华北农学报 ›› 2013, Vol. 28 ›› Issue (3): 145-151. doi: 10.3969/j.issn.1000-7091.2013.03.026

所属专题: 油料作物 生物技术

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大豆酸性磷酸酶活性变化及磷高效基因型的筛选与相关分析

刘渊, 李喜焕, 张彩英   

  1. 河北农业大学农学院华北作物种质资源研究与利用重点实验室, 河北保定 071001
  • 收稿日期:2013-03-21 出版日期:2013-06-28
  • 作者简介:刘渊(1978-),女,河北邯郸人,助理研究员,在读博士,主要从事作物育种目标性状遗传与改良研究
  • 基金资助:
    国家转基因重大专项(2009ZX08004-004B);国家自然科学基金项目(31071441);河北省自然科学基金项目(C2010000749)

Relativity Analysis between Acid Phosphatase Activity and Screening Genotypic Variation in P Efficiency of Soybean

Liu Yuan, Li Xihuan, Zhang Caiying   

  1. North China Key Laboratory of Crop Germplasm Resources,College of Agronomy, Hebei Agricultural University, Baoding 071001,China
  • Received:2013-03-21 Published:2013-06-28

摘要: 分析不同磷处理下的大豆幼叶、根尖酸性磷酸酶活性与植株磷利用率间的相关性,提供能够快速准确筛选磷高效利用大豆基因型的生化指标;在3种不同磷处理水平下,测定并比较12个大豆品种的幼叶、根尖酸性磷酸酶活性以及植株磷利用率;检测2μmol/L磷处理下的159份大豆品种的根尖酸性磷酸酶活性。2μmol/L磷处理下,根尖酸性磷酸酶活性与磷利用率间存在极显著正相关(r>r0.05),并从供试171份材料中筛选出6个磷高效大豆基因型;低磷条件下,大豆根尖酸性磷酸酶活性可作为磷高效基因型筛选的重要生化指标。

关键词: 大豆, 酸性磷酸酶活性, 磷利用率, 磷高效品种

Abstract: Study the relationships between the shoot or root acid phosphatase activity ( APA) and phosphorus utilization ratio under different phosphorus treatments, to provide a basis for screening P efficiency varieties in soybean.Under three different P supply levels, the soybean shoot and root acid phosphatase activities and P utilization ratio of 12 genotypes were determinated and contrasted. The root acid phosphatase activities of 159 genotypes were also detected under 2 μmol /L P concentration. The results showed that soybean root acid phosphatase activity was positively correlated with P utilization ratio significantly ( r > r0. 01) under 2 μmol /L P concentration. Six phosphorus efficiency varieties were screened out from 171 experimental genotypes. Under low phosphorus concentration, soybean root acid phosphatase activity coule be used as an important biochemistry index to screen P efficient genotype.

Key words: Soybean, Acid phosphatase activity( APA) , P utilization ratio, P efficient varieties

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引用本文

刘渊, 李喜焕, 张彩英. 大豆酸性磷酸酶活性变化及磷高效基因型的筛选与相关分析[J]. 华北农学报, 2013, 28(3): 145-151. doi: 10.3969/j.issn.1000-7091.2013.03.026.

Liu Yuan, Li Xihuan, Zhang Caiying. Relativity Analysis between Acid Phosphatase Activity and Screening Genotypic Variation in P Efficiency of Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 145-151. doi: 10.3969/j.issn.1000-7091.2013.03.026.