水稻种子H<sub>2</sub>O<sub>2</sub>流速和种子活力的关系研究

doi:10.7668/hbnxb.2017.04.030

华北农学报 ›› 2017, Vol. 32 ›› Issue (4): 189-194. doi: 10.7668/hbnxb.2017.04.030

所属专题： 水稻；

水稻种子H₂O₂流速和种子活力的关系研究

李俊周, 李梦琪, 刘磊, 刘娟, 杜彦修, 赵全志

河南粮食作物协同创新中心, 河南省水稻生物学重点实验室, 水稻河南省工程实验室, 河南农业大学, 河南郑州 450002

收稿日期:2017-07-02 出版日期:2017-08-28
通讯作者: 赵全志(1968-),男,河南驻马店人,教授,博士,主要从事水稻栽培生理研究。
作者简介:李俊周(1978-),男,河南开封人,副教授,博士,主要从事水稻遗传育种研究。
基金资助:
河南省重大科技专项（141100110600）；河南省高校科技创新人才支持项目（16HASTIT016）；郑州市节水农业重点实验室建设项目（112PYFZX185）

Study on the Relationship Between H₂O₂ Velocity and Seed Vigor of Rice Seeds

LI Junzhou, LI Mengqi, LIU Lei, LIU Juan, DU Yanxiu, ZHAO Quanzhi

Collaborative Innovation Center of Henan Grain Crops, Henan Key Laboratory of Rice Biology, Henan Engineering Laboratory of Rice, Henan Agricultural University, Zhengzhou 450002, China

Received:2017-07-02 Published:2017-08-28

摘要/Abstract

摘要： 研究了水稻种子H₂O₂流速与种子活力的关系，为快速无损伤鉴别水稻种子活力方法提供依据。以粳稻日本晴、籼稻金农丝苗为材料，采用高温高湿人工老化方法处理种子0，7，10 d获取不同活力水平种子，测量3种类型种子的发芽率、根长、芽长、电导率、H₂O₂含量、CAT活性等性状，并利用非损伤微测系统（NMT）测量种子的H₂O₂流速。结果表明，老化处理造成水稻种子发芽率、根长、芽长和CAT活性降低，而电导率、H₂O₂含量及外排流速增加；老化处理10 d，老化程度加重，趋势更明显。H₂O₂流速与种子发芽率表现极显著负相关的线性关系。种子的H₂O₂流速大小及方向反映了种子内部的氧化还原平衡和细胞膜的状态，通过非损伤微测技术测定H₂O₂流速可以作为水稻种子活力判断的一种方法。

关键词: 水稻, 种子老化, 非损伤微测技术, H₂O₂流速, 种子活力

Abstract: In order to clarify the relationship between H₂O₂ velocity and seed vigor of rice seeds to provide method for rapid and noninvasive identification of rice seed vigor,germination percentage,root length,shoot length,electrical conductivity,H₂O₂ content,CAT activity,and H₂O₂ velocity of rice seeds for japonica Nipponbare and indica Jinnongsimiao after 0,7,10 d of aging times were measured. The results showed that the germination percentage,root length,shoot length and CAT activity were decreased after aging treatment,and the electrical conductivity,H₂O₂ content,and efflux velocity were increased. This trend was more obvious after more serious aging treatment of 10 d. H₂O₂ flow rate and the seed germination percentage showed a significant negative correlation. The magnitude and direction of the H₂O₂ fluxes of the seed reflect the state of redox equilibrium and the cell membrane, H₂O₂ fluxes measured by the non-invasive micro-test technique may be a reliable and sensitive method to evaluate seed germination and vigor.

Key words: Rice, Seed aging, Non-invasive micro-test technique, H₂O₂ flux, Seed vigor

中图分类号:

S511.01

李俊周, 李梦琪, 刘磊, 刘娟, 杜彦修, 赵全志. 水稻种子H₂O₂流速和种子活力的关系研究[J]. 华北农学报, 2017, 32(4): 189-194. doi: 10.7668/hbnxb.2017.04.030.

LI Junzhou, LI Mengqi, LIU Lei, LIU Juan, DU Yanxiu, ZHAO Quanzhi. Study on the Relationship Between H₂O₂ Velocity and Seed Vigor of Rice Seeds[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(4): 189-194. doi: 10.7668/hbnxb.2017.04.030.

http://www.hbnxb.net/CN/Y2017/V32/I4/189

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水稻种子H2O2流速和种子活力的关系研究

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水稻种子H₂O₂流速和种子活力的关系研究

Study on the Relationship Between H₂O₂ Velocity and Seed Vigor of Rice Seeds