摘要： 为了更快更准确地在病菌潜伏期或发病初期鉴定出尖孢镰刀菌引起的瓜类枯萎病和茄病镰刀菌引起的瓜类根腐病这2种病害,根据尖孢镰刀菌和茄病镰刀菌的翻译延长因子TEF1-α序列设计了3条种特异性引物。引物对FO-F和Fu-R可以从尖孢镰刀菌中扩增到1条228 bp的片段,引物对FS-F和Fu-R可以从茄病镰刀菌中扩增到1条347 bp片段,并且3条引物FO-F、FS-F和Fu-R可以同时在1个PCR反应中扩增到2个片段,而这2对引物都不能从其他病原真菌中扩增到任何条带。结果表明,该多重PCR检测方法可以同时鉴定样品中的2种镰刀菌。
关键词:
尖孢镰刀菌,
茄病镰刀菌,
多重PCR检测,
葫芦科作物,
TEF-1α
Abstract: The early symptoms caused by F.oxysporum and F.solani were similar in cucurbitaceous plants such as watermelon.To rapidly and accurately distinguish the two diseases at a potential or early infection,three primes were designed based on the TEF1-α(Translation elongation factor 1-α) of F.oxysporum and F.solani. The primer pair FO-F+Fu-R amplified a 228 bp fragment from F.oxysporum,and the primer pair FS-F+Fu-R amplified a 347 bp fragment from F.solani.No PCR products were amplified with these primer pairs from DNA of other fungal species.A multiple species-specific PCR method was developed successfully to detect both two fusarium fungi simultaneously in single PCR amplifications with the primers FO-F+FS-F+Fu-R.
Key words:
F.oxysporum,
F.solani,
Multiple PCR detection,
Cucurbitaceous plants,
TEF-1α
中图分类号:
严蕾艳, 张宴瑜, 马凯慧, 宋慧, 王毓洪. 两种瓜类镰刀菌的多重PCR检测[J]. 华北农学报, 2014, 29(S1): 44-47. doi: 10.7668/hbnxb.2014.S1.010.
YAN Lei-yan, ZHANG Yan-yu, MA Kai-hui, SONG Hui, WANG Yu-hong. Multiple PCR Detection of Fusarium oxysporum and Fusarium solani in Cucurbitaceous Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 44-47. doi: 10.7668/hbnxb.2014.S1.010.