摘要： 对不同来源的10个品种的黄瓜种子低温下萌发研究结果表明，种子萌发最敏感的低温是13～15℃。在此温度范围内，不同品种黄瓜种子发芽率有显着差异。低温萌发过程中，POD活性均呈曲线变化，有一高峰出现，并且按高峰出现时间的早晚划分成3种类型。高峰出现早的品种，其种子发芽率高，萌发速度快；反之，发芽率低，萌发速度慢。低温抑制了POD活性，减少了POD同功酶条带和强度。

关键词: 黄瓜, 低温萌发, POD, 同功酶

Abstract: This research concerns the most desirable temperature for selecting chilling-tolerant cucumber at the stage of germination, and the effects of low temperature on germination. Ten cucumber breeding lines were tested for germination and the activities of peroxidase and its isozymes at different temperatures. The results showed that there were significant genetic differences in germination at 13-15℃. The two lines that had the best germination at 150℃ were significantly different in germination at 13℃. The chilling-tolerant lines could be distinguished further at 13℃. The results suggested that the desirable temperature for selecting chilling-tolerant cucumber at germination was 13-15℃. Peroxidase activity was reduced by low temperature, and increased after germination at both 15℃ and 25℃, The two breeding lines. with the best germination at low temperature had an earlier peak in peroxidase activity than other lines. The breeding lines were classified into three groups according to the time of peak peroxidase activity. In addition, complicated changes in peroxidase isozymes occurred during. germination. The number of isozyme bands and their intensities were reduced by low temperature.

Key words: Cucumber, Low temperature germination, POD, Isozyme

中图分类号: 

• S642.201