摘要： 研究硅对盐胁迫下黄瓜幼苗生长及根细胞质膜H⁺－ATPase、液泡膜H⁺－ATPase、H⁺－PPase和Ca²⁺－ATPase活性的影响。结果表明：盐胁迫严重抑制黄瓜幼苗生长，根细胞质膜H⁺－ATPase、液泡膜H⁺－ATPase、H⁺－PPase和Ca²⁺－ATPase活性明显降低。硅处理明显提高盐胁迫黄瓜根液泡膜H⁺－ATPase、H⁺－PPase、Ca²⁺－ATPase活性，一定程度上维持了液泡膜质子泵活性，有效地防御了细胞质酸化，这可能是硅提高黄瓜耐盐性的一个重要因素。

关键词: 盐胁迫, 黄瓜, 硅, 质膜H⁺－ATPase, 液泡膜H⁺－ATPase, 液泡膜H⁺－PPase, Ca²⁺－ATPase

Abstract: The effects of exogenous Si on the changes of activities of the plasma membrane H⁺-ATPase and tonoplast H⁺-ATPase,H⁺-PPase and Ca²⁺-ATPase were investigated in roots of salt-stressed cucumber seedlings(Cucumis sativus L.).The results showed that salt stress significantly decreased the plant growth and the activities of plasma membrane H⁺-ATPase and tonoplast H⁺ATPase,H⁺-PPase and Ca²⁺-ATPase.Addition of Si markedly increased the activities of plasma membrane H⁺-ATPase and tonoplast H⁺-ATPase,H⁺-PPase and Ca²⁺-ATPase under salt stress,maintained the activety of proton pumping of tonoplast to some extent and defensed effectively the cytoplasmic acidizing,which may be the important factor for improving the salt tolerance of cucumber by Si.

Key words: Salt stress, Cucumber(Cucumis sativus L.), Silicon, Plasma membrane H⁺-ATPase, Tonoplast H⁺-ATPase, Tonoplast H⁺-PPase, Ca²⁺-ATPase

中图分类号: 

• S143.7+1

• S642.201