梯度减库对粳稻内源激素含量及粒质量形成的影响

doi:10.3969/j.issn.1000-7091.2013.03.025

摘要/Abstract

摘要： 为明确籽粒内激素含量变化动态与源库矛盾的关系,通过梯度减除稻穗上部枝梗的方法,改变不同穗位籽粒的源库关系,进而研究了不同粒位籽粒的IAA、ABA、ZT、GA3的动态变化及其与胚乳细胞增殖、粒质量形成之间的关系。结果表明,籽粒中激素以IAA、ABA含量较大,而ZT、GA3含量较小;自然状态下,粒位间IAA含量总的变化趋势是灌浆前期下位籽粒大于上位籽粒,灌浆后期则相反;其他激素总体表现为灌浆前期上位籽粒大于下位籽粒,灌浆后期则相反;粒位间激素含量差异存在波动。源库比例改变后,各粒位激素含量总体提高、弱势粒的变化幅度较大,粒位间、激素间存在不同的变化特征;相同粒位激素含量变化幅度并不随减库程度的增加而增加。灌浆中期的激素含量对胚乳细胞增殖有延后效应,灌浆前期及后期的激素含量对籽粒干质量增加既存在即时效应,又存在延后效应。

关键词: 粳稻, 内源激素, 源库关系, 粒位, 粒质量

Abstract: In this study，a gradient reduction sink method was used to control the source /sink ratio to illustrate the relationship between phytohomone levels and source /sink ratio in rice grains． We investigated IAA，ABA，ZT and GA3 levels for the grains from different position of the panicle and studied the relationship of phytohormone dynamic changes with the endosperm cell increasing rates as well as with the grain development． Our results showed that the contents of ABA and IAA were higher，whilst the contents of ZT and GA3 were lower in grains． Before grain filling stage， the IAA content of lower grains was higher than that of upper grains，whilst it was contrary after grain filling stage． For the other phytohormones， the contents of upper grains were higher than that of lower grains，and it was also contrary after grain filling stage． There was fluctuation in the differences among phytohormones of different position grains． After the source /sink ratio was changed， the overall content of phytohormone increased，and the weak sink grains showed a wider changing rang． Different position grains and different phytohormones showed different changing characters． The changing range of phytohormone for the different treat and same position grains did not add to the degree of grain treatment． The phytohormone levels showed delayed effects for the endosperm cell increasing during grain filling stage． Whilst the phytohormone levels showed both delay and instant effects for grain weight increase before and after grain filling stage

Key words: Japonica rice, Endogenous hormone, Source-sink relationship, Grain position, Grain weight

中图分类号:

S511.01

刘颖, 呼楠, 程海涛, 吕文彦, 曹萍, 郎慧敏, 刘桂林. 梯度减库对粳稻内源激素含量及粒质量形成的影响[J]. 华北农学报, 2013, 28(3): 139-144. doi: 10.3969/j.issn.1000-7091.2013.03.025.

Liu Ying, Hu Nan, Cheng Haitao, Lü Wenyan, Cao Ping, Lang Huimin, Liu Guilin. Effects of Gradient Reduction Sink on Endogenous Phytohormone Levels and Grain Development in japonica Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 139-144. doi: 10.3969/j.issn.1000-7091.2013.03.025.

http://www.hbnxb.net/CN/Y2013/V28/I3/139

参考文献

[1] Myers P N ，Setter T L，Madison J T， et al． Abscisic aqcid inhibition of endosperm cell division in cultured Maize Kernels[J]． Plant Physiol， 1990， 94， 1330 - 1336．
[2] Yang J，Zhang J，Wang Z， et al． Hormonal changes in the grains of rice subjected to water stress during grain filling[J]． Plant Physiol， 2001， 127: 315 - 323．
[3] Schruff M C，Spielman M，Tiwari S， et al． The Auxin Response Factor 2 gene of Arabidopsis links auxin signalling，cell division， and the size of seeds and other organs[J]． Development， 2006 ， 133， 251 - 261．
[4] Day R C，Herridge R P，Ambrose B A， et al． Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division，cytokinin signaling， and gene expression regulation[J]． Plant Physiol， 2008， 148(4) : 1964 - 1984．
[5] 陶龙兴，王熹，黄效林．内源IAA 对杂交稻强、弱势粒灌浆增重的影响[J]．中国水稻科学，2003，17(2) :149 - 155．
[6] 张祖建，朱庆森，王志琴，等．水稻品种源库特性与胚乳细胞增殖和充实的关系[J]．作物学报，1998，24(1) : 21 - 26．
[7] 唐塘，吕冰，梁建生．不同水稻品种( 组合) 籽粒灌浆特性的比较[J]．扬州大学学报: 农业与生命科学版， 2009， 30(1) : 78 - 83．
[8] 孙庆泉，吴元奇，胡昌浩，等．不同产量潜力玉米籽粒胚乳细胞增殖与籽粒充实期的生理活性[J]．作物学报， 2005， 31(5) : 612 - 618．
[9] Yang J，Zhang J． Grain-filling problem in super rice[J]．Journal of Experimental Botany， 2010 ， 61(1) : 1 - 5．
[10] Sheng J H， Jiang B，Ming Y D， et al． Simultaneous determination of gibberellic acid， indole-3-acetic acid and abscisic acid in wheat extracts by solid-phase extraction and liquid chromatography electrospray tandem mass spectrometry[J]． Talanta， 2008 ， 76: 798 - 802．
[11] 荣湘民，刘强，朱红梅．水稻的源库关系及碳、氮代谢的研究进展[J]．中国水稻科学，1995，12( 增刊) :63 - 69．
[12] Pérez-Alfocea F，Albacete A，Ghanem M， et al． Hormonal regulation of source-sink relations to maintain crop productivity under salinity: a case study of root-to-shoot signalling in tomato[J]． Functional Plant Biology， 2010， 37(7) : 592 - 603 ．
[13] 梁建生，曹显祖，张海燕，等．水稻籽粒灌浆期间茎鞘贮存物质含量变化及其影响因素研究[J]．中国水稻科学， 1994，8(3) : 151 - 156．
[14] Wang F，Chang F，Zhang G ． The relationship between grain filling and hoemone content as affected by genotype and source-sink relation[J]． Plant Growth Regulation，2006， 49(1) : 1 - 8．
[15] 杨建昌，彭少兵，顾世梁，等．水稻结实期籽粒和根系中玉米素与玉米素核苷含量的变化及其与籽粒充实的关系[J]．作物学报， 2001， 21(7) : 36 - 42．
[16] Jin D，Wang W，Sheng L， et al． Dynamic status of endogenous IAA，ABA and GA levels in superior and inferior spikelets of heavy panicle hybrid rice during grain fillin[J]． Journal of Plant Physiology and Molecular Biolog ，2002， 28(3) : 215 - 220．
[17] 董明辉，赵步洪，吴翔宙，等．水稻结实期不同粒位籽粒相关内源激素含量和关键酶活性的差异及其与品质的关系[J]．中国农业科学，2008，41 (2) : 370 -380．
[18] 黄锦文，梁康迳，梁义员．不同类型水稻籽粒灌浆过程内源激素含量变化的研究[J]．中国生态农业学报， 2003， 11(2) : 11 - 13．
[19] 阿加拉铁，薛大伟，李仕贵，等．植物激素与水稻产量的关系[J]．发展与对策， 2006，5: 1 - 3．
[20] Yang J，Zhang J，Wang Z， et al． Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene[J]． Journal of Experimental Botany ， 2006， 57，(1) : 149 - 160．
[21] Yang J，Zhang J，Wang Z， et al． Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice[J]． Plant Growth Regulation， 2003，41: 185 - 195．
[22] 潘瑞炽．植物生理学[M]．北京: 高等教育出版社，2008: 67 - 194．
[23] 刘琳，吕文彦，呼楠，等．粳稻胚乳细胞增殖与蔗糖代谢的关系[J]．植物生理学报，2011，47 (10) :1003 - 1009．

选择文件类型/文献管理软件名称

选择包含的内容