华北农学报 ›› 2013, Vol. 28 ›› Issue (1): 144-148. doi: 10.3969/j.issn.1000-7091.2013.01.027

所属专题: 小麦

• 论文 • 上一篇    下一篇

冬小麦叶片对低温胁迫的生理响应

衣莹1,2, 张玉龙1, 郭志富3, 白丽萍3, 乔雪3, 侯立白4   

  1. 1. 辽宁省农业资源与环境重点实验室,沈阳农业大学土地与环境学院,辽宁沈阳110866;
    2. 沈阳农业大学农学院,辽宁沈阳 110866;
    3. 沈阳农业大学生物科学技术学院,辽宁沈阳 110866;
    4. 沈阳农业大学科学技术学院,辽宁抚顺 113122
  • 收稿日期:2012-11-20 出版日期:2013-02-28
  • 通讯作者: 张玉龙(1954-),男,辽宁建平人,教授,博士,主要从事土壤改良与农业节水研究。郭志富(1980-),男,内蒙古赤峰人,讲师,博士,主要从事小麦族物种功能基因发掘与克隆研究。
  • 作者简介:衣莹(1971-),女,内蒙古赤峰人,讲师,博士,主要从事小麦抗逆生理研究。
  • 基金资助:
    国家自然科学基金项目:强冬性新型抗冻基因TalRl3和TalRl4的克隆及功能研究(30900894);总理基金:冬麦北移试验示范开发项目(dmby96-03);沈阳农业大学校内基金:强冬性小麦抗寒性鉴定(413010101-110901-01025010005)

Physiological Responses of WinteR Wheat's Leaves to Low TempeRatuRe StRess

YI Ying1,2, ZHANG Yu-long1, GUO Zhi-fu3, BAI Li-ping3, QIAO Xue3, HOU Li-bai4   

  1. 1. Key LaboRatoRy of AgRicultuRal ResouRce and EnviRonment Liaoning PRovince,College of Land and EnviRonmental Science,Shenyang AgRicultuRal UniveRsity,Shenyang 110866,China;

    2. College of AgRonomy,Shenyang AgRicultuRal UniveRsity,Shenyang 110866,China;

    3. College of Biological Science and Technology,Shenyang AgRicultuRal UniveRsity,Shenyang 110866,China;

    4. Shenyang AgRicultuRal UniveRsity,College of Science and Technology, Fushun 113122,China
  • Received:2012-11-20 Published:2013-02-28

摘要: 以抗寒性不同的冬小麦品种(米808、冬引0801、小黑麦、冬麦9625、冬麦138)为试验材料,测定各材料在不同温度(13,7,4,-18,-21℃)下处理2 h后叶片的相对电导率、叶片中丙二醛(MDA)、可溶性蛋白质、脯氨酸、可溶性糖含量和超氧化歧化酶的活性(SOD)。结果表明,随着温度降低(4℃→-18℃→-21℃),小麦叶片中MDA、可溶性糖含量逐渐增加。小麦叶片SOD总活力随胁迫温度降低先升高后降低再升高。经低温胁迫后,小麦叶片可溶性蛋白质含量无明显变化。米808、冬引0801在-21℃胁迫下,相对电导率变化率最低。米808、冬引0801在-18℃→-21℃时,MDA含量增幅较大;米808 SOD总活力在-18、-21℃时变化率最低。冬引0801脯氨酸和可溶性糖的变化率均在21℃时最大。

关键词: 冬小麦, 低温胁迫, 生理生化特性

Abstract: DiffeRent cool-Resistance vaRieties weRe used to expeRimental mateRial to test Relative conductivity, malondialdehyde, soluble pRotein,pRoline, soluble sugaR content and the activities of supeRoxide dismutase afteR 2 houRs tReatment with 13,7 ,4 ,- 18,- 21 ℃. As the tempeRatuRe was Reduced,MDA and soluble sugaR content of wheat leaf incRease gRadually, the activities of SOD tRend is N-shaped wave. AfteR low tempeRatuRe stRess, the soluble pRotein content of wheat leaves no significant change. The Results indicated that M808 and Dongyin0801 have the minimum Relative electRic conductivity in 21 ℃. MDA content of M808 and Dongyin0801 incReased consideRably fRom - 18 ℃ to - 21 ℃. M808's activities of SOD has the lowest Rate of change in - 18 ℃ and - 21 ℃. PRoline and soluble sugaR content of Dongyin0801 has the laRgest Rate of change in - 21 ℃.

Key words: WinteR wheat, Low tempeRatuRe stRess, Physiological and biochemical chaRacteRstics

中图分类号: 

引用本文

衣莹, 张玉龙, 郭志富, 白丽萍, 乔雪, 侯立白. 冬小麦叶片对低温胁迫的生理响应[J]. 华北农学报, 2013, 28(1): 144-148. doi: 10.3969/j.issn.1000-7091.2013.01.027.

YI Ying, ZHANG Yu-long, GUO Zhi-fu, BAI Li-ping, QIAO Xue, HOU Li-bai. Physiological Responses of WinteR Wheat's Leaves to Low TempeRatuRe StRess[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 144-148. doi: 10.3969/j.issn.1000-7091.2013.01.027.

使用本文