摘要： 枯萎病拮抗菌B96-II（Bacillussp）自沤肥浸渍液中分离筛选获得。进一步试验证明，该菌对黄瓜枯萎病菌、西瓜枯萎病菌、青椒枯萎病菌等有明显抑制效果。可观察到的抑菌现象有：对孢子的溶解作用；对孢子壁的破坏作用；对芽管的生长抑制和对菌丝致畸等作用，对上述3种病菌孢子萌发抑制率为56.2%，57.3%，54.7%，对芽管的抑制率分别为88.5%，87.5%，79.5%。拮抗物质之一是几丁质酶。
关键词:
枯萎病,
尖孢镰刀菌,
拮抗菌,
抑制作用,
几丁质酶
Abstract: A antagonistic strain of Bacillus sp(B96-II) against Fusarium wilt was isolated from compost extract.Further tests proved that the B96-II could effectively inhibit the development of 3 Fusarium wilts in cucumber,water melon,paprika,etc.The phenomena of conidia lysogenesis,conidia wall destruction,inhibition,and deformation of hyphal growth were observed.The inhibition rates of conidia germination of the above mentioned pathogens were 56.2%,57.3%,and 54.7%,and the inhibition rates of germination tube growth were 88.5%,87.5%,and 79.5%.Chitinase was detected as one of the main inhibiting substances.
Key words:
Soil born disease,
Fusarium oxysporum,
Antagonist,
Chitinase,
Lysogenesis
中图分类号:
马利平, 乔雄梧, 高芬, 郝变青. B96-II对3种枯萎病的防治效果及拮抗物质初步分析[J]. 华北农学报, 2006, 21(4): 99-102. doi: 10.3321/j.issn:1000-7091.2006.04.025.
MA Li-ping, QIAO Xiong-wu, GAO Fen, HAO Bian-qing. Inhibition Effects of Antagonist Stain B96-Ⅱ to Different Fusarium Wilt and Its Main Inhibiting Substance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(4): 99-102. doi: 10.3321/j.issn:1000-7091.2006.04.025.