摘要： 为了研究日粮添加L-精氨酸对环江香猪营养物质消化率、血浆生化参数及游离氨基酸浓度的影响，选取10头平均体重为18．9±2．4kg的环江香猪，随机分为2组，每组5头，单笼饲养，分别饲喂添加0．83％精氨酸的基础日粮（试验组）和1．71％丙氨酸的基础日粮（等氮对照组），试验期为7d。每天记录试猪的采食量，并于试验开始和结束时分别称取空腹体重；试验第5～7d收集粪样，用TiO2指示剂法测定常规营养成分的消化率；试验结束后空腹采集血液，肝素抗凝，离心分离血浆，测定其生化参数和游离氨基酸浓度。结果表明，与丙氨酸组相比，精氨酸组试猪平均日增重有升高趋势、料重比有降低趋势（P〉0．05），干物质消化率显著增加（P〈0．05）；血氨浓度显著降低、总蛋白浓度显著升高（P〈0．05），血浆中天冬氨酸、谷氨酸和苏氨酸浓度显著升高（P〈0．05），丙氨酸浓度显著降低（P〈0．05），总氨基酸浓度、支链氨基酸／芳香族氨基酸值和必需氨基酸／非必需氨基酸值均有增加趋势（P〉0．05）。上述结果提示，在日粮中添加适量精氨酸可增强环江香猪对某些日粮营养素的消化吸收，提高机体中一些必需氨基酸的含量，有利于机体蛋白质的合成，保证了其健康生长。
关键词:
精氨酸,
环江香猪,
生化参数,
氨基酸
Abstract: This study was conducted to determine the effects of dietary supplementing arginine on nutrient di- gestibility,plasma biochemical parameters and concentrations of free amino acids （AA） in Huanjiang mini-pigs. （Method）Ten Huanjiang mini-pigs with body weight （BW） of （18.9 ±2.4） kg were used and randomly assigned into 2 groups （5 replicates with one pig per group）,representing supplementation with 0.83% arginine （experi- ment group） or 1.73% alanine （isonitrogenous control group） in a basal diet for 7 days. Daily feed intake,initial and final empty stomach BW were recorded,respectively;fecal samples were collected from day 5 to 7 for determi- ning the digestibility of routine nutrient by TiO2 indicator;blood samples were collected into heparin-coated tubes at the end of the trial for analyzing biochemical parameters and concentrations of free AA, respectively. （Result） Re- suits showed that argiuine supplementation increased the average daily gain and ratio of gain to feed （ P 〉 0.05 ）, as well as the apparent digestibilities of dry matter（P 〈0.05） and crude protein（P 〉0.05）,compared with the con- trol group;the plasma concentrations of AMM and alanine in pigs fed arginine-supplemented diet decreased （ P 〈 0.05 ）,while of the total protein, aspartate, glutamate and threonine increased （ P 〈 0.05 ）, as well as the total AA, branched chain AA/Aromatic AA, essential AA/nonessential AA （ P 〉 0. 05 ）, in comparison with the control group. （Conclution）These findings suggested that dietary supplementing arginine could increase digestion and ab- sorption of some nutrients,improve contents of some essential AA, which resulted in the increased protein synthesis and healthy growth of Huanjiang mini-pigs.
Key words:
Arginine,
Huanjiang mini-pig,
Biochemical parameters,
Amino acids
中图分类号:
周笑犁, 汤文杰, 孔祥峰, 印遇龙, 耿梅梅. 精氨酸对环江香猪营养物质消化率和血液指标的影响[J]. 华北农学报, 2012, 27(4): 234-238. doi: 10.3969/j.issn.1000-7091.2012.04.045.
ZHOU Xiao-li, TANG Wen-jie, KONG Xiang-feng, YIN Yu-long, GENG Mei-mei. Effects of Arginine on Nutrient Digestibility and Blood Parameters in Huanjiang Mini-pigs[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 234-238. doi: 10.3969/j.issn.1000-7091.2012.04.045.