物技术研究。
基金资助:
贵州省高层次创新型人才培养计划项目（20154020）

Construction of Overexpression Vector of Maize ZmMGT10 Gene and Transformation of Arabidopsis thaliana

LI Hongyou^1,2, ZHANG Suzhi², CHEN Qingfu¹

1. Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China;
2. Maize Research Institute, Sichuan Agricultural University, Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu 611130, China

Received:2017-09-15 Published:2017-12-28

摘要/Abstract

摘要： CorA/MRS2/MGT-型镁离子转运蛋白在维持植物镁离子平衡中具有重要作用。为探讨一个表达受缺镁胁迫诱导的玉米MRS2/MGT-型镁离子转运蛋白基因ZmMGT10在缺镁胁迫中的功能，构建了ZmMGT10的过表达载体并遗传转化拟南芥，获得了转基因拟南芥株系。同野生型拟南芥植株相比，过表达ZmMGT10增加了低镁胁迫生长下转基因植株的生物量、根长和叶绿素浓度。进一步研究表明，在低镁生长条件下，转基因植株的根和叶中积累的镁离子含量均明显高于野生型植株。此外，在低镁生长条件下，转基因植株根对镁离子的吸收能力明显强于野生型植株。结果表明，过表达ZmMGT10基因可以增强转基因拟南芥植株对低镁胁迫的抵抗。

关键词: 玉米, ZmMGT10, 镁离子转运蛋白, 拟南芥, 转基因植株

Abstract: CorA/MRS2/MGT-type magnesium ion transporters play an important role in maintaining magnesium homeostasis in plants.In order to investigate the function of ZmMGT10 gene which is a maize CorA/MRS2/MGT-type magnesium ion transporter and expression is induced by magnesium deficiency,we constructed the overexpression vector of ZmMGT10 and transgenic Arabidopsis plants were obtained.Compared with wild-type plants,transgenic Arabidopsis plants grew more vigorously than wild-type plants under low Mg conditions,exhibited by longer root length,higher plant fresh weight and chlorophyll content.Further analysis indicated that the roots and shoots of transgenic plants had higher magnesium accumulation than wild-type plants under low magnesium condition.Furthermore,the roots of transgenic plants had enhanced magnesium uptake ability than wild-type plants.The results suggested that overexpression ZmMGT10 in Arabidopsis could enhance the resistance ability of transgenic plants to magnesium deficiency stress.

Key words: Maize, ZmMGT10, Magnesium ion transporter, Arabidopsis, Transgenic plants

中图分类号:

Q78
S513.03

李洪有, 张素芝, 陈庆富. 玉米ZmMGT0基因过表达载体的构建及拟南芥遗传转化研究[J]. 华北农学报, 2017, 32(6): 1-7. doi: 10.7668/hbnxb.2017.06.001.

LI Hongyou, ZHANG Suzhi, CHEN Qingfu. Construction of Overexpression Vector of Maize ZmMGT10 Gene and Transformation of Arabidopsis thaliana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(6): 1-7. doi: 10.7668/hbnxb.2017.06.001.

http://www.hbnxb.net/CN/Y2017/V32/I6/1

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玉米ZmMGT0基因过表达载体的构建及拟南芥遗传转化研究

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玉米ZmMGT0基因过表达载体的构建及拟南芥遗传转化研究

Construction of Overexpression Vector of Maize ZmMGT10 Gene and Transformation of Arabidopsis thaliana

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