摘要： 为得到高效溶磷、促生菌株，对大麦根际土壤的溶磷细菌进行筛选，将筛选得到的高效解磷菌PSM进行分子生物学鉴定，并进行溶磷性能及促生效果研究，为解磷微生物肥料的研发提供依据。结果表明，通过16S rDNA序列分析鉴定表明菌株PSM为枯草芽孢杆菌，35℃在以Ca₃（PO₄）₂为唯一磷源的无机磷液体培养基中培养6 d水溶磷含量达到449.1 mg/L。盆栽试验表明PSM解磷菌对小白菜的促生作用明显，显著提高小白菜的叶绿素、生物产量和叶片数，以添加5.0×10⁸ cfu/g PSM解磷菌剂的处理增产效果最好，鲜质量、干质量与单施复合肥处理（CK1）相比差异极显著，鲜质量增幅达到16.72%，干质量增幅达到18.68%，与单施草炭载体的处理（CK2）相比，施加菌剂的T4处理鲜质量增幅达到11.77%，干质量增幅达到11.70%，有明显的促生作用。

关键词: 溶磷细菌, 筛选鉴定, 枯草芽孢杆菌, 促生

Abstract: In order to obtain efficient dissolving phosphorus and growth-promoting Bacteria of Hordeum vulgare rhizosphere,we took study on screening,P-dissolving characteristics,growth promoting effect and identified by sequence analysis of 16S rDNA of strain PSM,which provided the basis for the development of solubilizing microbial fertilizer. The sequence analysis of 16S rDNA results showed that PSM was Bacillus subtilis,which had strong ability of degradation of inorganic phosphorus effect. After 6 days fermentation at a temperature of 35 degrees in the medium with Ca₃(PO₄)₂ as the sole phosphorus source,soluble phosphorus content reached to 449.1 mg/L. PSM had obvious effect on promoting growth of Brassica campestris L.,the adding of 5.0×10⁸ cfu/g PSM had the best effect on increasing the biological yield and number of leaves significantly. Compared with the single application of compound fertilizer(CK1),fresh weight and dry weight was increased by 16.72% and 18.68% respectively. Compared with the single application of peat carrier(CK2),fresh weight and dry weight was increased by 11.77% and 11.70% respectively. PSM showed evident promoting effect on plant.

Key words: Phosphorus dissolving Bacteria, Screening and identification, Bacillus subtilis, Growth promoting effect

中图分类号: 

• Q939.96