外源CO对干旱胁迫下黄瓜种子萌发生长的缓解效应

doi:10.7668/hbnxb.2018.05.023

摘要/Abstract

摘要： 为探究外源CO缓解干旱胁迫下黄瓜种子萌发生长的内在机制，以黄瓜品种新津研四号为试材，高铁血红素（0.01 μmol/L）为CO供体，采用PEG-6000（聚乙二醇）模拟干旱胁迫，研究外源CO对干旱胁迫下黄瓜种子萌发生长、养分吸收能力、质膜稳定性、渗透调节物质含量、水解酶活性及其同工酶表达水平的影响。结果表明，外源CO能够提高干旱胁迫下黄瓜种子的发芽势、发芽率和发芽后的根长、胚轴长及鲜质量，能够促进黄瓜种子的吸胀，改进根系活力，显著降低质膜透性和丙二醛（MDA）含量，增加可溶性蛋白和可溶性糖的含量，还能诱导增强淀粉酶和酯酶的活性及相应同工酶的表达水平。与干旱胁迫处理组（PEG）相比，胁迫前施用CO处理组（PEG+CO）的根系活力提高了12.57%，可溶性蛋白和可溶性糖含量分别增加了5.84%和32.56%，淀粉酶和酯酶的活性分别增强了9.87%和48.87%，MDA含量降低了25.81%。这些结果表明，外源CO可以通过增强黄瓜种子的养分吸收能力，保护细胞膜系统的稳定性，改善渗透调节能力，提升水解酶活性及其同工酶的表达水平来提高黄瓜种子对干旱胁迫的适应性，促进种子在干旱胁迫下的萌发和生长。

关键词: 一氧化碳, 干旱胁迫, 黄瓜, 种子萌发, 缓解效应

Abstract: In order to explore the intrinsic mechanism of exogenous carbon monoxide (CO) improving the germination and growth of cucumber seedlings under drought stress. Cucumber Xinjinyan 4 seeds were pretreated with the CO donor,hematin (0.01 mol/L),and their germination,nutrient absorption ability,plasma membrane stability,osmotic adjustment substance content,hydrolase activities and isoenzyme expression under drought stress simulated by 10% polyethylene glycol 6000 were investigated. The results showed that,under PEG stress,exogenous CO improved the germination potential,germination rate,root length,hypocotyl length and fresh weight of cucumber seeds,promoted imbibition of cucumber seeds,enhanced root activity,significantly decreased plasma membrane permeability and malondialdehyde (MDA) content,and increased the contents of soluble protein and soluble sugar. Meanwhile,the results also demonstrated that exogenous CO apparently up-regulated the activities of amylase and esterase and their isozyme expression. Compared with the PEG treatment,the root activity,soluble protein and soluble sugar content,amylase and esterase activity of PEG+CO treatment increased by 12.57%,5.84%,32.56%,9.87% and 48.87%,respectively,MDA content decreased by 25.81%. These results suggested that exogenous CO could improve the adaptability of cucumber seeds to drought stress by enhancing the nutrient absorption ability of cucumber seeds,protecting the stability of cell membrane system,improving osmotic adjustment ability,enhancing the hydrolase activities and their isozyme expression,and consequently induce the germination and growth of cucumber seeds under drought stress.

Key words: Carbon monoxide, Drought stress, Cucumber, Seed germination, Alleviating effects

中图分类号:

牟雪姣, 张强, 吴燕, 王雪娟, 张远兵. 外源CO对干旱胁迫下黄瓜种子萌发生长的缓解效应[J]. 华北农学报, 2018, 33(5): 168-173. doi: 10.7668/hbnxb.2018.05.023.

MU Xuejiao, ZHANG Qiang, WU Yan, WANG Xuejuan, ZHANG Yuanbing. Alleviating Effects of Exogenous CO on Germination and Growth of Cucumber Seeds under Drought Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 168-173. doi: 10.7668/hbnxb.2018.05.023.

http://www.hbnxb.net/CN/Y2018/V33/I5/168

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