水稻早抽穗突变体ehd(t)的遗传分析和分子定位

doi:10.7668/hbnxb.2011.06.011

摘要/Abstract

摘要： 水稻品种的抽穗期是重要农艺性状之一,鉴定和定位抽穗期基因,分析其遗传效应对改良水稻抽穗期至关重要。在水稻品种Nipponbare中发现一个早抽穗植株,经过多代自交获得稳定的早抽穗突变株,突变体ehd(t)比Nipponbare早抽穗15 d。遗传分析表明,该突变受1对显性核基因控制,暂命名为EHD(t)。通过2个F₂定位群体,利用微卫星标记将EHD(t)定位于第8号染色体Rm²23和RM584之间,其遗传距离分别为5.2 cM和6.1 cM。

关键词: 水稻, 早抽穗基因, 遗传分析, 基因定位

Abstract: Rice heading date is an important agronomic trait． Mapping heading date gene is important for rice improvement． A rice(Oryza sativaL．) mutant with a early heading date，designated as edh(t),was found from a japonicarice variety Nipponbare． This early heading date mutant phenotype was stably expressed through successive five self-crossed generations，and its heading date was earlier 15 days than that in wild-type． Genetic analysis showed that the phenotype of edh(t) was controlled by a single dominant nuclear gene， temporarily designated as ehd(t). By means of molecular marker technique， the ehd(t)gene was mapped to an interval between two SSR markers RM223 and RM584 on chromosome 8 with two F₂segregating population，with genetic distances of 5． 2 cM and 6． 1 cM to each marker respectively． This is the basis for marker-assisted selection and map-based coloning this ehd(t)gene．

Key words: Rice, Early heading date gene, Genetic analysis, Molecular mapping

中图分类号:

李万昌, 姜丽娜, 王俊伟, 余娇娇. 水稻早抽穗突变体ehd(t)的遗传分析和分子定位[J]. 华北农学报, 2011, 26(6): 55-57. doi: 10.7668/hbnxb.2011.06.011.

LI Wan-chang, JIANG Li-na, WANG Jun-wei, YU Jiao-jiao. Genetic Analysis and Molecular Mapping of A Early Heading Date Mutant(ehd(t)) in Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 55-57. doi: 10.7668/hbnxb.2011.06.011.

http://www.hbnxb.net/CN/Y2011/V26/I6/55

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