华北农学报 ›› 2014, Vol. 29 ›› Issue (S1): 29-34. doi: 10.7668/hbnxb.2014.S1.007

所属专题: 水产

• 论文 • 上一篇    下一篇

瓦氏雅罗鱼群体基于CO Ⅰ序列的遗传多样性分析

窦新杰1,2, 常玉梅1, 唐然1, 孙效文1, 陶然1,3, 王楠1,2, 梁利群1, 张丽霞4   

  1. 1. 中国水产科学研究院 黑龙江水产研究所, 淡水鱼类育种国家地方联合工程实验室, 农业部淡水水产生物技术与 遗传育种重点实验室, 黑龙江 哈尔滨 150070;
    2. 上海海洋大学 水产与生命学院, 上海 201306;
    3. 宁波大学 海洋学院, 浙江 宁波 315211;
    4. 内蒙古达拉特旗水产工作站, 内蒙古 鄂尔多斯 014300
  • 收稿日期:2014-10-10 出版日期:2014-12-28
  • 通讯作者: 梁利群(1963-),女,黑龙江望奎人,研究员,主要从事鱼类遗传育种研究。
  • 作者简介:窦新杰(1988-),男,山东潍坊人,在读硕士,主要从事鱼类遗传育种研究。
  • 基金资助:
    国家高技术研究发展计划项目(2011AA100402-3);黑龙江省科技攻关项目(GC12B307)

Genetic Diversity of Leuciscus waleckii Populations Based on CO ⅠSequence

DOU Xin-jie1,2, CHANG Yu-mei1, TANG Ran1, SUN Xiao-wen1, TAO Ran1,3, WANG Nan1,2, LIANG Li-qun1, ZHANG Li-xia4   

  1. 1. Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences, National & Local United Engineering Laboratory of Freshwater Fish Breeding, Key Laboratory of Freshwater Aquatic Biotechnology and Genetic Breeding, Ministry of Agriculture, Harbin 150070, China;
    2. College of Fisheries and Life Scince, Shanghai Ocean University, Shanghai 201306, China;
    3. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    4. Inner Mongolia Dalate's Aquatic Workstations, Ordos 014300, China
  • Received:2014-10-10 Published:2014-12-28

摘要: 利用mtDNA CO Ⅰ基因片段(有效长度635 bp)对来自达里湖 (DL)、岗更湖(GG)、呼伦湖(HL)、黑龙江(HLJ)、松花江(SHJ)和乌苏里江(WSL) 6个地理群体共72个样品进行了遗传多样性和遗传结构分析。从碱基组成来看,6个地理群体在CO Ⅰ区段上的A+T含量(52.37%)明显高于G+C含量(47.67%),符合脊椎动物的碱基组成特点;从各项遗传参数来看,达里湖流域2个群体(DL和GG)的遗传多样性低于黑龙江流域4个群体(HL、HLJ、SHJ、WSL);6个群体共检测到20个单倍型,并共享1个单倍型,达里湖流域群体共享1个单倍型,黑龙江流域群体共享1个单倍型。虽然达里湖流域和黑龙江流域各群体间遗传分化明显(P<0.05),遗传结构存在差异,但AMOVA检验不支持这种遗传差异;另外,群体间频繁的基因交流进一步证实,6个地理群体亲缘关系近,有共同的起源,尚未达到分化为不同的种或亚种的水平。

关键词: 瓦氏雅罗鱼, CO Ⅰ基因, 遗传多样性, 遗传分化

Abstract: To analyzing the genetic diversity and genetic structure of Leuciscus waleckii based on mtDNA CO Ⅰgene(635 bp length),six geographical populations(72 samples) were sampled from Dali Lake(DL),Ganggeng Lake(GG),Hulun Lake(HL),Amur River(HLJ),Songhua River(SHJ) and Wusuli River(WSL).On the base composition,A+T content(52.37%) was slightly higher than that of G+C content(47.63%),corresponding to the characteristics of nucleotide composition in the vertebrates;On the genetic parameters,the genetic diversities of Dali Basin populations(DL and GG) were lower than those of Amur Basin counterparts(HL,HLJ,SHJ and WSL);There were 20 haplotypes,of one haplotype was shared by all the six populations,one shared by Dali Basin and one shared by Amur Basin.Despite there were significant population differentiation between Dali Basin and Amur Basin(P<0.05) as well as genetic structure,the analysis of AMOVA and the values of frequent gene exchange(Nm) both demonstrated that the six Leuciscus waleckii populations had very close relationship,probably derived from a common ancestor,and had not yet differentiated to different species or subspecies based on present evidence.

Key words: Leuciscus waleckii, CO Ⅰgene, Genetic diversity, Genetic differentiation

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引用本文

窦新杰, 常玉梅, 唐然, 孙效文, 陶然, 王楠, 梁利群, 张丽霞. 瓦氏雅罗鱼群体基于CO Ⅰ序列的遗传多样性分析[J]. 华北农学报, 2014, 29(S1): 29-34. doi: 10.7668/hbnxb.2014.S1.007.

DOU Xin-jie, CHANG Yu-mei, TANG Ran, SUN Xiao-wen, TAO Ran, WANG Nan, LIANG Li-qun, ZHANG Li-xia. Genetic Diversity of Leuciscus waleckii Populations Based on CO ⅠSequence[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 29-34. doi: 10.7668/hbnxb.2014.S1.007.

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