摘要： 为筛选与高粱抗丝黑穗病基因相连锁的SRAP分子标记，实现实验室内对高粱抗病性的选择鉴定，达到真正意义的分子标记辅助育种。选用高粱感病品种三尺三和抗病品种Tx622B为研究材料，观察了其形态学和农艺性状，筛选了具有多态性的SRAP标记。结果表明：高粱品种三尺三与Tx622B的田间性状有明显的差异，三尺三的株高、茎粗、秆高、地面茎节数、穗长、穗节数、穗粒重和千粒质量均高于Tx622B，穗一级分支数高于Tx622B，而小区穗数低于Tx622B。2个品种产量性状的差异达到极显著的水平。筛选出16对在抗感品种间有多态性的引物，可用于高粱抗丝黑穗病基因研究。将为高粱抗丝黑穗病优良品种的筛选、培育提供研究基础。

关键词: 高粱, 丝黑穗病, SRAP标记, 抗性基因

Abstract: For screening SRAP markers associated to head smut resistance in sorghum,and to select and identify resistant sorghum in the laboratory,achieving the real molecular marker-assisted breeding,a sensitive sorghum variety Sanchisan,and a resistant variety Tx622B were selected,as research materials. Their morphological and agronomical characters were observed,and the polymorphic SRAP markers were screened. The results showed that there were significant differences in the field characters between Sanchisan and Tx622B,the plant height,stem diameter,culm height,numbers of stem nodes above ground,panical length,numbers of panicle nodes,panicle kernel weight and 1 000 kernel weight of Sanchisan were higher than Tx622B,its primary branch number was higher than Tx622B,in plot was less than Tx622B; The difference of yield traits between the two varieties reached a very significant level. And 16 pairs of polymorphic primers were identified between the two varieties,which can be further used for identifying head smut resistance-associated genes in sorghum. It provided basis for selecting and breeding resistant sorghum varieties to head smut.

Key words: Sorghum, Head smut, SRAP marker, Resistance gene

中图分类号: 

• S332.2

• Q75