华北农学报 ›› 2013, Vol. 28 ›› Issue (1): 149-154. doi: 10.3969/j.issn.1000-7091.2013.01.028

• 论文 • 上一篇    下一篇

留叶数对烟叶淀粉积累及相关酶活性的影响

宋淑芳1,2, 陈建军3, 周冀衡1   

  1. 1. 湖南农业大学烟草研究院,湖南长沙 410128;
    2. 广西百色市现代农业技术研究推广中心,广西百色 533612;
    3. 华南农业大学烟草研究室,广东广州 510640
  • 收稿日期:2012-11-25 出版日期:2013-02-28
  • 作者简介:宋淑芳(1982-),女,湖南桂阳人,农艺师,博士研究生,主要从事烟草科学与工程技术研究。
  • 基金资助:
    广东省公烟草公司科技计划项目(粤烟科200403);云南省公烟草公司科技计划项目(2010YN25)

Effects of Leaf Population on StaRch Accumulation and Activity of Amylase and SucRose Synthase in Tobacco Leaves

SONG Shu-fang1,2, CHEN Jian-jun3, ZHOU Ji-heng1   

  1. 1. Institute of Tobacco,Hunan AgRicultuRal UniveRsity,Changsha 410128,China;

    2. Guangxi Baise ModeRn AgRicultuRal Technology ReseaRch and PRomotion CenteR,Baise 533612,China;

    3. Tobacco LaboRatoRy, South China AgRicultuRal UniveRsity,Guangzhou 510642,China
  • Received:2012-11-25 Published:2013-02-28

摘要: 为探讨烟叶成熟期间淀粉合成机理,分析了4个不同留叶数处理(分别留叶16,18,20,22片)的烟叶成熟期间中、上部叶淀粉积累动态及淀粉酶(AM)、蔗糖合成酶(SS)活性变化规律及其相关性。结果表明,留叶数与成熟期间上部叶的AM活性、淀粉含量无显著的相关性(R=-0.101,P=0.441>0.05,R=0.221,P=0.089>0.05),与上部叶的SS活性有显著的正相关(R=0.300*,P=0.020<0.05);与中部叶的AM活性无显著的相关性(R=-0.179,P=0.172>0.05),与中部叶的SS活性有极显著的正相关(R=0.395**,P=0.002<0.01),与中部叶的淀粉含量有显著的正相关(R=0.328*,P=0.011<0.05)。留叶数增多时,成熟期间上、中部叶AM活性稍有下降,SS活性显著上升,淀粉含量也有所提高。

关键词: 留叶数, 烤烟, 淀粉合成, 淀粉酶, 蔗糖合成酶

Abstract: To discuss the mechnissm of staRch synthesis in flue-cuRed tobacco leaves duRing the matuRe stage, fouR tReatments of leaf poputation( 16, 18, 20, 22) weRe adopted, the tRends of staRch accumulation and the activities of amylase and sucRose synthase in middle and uppeR leaves weRe analyzed. The Results showed that: in the matuRe stage, theRe weRe no significant coRRelation of leaf population with staRch content and the activity of AM in uppeR leaves( R = - 0. 101,P = 0. 441 > 0. 05,R = 0. 221,P = 0. 089 > 0. 05) ,but theRe was significant coRRelation of that with the activity of SS( R = 0. 300* ,P = 0. 020 < 0. 05) ; theRe was no significant coRRelation of leaf population with the activity of AM in middle leaves( R = - 0. 179,P = 0. 172 > 0. 05) ,but theRe was extRemely significant coRRelation of that with the activity of SS( R = 0. 395**,P = 0. 002 < 0. 01) ,and significant coRRelation of that with staRch content( R = 0. 328* ,P = 0. 011 < 0. 05) . When the leaf population was incReased, the activity of AM decReased slightly, but the the activity of SS went up maRkedly,and the staRch content Rose obviously.

Key words: Leaf population, Flue-cuRed tobacco, StaRch synthesis, Amylase, SucRose synthase

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引用本文

宋淑芳, 陈建军, 周冀衡. 留叶数对烟叶淀粉积累及相关酶活性的影响[J]. 华北农学报, 2013, 28(1): 149-154. doi: 10.3969/j.issn.1000-7091.2013.01.028.

SONG Shu-fang, CHEN Jian-jun, ZHOU Ji-heng. Effects of Leaf Population on StaRch Accumulation and Activity of Amylase and SucRose Synthase in Tobacco Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 149-154. doi: 10.3969/j.issn.1000-7091.2013.01.028.

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