华北农学报 ›› 1996, Vol. 11 ›› Issue (4): 119-122. doi: 10.3321/j.issn:1000-7091.1996.04.024

所属专题: 甜椒辣椒

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过氧化物酶及种子蛋白分析鉴定甜椒杂交种纯度

郑晓鹰, 黄为平   

  1. 北京蔬菜研究中心, 北京 100081
  • 收稿日期:1996-02-02 出版日期:1996-12-28

Purity Testing of Sweet Pepper (Capsicum annuum L) F 1 Varieties by Peroxidase Isozyme and Water Soluble Protein Analysis

Zheng Xiaoying, Huang Weiping   

  1. Beijing Vegetable Research Center, Beijing 100081
  • Received:1996-02-02 Published:1996-12-28

摘要: 用聚丙烯酰胺均匀胶和等电聚焦电泳技术,分析了甜椒种子及种苗的过氧化物同功酶谱和种子水溶蛋白图谱。在过氧化物酶谱带的两个位点PRX-1和PRX-2上,自交系亲本与F1杂交种有明显的差异。水溶蛋白的一个位点PROT亲本与F1有明显差异。供试甜椒组合的过氧化物酶和蛋白的图谱,分别具有这些位点。利用这些鉴别图谱,对甜椒商品种子样品进行的杂交种纯度检验,纯度范围在8%~99%,与田间检测结果一致。这一电泳检测技术可以有效地用于甜椒杂交种纯度的检验。

关键词: 甜椒, 同功酶, 水溶蛋白, 纯度鉴定

Abstract: Peroxidase isozyme phenotypes and water soluble protein were determined in parental inbreds and corresponding F 1 hybrid seed lots of two commercial sweetpepper varieties to determine seed purity .The inbred lines differed with their F 1 hybids at two peroxidase loci (PRX-1, PRX-2) and one water soluble protein locus(PROT).Differences between inbred parents were found at least for one of the three loci.Models were developed to estimate seed genetic purity. The genetic purity was estimated range from 8% to 99%.These estimates conformed favorably with those obtained from field testing.

Key words: Sweet pepper, Isozyme, Water soluble protein, F 1 hybrid purity identification

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引用本文

郑晓鹰, 黄为平. 过氧化物酶及种子蛋白分析鉴定甜椒杂交种纯度[J]. 华北农学报, 1996, 11(4): 119-122. doi: 10.3321/j.issn:1000-7091.1996.04.024.

Zheng Xiaoying, Huang Weiping. Purity Testing of Sweet Pepper (Capsicum annuum L) F 1 Varieties by Peroxidase Isozyme and Water Soluble Protein Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1996, 11(4): 119-122. doi: 10.3321/j.issn:1000-7091.1996.04.024.

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