摘要： 0.5MPaPEG-6000胁迫24h明显刺激三叶期玉米幼苗，第三叶质膜ATPase活性提高，并且V_max增加近一倍，而K_m值却降低；水分胁迫下渗透调节因子脯氨酸累积，K含量则呈现先上升后下降的趋势，渗透调节的起动与质膜ATPase活性上升可能无直接关系。实验也表明质膜ATPase活性被三氟拉嗪（TFP）促进而受钙调素抑制，由此推断质膜ATPase在钙调素参与的磷酸化后活性降低。

关键词: 玉米, 幼叶, 质膜, 水分胁迫, 钙调素, 渗透调节

Abstract: The PM ATPase activity of the third maize leaves significantly increased in the three leaves seedlings under 0.5MPa PEG 6000 for 24 hours,and V_max rised almost one time,but K_m decreased.With the development of water stress,proline accumulated, treatment of vanadate, a inhibitor of ATPase,delay proline accumulation 24 hours. Total contents rised,then declined with or without vanadate. These suggested that the start of osmoregulation may be not related with the increasing of ATPase activities. Trifluoperazine,a inhibitor of calmodulin, stimulated calmodulin and inhibited PM ATPase activities. It suggested that ATPase activities may be declined after phosphorylation.

Key words: Maize leaves, Plasma membrane, ATPase, Water stress, Calmodulin, Osmoregulation

中图分类号: 

• S513.01