摘要： 为获得性状稳定、高产的碱性蛋白酶菌株,采用平板诱变法对产碱性蛋白酶菌株进行诱变处理。结果表明,采用牛奶平板法及Folin测酶活法从鸡粪土样获得产碱性蛋白酶出发菌株。该菌株经亚硝基胍(NTG)诱变并利用利福平抗性株筛选获得蛋白酶活力为出发菌株34,65倍的2个变异株。因此,利用亚硝基胍作为诱变剂可产生高产酶菌株。

关键词: 碱性蛋白酶, 筛选, 诱变育种

Abstract: In order to obtain stable high yield of alkaline protease strains,producing alkaline protease strain was treated by tablet mutagenesis method for mutagenic treatment.The results showed that by using milk tablet and Folin measuring enzyme activities,alkaline protease strains were produced from chicken dung upon sample.The strain by nitrosoguanidine(the NTG mutagenesis)and use of rifampicin resistant strains screened two variants,whose protease activity was 34 times and 65 times of the starting strain.Consequently,mutagen nitrosoguanidine could produce high yield of alkaline protease strains.

Key words: Alkaline protease, Screening, Mutation breeding

中图分类号: 

• Q939.97