摘要： 为了克隆 DL-6 基因,以dl-6与9311配制正反杂交组合进行性状分析发现,dl-6 突变性状受1对隐性核基因控制,利用SSR分子标记将控制dl-6性状的基因 DL-6 初步定位在水稻第3染色体的短臂上,进一步利用新发展的InDel标记将 DL-6 基因精细定位在I3-5和I3-8之间85 kb的物理距离内。对该区段内存在的开放阅读框(ORF)进行分析,发现其中ORF9编码的 YABBY 基因是一个与叶脉发育相关的基因,对突变体dl-6和野生型中 YABBY 基因进行测序,将测序结果与数据中日本晴序列进行比对发现,突变体dl-6中 YABBY 基因的第1个外显子存在1个单碱基突变,该突变导致野生型中编码的半胱氨酸突变为突变体中的精氨酸;同时突变体dl-6在 YABBY 基因的3'端还存在8个碱基的缺失。这2个突变位点哪个是导致dl-6突变的功能区目前还不确定,有待进一步研究。

关键词: 水稻, 中脉缺陷, 基因定位, dl-6

Abstract: To clone the DL-6 locus,positive and negative cross combinations were developed between dl-6 and 9311,and genetic analysis found that drooping leaf was controlled by one recessive nuclear gene DL-6. DL-6 was primarily mapped on the short arm of chromosome 3 with SSR markers.With newly developed markers DL-6 was finally fine mapped in 85 kb region between markers I3-5 and I3-8.Open reading frame (ORF) analysis revealed that ORF9 might be a drooping leaf related gene.ORF9 both in mutant dl-6 and in wild type were sequenced and 1 bp mutation was found in first exon in dl-6 when blasted with Nipponbare.Cysteine mutated into arginine in dl-6.Meanwhile,8 bp deletion was also found at 3' end of YABBY.These two mutations which one is the functional mutation of DL-6 is still uncertain and need deeply research.

Key words: Rice, Midrib-deficient, Gene mapping, dl-6

中图分类号: 

• Q78

• S511.03