摘要： 为了筛选到具有木质部组织特异性并有较强功能的启动子,将河北林木种质资源与森林保护重点实验室前期已获得的MDCesA启动子、JCesAP启动子、DMDCesAP启动子(含2个串联MDCesAP启动子)融合GUS报告基因的pMDCesAP-GUS、pJCesAP-GUS、pDMDCesAP-GUS这3个植物表达载体,通过农杆菌介导转化杨树,对转化后的杨树进行GUS活性的定性及荧光定量检测,以比较3个启动子在转基因植物中的表达能力和水平。筛选到的组织特异启动子与抗天牛基因融合,将提高毒蛋白在相应部位的表达量,这对天牛类蛀干害虫的防治具有重大意义。

关键词: 木质部特异启动子, 筛选, GUS报告基因, 荧光定量检测

Abstract: In order to screen the xylem tissue specific and strong function promoter,three plant expression vector pMDCesAP-GUS,pJCesAP-GUS,pDMDCesAP-GUS,fused with GUS report gene and MDCesA promoter,JCesAP promoter,DMDCesAP promoter (including two series MDCesAP promoter) acquired earlier,are transformed to poplar by agrobacterium mediated transformation.With qualitative and quantitative fluorescence detection of GUS activity,the three promoter in transgenic plants expressing ability and level are detected.Fusion of the screened xylem specific promoter and insect-resistant gene will improve the poisonous protein expression in the xylem of the quantity,which is of great significance for prevention and control of boring beetles.

Key words: Xylem specific promoter, Screening, GUS reporter gene, Fluorescence quantitative detection

中图分类号: 

• Q78