摘要： 为了揭示连作藜麦土壤细菌群落结构和多样性的变化，采用高通量测序技术（Illumina-MiSeq）对不同处理（种植1 a、种植2 a）的连作藜麦根际土壤细菌进行16S rRNA基因V4区测序。结果表明，种植1 a较种植2 a的藜麦根际土壤细菌群落丰度和多样性指数都要高，其中，种植1 a较种植2 a的藜麦Chao1指数平均值增加16.4%，ACE指数平均值增加22.9%，Shannon指数平均值增加2.3%；菌群的分类学组成分析结果表明，重茬种植后，伦茨氏菌属、溶杆菌属、中慢生根瘤菌属、丛毛单菌属多样性增多；分枝杆菌属、藤黄单胞菌属、芽单胞菌属、浮霉状菌属等细菌多样性减少。功能预测分析表明，重茬种植后，编码细胞膜转运的功能基因、编码翻译、复制和修复的功能基因、编码外源性物质降解和代谢、多糖生物合成和代谢的功能基因大量减少，编码核苷酸代谢、萜类化合物的代谢、辅因子和维生素的代谢等功能基因少量减少；而编码信号转导和脂类代谢的功能基因有少量增加。

关键词: 藜麦, 连作, 高通量测序, 根际土壤, 生物多样性

Abstract: In order to reveal the changes of bacterial community structure and diversity in quinoa continuous cropping soil, the V4 aera of 16S rRNA gene of bacteria from the quinoa rhizosphere soil with different treatments (planting for 1 a and for 2 a) was sequenced by high throughput sequencing. The results showed that the abundance and diversity index of the bacterial community in rhizosphere soil of planting quinoa for 1 a were higher than those of planting quinoa for 2 a, and the average values of Chao1, ACE and Shannon indexes increased by 16.4%, 22.9% and 2.3%, respectively. The taxonomic composition analysis of bacterial community showed that, after continuous cropping of quinoa, the bacterial diversity of Lentzea, Lysobacter, Mesorhizobium and Polaromonas increased, but the bacterial diversity of Mycobacterium, Luteimonas, Gemmatimonas and Planctomyces decreased. The analysis of functional prediction showed that the functional genes that code the membrane transport, the translation, copy and repair, the degradation and metabolism of exogenous substances and the biosynthesis and metabolism of polysaccharide decreased sigenificantly, and the functional genes coding the metabolism of nucleotide, steroids, cofactors and vitamin also decreased a little, but the functional genes of coding signal transduction and lipid metabolism increased in a small amount.

Key words: Quinoa, Continuous cropping, High-throughput sequencing, Rhizosphere soil, Microbial diversity

中图分类号: 

• S144