摘要： 为了明确辣椒花青素生物合成途径中相关基因的表达调控模式，选取3种不同果实颜色的辣椒品系（紫晶、淡紫、水晶）为试材，提取果实不同发育阶段的RNA并反转录成cDNA，利用qRT-PCR技术分析相关基因（CHS、CHI、F3H、F3'5'H、DFR、ANS、UFGT、ANP、GST）在不同辣椒材料果实不同发育阶段的表达情况。结果显示：CHS、CHI 在3种材料果实发育过程中表达量都很少且无规律可循，F3H 在淡紫、紫晶中表达量较高，且在果实发育的第15~20天时出现较大峰值，与之相比在水晶中的表达量可以忽略不计。 F3'5'H、ANP、GST、DFR、UFGT 在3种材料中相对表达量随着果实的发育分别呈现同步变化，且 F3'5'H、ANP、GST在材料紫晶果实发育的第15天相对表达量出现较大峰值，ANS在3种材料果实发育过程中的相对表达量变化没有一定规律可循。 F3H、F3'5'H、ANP、GST是辣椒花青素代谢途径的关键基因。

关键词: 辣椒, 花青素, 生物合成, 表达分析

Abstract: In order to clarify the expression and regulation patterns of related gene in the process of anthocyanin biosynthesis in pepper,select three pepper strains which fruit of different color for materials(Zijing,Danzi,Shuijing),extract RNA of different stages of the fruit and reverse transcribed into cDNA,then using qRT-PCR technology to analysis of expression of structure genes (CHS, CHI, F3H, F3'5'H, DFR,ANS, UFGT, ANP and GST) in pepper fruit of three kinds of materials in different development stages.The results showed that the expression of CHS,CHI are rarely and disorder in the process of fruit development of three kinds of materials,the expression of F3H is higher in Danzi and Zijing,and appear larger peak in the fruit development of 15-20 days,compared with it,the amount of expression in Shuijing can be negligible.The relative transcription level of F3'5'H, ANP,GST, DFR and UFGT express synchronous change in three kinds of materials,and the relative expression of F3'5'H, ANP and GST in the 15th day of Zijing fruit appeared larger peak,the relative transcription level of ANS without some rules to follow. F3H, F3'5'H, ANP and GST are the key genes to the anthocyanins metabolic pathway in pepper.

Key words: Pepper, Anthocyanins, Biosythesis, Expression analysis

中图分类号: 

• Q78