抗条锈病基因<i>YrCH5026</i>的遗传分析及分子定位

doi:10.7668/hbnxb.2015.05.002

摘要/Abstract

摘要： CH5026是携带中间偃麦草抗病基因的渗入系。为了更好地利用CH5026,拓宽小麦抗性育种资源,对其抗条锈性来源和遗传模式进行了分析,对抗性基因进行了染色体定位并构建了遗传连锁图谱。在苗期和成株期对CH5026及其亲本分别接种条锈菌流行小种CYR31、CYR32和CYR33。结果表明,CH5026在苗期和成株期对这3个条锈菌小种均表现出免疫或近免疫,且与其抗性供体TAI7045及其野生亲本中间偃麦草抗病侵染型相似。对其与感病品种(系)的杂交后代F₁、F₂、F_2:3和BC₁群体接种CYR32进行成株期抗性遗传机制分析,证实CH5026对CYR32的抗性由1对显性核基因控制。基因组原位杂交未检测到外源DNA杂交信号。用569对SSR引物对CH5026/台长29的192个F₂群体进行分析,发现3个与抗性基因连锁的SSR标记: Xgwm210、Xwmc382 和 Xgpw7101,抗性基因位点与两翼邻近连锁标记 Xwmc382 和 Xgpw7101 的遗传距离分别为6.0,4.7 cM。利用中国春缺四体、双端体材料将该基因及其连锁标记定位在染色体2AS上。通过基因来源及连锁分子标记多态性比较,这个抗条锈病基因与已知定位于染色体2AS上的抗性基因不同,很可能是一个新的抗条锈病新基因,暂将其命名为 YrCH5026。

关键词: 小麦, 中间偃麦草, 渗入系, 条锈病, 分子定位

Abstract: CH5026 is a stable introgression line carrying resistance genes of Thinopyrum intermedium.To better utilize CH5026,increase the genetic resources,this study were to characterize the origin of the resistance,determine the inheritance of resistance,locate the chromosome and construct the linkage map.Using three prevalent races of stripe rust CYR31,CYR32 and CYR33, Th.intermedium (accession Z1141),CH5026 and its resistant parent amphiploid TAI7045 as well as all wheat parents were evaluated by inoculated races in the seedling stage and adult stages.The result showed that CH5026 and its donor TAI7045 as well as the wild parent were resistant at the seedling and adult stages to CYR31,CYR32 and CYR33,whereas all the wheat parents involved were susceptible,indicating that the CH5026 was possibly derived from Th.intermedium. No Th.intermedium chromatin has been detected according to genome in situ hybridization assay.Genetic analysis on the F₁,F₂,F_2:3 and BC₁ populations from stripe rust resistant line CH5026 revealed that a single dominant gene resistance to CYR32.A total of 192 F₂ populations from the cross CH5026/Taichang 29(TC 29)was screened with 569 wheat SSR primer pairs to detect molecular markers linked to resistance gene.Three SSR primers, Xgwm210, Xwmc382 and Xgpw7101 were identified to be linked to the resistance gene.The two flanking markers were Xwmc382 and Xgpw7101,genetic distances were 6.0,4.7 cM,respectively.Using the Chinese Spring(CS)nulli-tetrasomic and ditelosomic lines,the resistance gene and its linked markers were assigned to chromosome arm 2AS.Based on the source of the Yr genes and ploymorphism of linked molecular markers,the stripe rust resistance gene in CH5026 was likely different from the known resistance gene on chromosome 2AS.The gene might be a novel stripe rust resistance gene,this new resistance gene is temporarily named YrCH5026.

Key words: Wheat, Thinopyrum intermedium, Introgression line, Stripe rust, Molecular mapping

中图分类号:

S435.1
Q78

侯丽媛, 乔麟轶, 张晓军, 李欣, 詹海仙, 畅志坚. 抗条锈病基因YrCH5026的遗传分析及分子定位[J]. 华北农学报, 2015, 30(5): 7-15. doi: 10.7668/hbnxb.2015.05.002.

HOU Li-yuan, QIAO Lin-yi, ZHANG Xiao-jun, LI Xin, ZHAN Hai-xian, CHANG Zhi-jian. Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrCH5026[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(5): 7-15. doi: 10.7668/hbnxb.2015.05.002.

http://www.hbnxb.net/CN/Y2015/V30/I5/7

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抗条锈病基因YrCH5026的遗传分析及分子定位

Genetic Analysis and Molecular Mapping of a Stripe Rust Resistance Gene YrCH5026

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