华北农学报 ›› 2013, Vol. 28 ›› Issue (2): 42-45. doi: 10.3969/j.issn.1000-7091.2013.02.008

所属专题: 玉米

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Curvularia lunata侵染不同抗性玉米品系的应答差异蛋白解析

夏淑春1, 张茹琴1, 王琰2, 鲁闽3, 鄢洪海1   

  1. 1. 青岛农业大学 农学与植物保护学院, 山东 青岛 266109;
    2. 山东省乳山市农业局, 山东 威海 264500;
    3. 烟台出入境检疫检验局, 山东 烟台 264000
  • 收稿日期:2012-12-06 出版日期:2013-04-28
  • 通讯作者: 鄢洪海(1964-),男,吉林九台人,教授,博士,主要从事植物病理生理与分子生物学研究。
  • 作者简介:夏淑春(1964-),女,辽宁锦州人,副教授,主要从事植物有害生物综合治理研究。
  • 基金资助:
    山东省科技发展项目(2009GG10009022);山东省自然科学基金项目(ZR2011CL005);山东省“泰山学者”建设工程专项经费资助(BS2009NY040)

Proteome Changes in Corn Varieties QI319 and 7922 after Inoculating Curvularia lunata

XIA Shu-chun1, ZHANG Ru-qin1, WANG Yan2, LU Min3, YAN Hong-hai1   

  1. 1. Qingdao Agriculturd University, College of Agronomy and plant protection Qingdao 266109, China;
    2. Agriculture Bureau of Rushan City, Weihai 264500, China;
    3. Yantai Entry-Exit Inspection and Quarantine Bureau, Yantai 264000, China
  • Received:2012-12-06 Published:2013-04-28

摘要: 用山东莱西市玉米弯孢叶斑病菌强致病类型菌株QN-10,接种玉米抗病自交系齐319和感病自交系7922,采用蛋白质组技术分析其差异蛋白及功能。结果表明,齐319感染弯孢菌3 d后,有12个差异蛋白质斑点被诱导合成,8个为增量表达蛋白质斑点,2个为减量表达蛋白质斑点,2个为新产生蛋白质斑点|自交系7922有3个蛋白质斑点表达量增加,2个斑点表达量下降,但没有发现新的蛋白质合成。齐319差异蛋白质斑点鉴定表明,分别属于β-1,3-葡聚糖酶、F-box亮氨酸高度重复蛋白、锌指蛋白。

关键词: 弯孢叶斑病菌, 玉米, 蛋白质组, &beta, -1, 3-葡聚糖酶

Abstract: The protein of resistant corn variety QI 319 and susceptible variety 7922 was extracted from leaves and separated using two-dimensional polyacrylamidgel electrophoresis(2-D)at 3d after inoculating prevalent race QN-10 of Curvularia lunata. In QI 319,compared with control with no pathogen inoculation,12 novel proteins were induced;In 7922,three protein spots of and two protein spots of showed increase and decrease in protein abun-dance,respectively,but there were no hovel protein spots induced. Among the 12 hovel protein spots induced in QI 319,six spots were identified using MALDI-TOF-MS and NCBI database searching,which were F-box and leucine-rich repeat protein,heavy metal transport/detoxification protein,beta-1,3-glucanase and zinc finger protein.

Key words: Curvularia lunata, Maize, Proteome, β-1, 3-glucanase

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引用本文

夏淑春, 张茹琴, 王琰, 鲁闽, 鄢洪海. Curvularia lunata侵染不同抗性玉米品系的应答差异蛋白解析[J]. 华北农学报, 2013, 28(2): 42-45. doi: 10.3969/j.issn.1000-7091.2013.02.008.

XIA Shu-chun, ZHANG Ru-qin, WANG Yan, LU Min, YAN Hong-hai. Proteome Changes in Corn Varieties QI319 and 7922 after Inoculating Curvularia lunata[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 42-45. doi: 10.3969/j.issn.1000-7091.2013.02.008.

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