摘要： 在网室盆栽条件下,采用3水平灌水量(40,60,80 L/株)×4水平施氮量(0,3,7,12 g/株),以烤烟K326为试验材料,研究了水氮耦合对烤烟生育期内土壤过氧化氢酶、蔗糖酶、脲酶和酸性磷酸酶活性动态变化的影响。结果表明,植烟土壤4种酶活性随生育时期推进均呈先上升后下降的趋势,且在旺长期或现蕾期达最高。在相同生育时期和相同灌水水平下,随着施氮水平的提高,植烟土壤过氧化氢酶、蔗糖酶和酸性磷酸酶活性均呈先增加后降低的变化趋势,且过氧化氢酶和蔗糖酶以3 g/株施氮水平下活性最高,酸性磷酸酶活性以7 g/株施氮水平达最高;而脲酶活性则随着施氮量的增加而增加,在12 g/株施氮水平下达最高。在相同生育时期和相同施氮量下,以灌水处理为60L/株和80 L/株的植烟土壤4种酶活性均较高,而低灌水处理(40 L/株)的土壤酶活性均较低。综合所有指标,在该试验条件下,当灌水量为60～80 L/株、施氮量3～7 g/株,烤烟生育期内4种土壤酶均保持较高的活性。

关键词: 水氮耦合, 烤烟, 酶活性

Abstract: In this article,flue-cured tobacco(Nicotiana tobacum K326)was selected to study effects of water and nitrogen coupling on dynamic changes of soil enzymatic activities of catalase,sucrase,urease and acid phosphatase during the growth stage of flue-cured tobacco by net house cultivation with conditions of 3 levels of water irrigation(40,60 and 80 L/plant)and 4 levels of nitrogen application(0,3,7 and 12 g/plant).The results showed,the soil enzymatic activities of catalase,sucrase,urease and acid phosphatase increased first and then decreased with the advance of growth stage in flue-cured tobacco,and maximized at vigorous growing stage or budding stage.With the same growth period and the same water irrigation,the soil enzyme activities of catalase,sucrase and acid phosphatase increased first and then decreased with the advance of growth stage in flue-cured tobacco,while the urease activity of soil increased continuously with the advance of growth stage.Activities of the catalase and sucrase peaked at 3 g/plant,and acid phosphatase activity peaked at 7 g/plant and the urease activity peaked at 12 g/plant.With the same growth period and the same nitrogen application,above four soil enzyme activities were all higher with the range of 60-80 L/plant of water irrigation,while they were lowest with the level of 40 L/plant of water irrigation.In a word,the four of soil enzymatic activities were higher with the range of 60-80 L/plant and nitrogen 3-7 g/plant.

Key words: Water and nitrogen coupling, Flue-cured tobacco, Soil enzymatic activities

中图分类号: 

• S572