利用双尾法定位大豆水溶性蛋白QTL

doi:10.7668/hbnxb.2014.04.013

华北农学报 ›› 2014, Vol. 29 ›› Issue (4): 80-86. doi: 10.7668/hbnxb.2014.04.013

所属专题： 油料作物；

利用双尾法定位大豆水溶性蛋白QTL

陈强¹, 雷雅坤³, 闫龙², 邓莹莹¹, 杨春燕², 乔亚科¹, 刘兵强², 刘青², 唐晓东², 张孟臣^1,2

1. 河北科技师范学院生命科技学院, 河北昌黎 066600;
2. 河北省农林科学院粮油作物研究所, 国家大豆改良中心石家庄分中心, 农业部黄淮海大豆生物学与遗传育种重点实验室, 河北省遗传育种重点实验室, 河北石家庄 050035;
3. 河北省农林科学院农业信息与经济研究所, 河北石家庄 050051

收稿日期:2014-05-07 出版日期:2014-08-28
通讯作者: 张孟臣（1956-），男，河北衡水人，研究员，硕士，主要从事大豆遗传育种研究。
作者简介:陈强（1987-），男，河北博野人，在读硕士，主要从事大豆分子数量遗传与育种研究。
基金资助:
国家“十二五”科技支撑计划项目（2011BAD35B06）；国家自然科学基金项目（31201234）；河北省博士基金项目（F13E006）

QTL Mapping of Soluble Protein Content in Soybean by Two Tail Analysis

CHEN Qiang¹, LEI Ya-kun³, YAN Long², DENG Ying-ying¹, YANG Chun-yan², QIAO Ya-ke¹, LIU Bing-qiang², LIU Qing², TANG Xiao-dong², ZHANG Meng-chen^1,2

1. College of Biological Science and Technology, Hebei Normal University of Science Technology, Changli 066600, China;
2. Hebei Academy of Agriculture and Forestry Sciences, National Soybean Improvement Center Shijiazhuang Sub-Center, North China Key Laboratory of Soybean Biology and Genetic Improvement, Ministry of Agriculture, Cereal & Oil Crop Institute, Shijiazhuang 050035, China;
3. Institute of Agricultural Information and Economy, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

Received:2014-05-07 Published:2014-08-28

摘要/Abstract

摘要： 大豆水溶性蛋白是大豆籽粒蛋白质的主要组成部分，其含量是评价大豆品质及衡量其价值的重要指标。以高水溶性蛋白含量（38.70±1.32）%的冀豆12和正常水溶性蛋白含量（32.40±1.08）%的黑豆（ZDD03651）为亲本构建的包含188个家系的F₈、F₉重组自交系群体为材料，利用双尾法在3个不同选择压力下对籽粒水溶性蛋白含量进行QTL定位研究。结果表明，2年中重组自交家系群体表型均呈现正态性超双亲分离，在30%，20%，10%这3个不同选择压力下共检测到大豆籽粒水溶性蛋白含量相关的QTL 23个，分布在A1、A2、C2、D1b、D2、E、F、H、I、K、L、M和O等13个连锁群中，其中13个QTL增效基因来自冀豆12。2年中在10%的选择压力下同时检测到2个QTL，分别为 qSPC-6-1和qSPC-8-1。其中 qSPC-6-1 的连锁标记为Sat_062，2年中贡献率分别为9.78%和9.66%，增效基因来自冀豆12；qSPC-8-1 的连锁标记为Satt177，2年中贡献率分别为9.78%和7.98%，增效基因来自于黑豆。该结果为分子标记辅助育种及分子克隆提供理论依据。

关键词: 大豆, 水溶性蛋白, 双尾法, QTL

Abstract: Water soluble protein is the main component of soybean seed protein,the percentage of water soluble protein is an important index to value measure and quality evaluation.Two tail analysis in three selection pressure(30%,20%,10%)were employed to detect quantitative trait loci(QTL)of soybean soluble protein content using 188 recombination inbred lines(RIL)population derived from a cross between Jidou 12 and Heidou,Jidou 12 has high SPC(38.70±1.32)% and Heidou has low SPC(32.40±1.08)%.As a result,SPC phenotypic was appeared to be normal transgressive segregation distribution.And 23 QTLs were detected in 3 selection pressure(30%,20%,10%),distribution on A1,A2,C2,D1b,D2,E,F,H,I,K,L,M and O linkage group,13 of those favorable genes from Jidou 12.There were 2 QTL qSPC-6-1, qSPC-8-1 were detected by the two years in 10% selection pressure.The linkage marker of qSPC-6-1 was Sat_062,in two years genetic contribution is 9.78% and 9.66% respectively and favorable gene from Jidou12;The linkage marker of qSPC-8-1 was Satt177,in two years genetic contribution is 9.78% and 7.98% respectively and favorable gene from Heidou.These studies provide a theoretical basis for marker-assisted breeding.

Key words: Soybean, Soluble protein, Two tail analysis, QTL

中图分类号:

S565.03

陈强, 雷雅坤, 闫龙, 邓莹莹, 杨春燕, 乔亚科, 刘兵强, 刘青, 唐晓东, 张孟臣. 利用双尾法定位大豆水溶性蛋白QTL[J]. 华北农学报, 2014, 29(4): 80-86. doi: 10.7668/hbnxb.2014.04.013.

CHEN Qiang, LEI Ya-kun, YAN Long, DENG Ying-ying, YANG Chun-yan, QIAO Ya-ke, LIU Bing-qiang, LIU Qing, TANG Xiao-dong, ZHANG Meng-chen. QTL Mapping of Soluble Protein Content in Soybean by Two Tail Analysis[J]. journal1, 2014, 29(4): 80-86. doi: 10.7668/hbnxb.2014.04.013.

http://www.hbnxb.net/CN/Y2014/V29/I4/80

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