摘要： 对绿菜花“Galaxy”、“bc-0”和“bc-4”3个基因型进行了渗透压、外源激素、热激处理温度和时间、花蕾长度或小孢子发育时期的影响因素研究。结果表明，激素的作用并不明显，但相比之下，单独加入6-BA比同时加入6-BA、NAA效果好；绿菜花游离小孢子培养的最适热激处理温度和时间为32.5℃，24h;绿菜花游离小孢子的最适培养花蕾长度因基因型而异，如基因型“bc-4”蕾长为4.1~5.0mm,此时大多数小孢子处在单核晚期、双核早期，出胚率高达37.08胚/蕾。

关键词: 绿菜花, 小孢子培养, 影响因素

Abstract: In the study, 3 genotypes of broccoli, i.e.“Galaxy"、“bc-0”、“bc-4”were used to study factors such as osmotic pressure, exo-hormones, the temperature and time of heat treatment and the length of bud or the stage of pollen development which effect on the isolated microspore culture of broccoli.The exo-hormones were not essential for inducing embryogenesis, but 6-BA alone could produce better results than 6-BA plus NAA.The heat treatment at 32.5℃ for 24hr was the most effective for inducing embryogenesis.The most effective length of buds was dependent on plant genotypes.For example, for “bc-4”at the period of buds 4.1-5.0mm in length, most of the pollens belonged to the late-uninucleate and the early-binucleate stages, it produced 37.08 embryos per bud.

Key words: Broccoli, Isolated microspore culture, Effect factors