摘要： 为了鉴定一株放线菌G1,并研究其对油菜和白菜的促生长作用.利用16S rDNA通用引物对G1部分基因组进行扩增,对扩增序列进行纯化回收和测序,通过核糖体数据库进行序列比对;利用固态发酵的方法获得G1菌株的孢子粉,并按一定量分别添加到种植有油菜和白菜的盆栽中,50 d后收获,分别测定油菜和白菜的鲜质量、干质量以及叶片SPAD值.确定G1为弗氏链霉菌.油菜盆栽试验中,添加0.2 g G1菌株孢子粉较对照组鲜质量、干质量和SPAD值最高分别增加43.9%,19.2%,6.2%;白菜盆栽试验中,最高分别增加28.8%,29.5%,4.0%.弗氏链霉菌G1对油菜和白菜有明显促生长的作用,具有较高的潜在应用价值.
关键词:
放线菌,
微生物菌剂,
分子鉴定,
促生长
Abstract: In order to identification of a Actinomycetes G1,and to study the growth-promoting effect on oilseed rape and Brassica chinensis.Using 16S rDNA universal primers for G1 part genome amplification.Sequencing and comparison of amplification sequences.Get the G1 spores by solid-state fermentation,and the G1 spores were added to the cultivation of rape and Brassica chinensis in pot.Planting 50 days,rape and Brassica chinensis were determined the fresh weight,dry weight and SPAD value.The G1 strain was identified as Freund's streptomyces.In rape potted plant experiment,add 0.2 g G1 spores,compared with the control group,fresh weight,dry weight,and SPAD highest values were increased 43.9%,19.2%,6.2% respectively.In Brassica chinensis pot experiment,the highest values were increased 28.8%,29.5%,4.0% respectively. Freund's streptomyces G1 obviously promote the growth of rape and Brassica chinensis.It has great potential application value.
Key words:
Actinomyces,
Microorganism agents,
Molecular identification,
Growth-promoting
中图分类号:
高明夫, 邢芳芳, 禚优优, 胡兆平, 李新柱. 一株放线菌G1的鉴定及其对作物促生作用研究[J]. 华北农学报, 2015, 30(S1): 292-295. doi: 10.7668/hbnxb.2015.S1.053.
GAO Ming-fu, XING Fang-fang, ZHUO You-you, HU Zhao-ping, LI Xin-zhu. The Strains of Actinomycetes G1 Appraisal and Its Effect on Crop Growth Research[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(S1): 292-295. doi: 10.7668/hbnxb.2015.S1.053.