摘要： 促卵泡素是动物垂体前叶分泌的重要繁殖调控激素。采用PCR产物直接测序法对河流型和沼泽型水牛、牦牛和大额牛的FSHβ基因外显子3编码区进行群体变异检测,并进行了生物信息学分析。牛科物种FSHβ基因外显子3编码区均为231 bp。在水牛中共发现16个SNP位点,其中异义替换13个,推测4个异义替换对FSH功能有显著影响(subPSEC<|-3)。沼泽型水牛中发现的SNP位点均与河流型水牛共享,但它们在两类水牛中的群体遗传组成不同。在普通牛、牦牛和大额牛中分别确定8,3,1个SNP位点,但只有SNP307是异义替换,普通牛与牦牛共享该位点,推测其对FSH功能无显著影响(subPSEC>|-3)。在山羊中有11个SNP位点,8个为异义替换,推测只有SNP253对FSH功能有显著影响(subPSEC<|-3)。在绵羊中有3个SNP位点,皆为同义替换。确定水牛特有的核苷酸位点4个,山羊特有的位点1个,普通牛、牦牛和大额牛共有的核苷酸位点4个,山羊和绵羊中共有的位点2个,这些位点编码氨基酸的差异可能引起了不同类型牛科物种间FSHβ亚基功能上的差异性。

关键词: 牛科物种, 促卵泡素&beta, 亚基基因, 外显子3, 多态性, 生物信息学分析

Abstract: Follicle stimulating hormone(FSH) has important effects in regulating animal reproductive function. In this paper,the polymorphisms in the coding region of FSHβgene exon 3 of river buffalo,swamp buffalo,yak andgayal were detected by using PCR with direct sequencing. The data obtained in this study and published about bo-vine species were dealt with by employing the methods of bioinformatics. The coding region in FSHβgene exon 3 contained 231 bp in all the species of Bovidae. In buffalo,we identified 16 SNPs,of which 13 were non-synony-mous,and 4 non-synonymous loci were predicted to have significant effect on FSH function(subPSEC ＜-3). The SNP loci detected in swamp buffalo were all shared with river buffalo,but the populationgenetic composition were different in two types of buffalos. There were 8,3 and 1 SNPs in cattle,yak andgayal,respectively,but only SNP307 was non-synonymous which was shared by yak and cattle and was predicted to have no significant effect on FSH function(subPSEC ＞-3). Ingoat,11 SNPs were found,of which 8 SNPs were non-synonymous,but only SNP253 was predicted to have significant effect on FSH function(subPSEC ＜-3). As for sheep,3 SNPs were found,they are non-synonymous. In this paper,there are 4 and 1 specific nucleotide sites identified in buffalo andgoat,respectively. There are 4 shared nucleotide sites identified among cattle,yaks andgayal,and 2 shared nucleotide sites identified be-

中图分类号: 

• S823. 8

• Q75