摘要： 为了研究 HKT1 在普通小麦及其野生近缘种中的自然多样性,揭示 HKT1 结构与功能的关系,采用克隆测序的方法对38份普通小麦和13份小麦野生近缘种 HKT1 基因组序列进行了分析。在38份普通小麦材料中共发现5种 HKT1 序列,分别命名为 HKT1-1～HKT1-5,其中29份材料仅含有1种类型 HKT1。 而在13份小麦野生近缘种中发现19种 HKT1,其中根据 HKT1 基因组序列预测13种有完整读码框。普通六倍体小麦的核苷酸多样性仅相当于其野生近缘种的23.8%。这些结果表明, HKT1 在小麦基因组中属于多拷贝基因, HKT1 在栽培驯化过程中受到了选择,属于驯化基因。利用中国春小麦缺四体和W7984×Opata85作图群体,将 HKT1 基因定位于7B染色体长臂。

关键词: 小麦, HKT1, 基因多样性, 基因驯化

Abstract: In order to analyze natural diversity of HKT1 in common wheat and wheat wild relatives and the relationship of structure and function of wheat HKT1, HKT1 genomic sequences of 38 common wheat varieties and 13 wheat wild relatives were surveyed with PCR cloning and sequencing.Five sequence types existed in the 38 common wheat varieties,named as HKT1-1-HKT1-5 respectively,and 29 varieties only contained one type.Nineteen types were found in 13 wheat wild relatives,among which 13 types were predicted to have complete coding sequencings.Natural diversity of wheat wild relatives was more abundant than that of the common wheat.The diversity of HKT1 in common wheat equaled to 23.8% of that in wild relatives.The results showed that HKT1 was selected during wheat domestication and belonged to a multicopy gene.By using Chinese Spring nullisomic-tetrasomic lines and W7984× Opata85 mapping population, HKT1 was mapped on the long arm of chromosome 7B.

Key words: Wheat, HKT1, Gene diversity, Gene domestication

中图分类号: 

• S512.03

• Q78