摘要： 以美国红葡萄、黑葡萄为供试材料，对葡萄试管苗快繁过程中，从取材、材料消毒、诱导茎芽萌发、增殖、生根到移栽这几个关键环节进行了研究。试验表明，在葡萄旺盛生长的6，7，8月份取材，以侧芽做外植体比顶芽易存活；材料用75%酒精处理20s，再用0.1%升汞浸泡4 min，存活率可达70%~85%。6-BA浓度为0.5mg/L时诱导芽增殖萌发效果最佳，每4周可增殖3~4倍。在生根培养基中NAA浓度为0.2mg/L时，生根率达90%左右。分步移栽，可使移栽成活率达80%。

关键词: 葡萄, 试管苗, 存活率, 增殖, 根诱导, 移栽

Abstract: Experiments were conducted to study various factors affecting rapid propagation of grape (America red grape and America black grape) in vitro. Selection and sterilization of plant material, induction of shoot bud and multiple shoots, shoot rooting and plant transplanting were all studied. The original shoots from grape in June, July and August showed good potential for culture. The survival rate of axillary buds was higher than that of the apical buds. By dipping the original shoots in 75% ethanol for 20 seconds, and then treating with 0.1% mercuric chloride for4 minutes, the survival rate of shoot buds was up to 70%-80%. The 0.5mg/L6-BA solution significantly stimulated budding and shoots elongation, the propagation coefficient was3-4 times per week. Shoot rooting rate reached 90% when shoots were planted in the root-induction medium NAA (0.2mg/L). The survival rate of transplanted plants was around 80%, when they were conducted step by step.

Key words: Grape, Rapid propagation in vitro, Survival rate, Induction of multiple shoots, Induction of rooting, Transplant