华北农学报 ›› 1997, Vol. 12 ›› Issue (3): 120-124. doi: 10.3321/j.issn:1000-7091.1997.03.023

所属专题: 萝卜 蔬菜专题

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用反相高压液相色谱法对不同品种胡萝卜中β-胡萝卜素含量的测定

刘玲, 金同铭   

  1. 北京蔬菜研究中心, 北京 100081
  • 收稿日期:1995-10-05 出版日期:1997-09-28

Determination of β-carotene in Different Carrot Varieties by Reversed Phase High Pressure Liquid Chromatography

Liu Ling, Jin Tongming   

  1. Beijing Vegetable Research Center, Beijing 100081
  • Received:1995-10-05 Published:1997-09-28

摘要: 应用反相高压液相色谱法(RP-HPLC),经Nova-PakC18柱进行梯度洗脱,在454nm处检测。测定29个不同品种胡萝卜中β-胡萝卜素含量,其总平均值为3.56mg·100g-1.英国品种Nantura含量最高为6.92mg·100g-1;4个品种中有2个品种β-胡罗卜素含量超过6.0mg·100g-1,接近总平均值的2倍。中国和法国的品种β-胡萝卜素的含量相对较低,中国4个品种的总平均仅为测试样品总平均值的56%,而法国的B180品种含量用本实验方法无法检出,含量低于7ng·100g-1.

关键词: 胡萝卜, &beta, -胡萝卜素, 反相高压液相色谱

Abstract: Reversed phase high pressure liquid chromatography (RP HPLC) in 454nm was used to determine the content of β carotene for 29 different carrot varieties,and the total average content was found to be 3.56mg·100g-1 .β carotene content of nantura from U.K. was the highest,6.92mg·100g-1 of U.K. varieties,2 exeeded 6.0mg·100g-1 which were 2 times of the total average. Chinese and French varieties were relatively low.The total average oF 4 Chinese varieties was only 56% of that of the samples,while French B180 was the lowest,lower than 7ng·100g-1 which could not be detected by this method.

Key words: Carrot, β, -carotene, Reversed phase high pressure chromatography

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引用本文

刘玲, 金同铭. 用反相高压液相色谱法对不同品种胡萝卜中β-胡萝卜素含量的测定[J]. 华北农学报, 1997, 12(3): 120-124. doi: 10.3321/j.issn:1000-7091.1997.03.023.

Liu Ling, Jin Tongming. Determination of β-carotene in Different Carrot Varieties by Reversed Phase High Pressure Liquid Chromatography[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1997, 12(3): 120-124. doi: 10.3321/j.issn:1000-7091.1997.03.023.