摘要： 为高效获得葡萄霉菌病生防菌菌株，以病原真菌细胞壁的关键组分几丁质和β-1，3-葡聚糖为唯一碳源，通过底物压力筛选法，选择性地富集同时具有几丁质酶活和β-1，3-葡聚糖酶活的目的菌株。结果表明，此筛选方法有效地富集了潜在的目标菌株。挑取的10株菌均具有几丁质酶活和β-1，3-葡聚糖酶活。其中，1号菌株单位菌体几丁质酶活和单位菌体β-1，3-葡聚糖酶活分别达到2.52，0.76 U/mL；6号菌株的则分别为1.45，3.99 U/mL。酶学研究证明2株菌的酶均为诱导型，模拟自然条件下，2株菌在培养24或36 h酶活最高。经16S rDNA测序鉴定，2株菌均为假单胞菌，是一种极具生防潜力的内生细菌。

关键词: 葡萄霉菌病, 生防菌, 筛选压力, 几丁质酶, β-1,3-葡聚糖酶

Abstract: The extension of bio-control bacteria resources is significant to the development of bio-control of grape mycosis. To efficiently acquire bio-control bacteria again grape mycosis,the key components of the cell wall of fungal pathogen,chitin and β-1,3-gluncan,were successively used as the only carbon source. Through the screening method with substrate stress,target strains with both chitinase activity and β-1,3-glucanase activity were selectively enriched. Results suggested that this screening method effectively enriched the potential target strains. All of the 10 picked strains demonstrated chitinase activity and β-1,3-glucanase activity. Among them,the chitinase activity per biomass and β-1,3-glucanase activity per biomass of strain 1# reached 2.52,0.76 U/mL respectively,and those of strain 6# reached 1.45,3.99 U/mL respectively. Enzyme study proved that the enzymes of both strains were inducible. Under simulated natural conditions,the enzyme activities of the two strains reached the highest at 24 or 36 h. Through 16S rDNA sequencing,both of the two strains were identified as Pseudomonas sp.,which is a kind of endophytic bacteria with great potential for bio-control.

Key words: Grape mycosis, Bio-control bacteria, Screening stress, Chitinase, β-1,3-glucanase

中图分类号: 

• S432.4

• Q939.92